Team:Caltech/Project/Methods and Methods

From 2014.igem.org

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<ul><li>For each 25 uL reaction</li>
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For each 25 uL reaction mixture:
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     <li></li>
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<ul><li>12.5 uL Phusion Mastermix</li>
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     <li>2.5 uL primer mix (10 uM of forward and reverse primer)</li>
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    <li>1 uL DNA template </li>
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    <li>0.75 uL DMSO (optional) </li>
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    <li>Fill to 25 uL with MilliQ water </li>  
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</ul>
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Revision as of 20:35, 14 July 2014



Home Team Official Team Profile Project Parts Modeling Notebook Safety Attributions
Materials and Methods
Overall Project Summary

Project Details

Materials and Methods

The Experiments

Results

Data Analysis

Conclusions

References

PCR

For each 25 uL reaction mixture:
  • 12.5 uL Phusion Mastermix
  • 2.5 uL primer mix (10 uM of forward and reverse primer)
  • 1 uL DNA template
  • 0.75 uL DMSO (optional)
  • Fill to 25 uL with MilliQ water

Gel electrophoresis

  • blah blah

Colony PCR

  • blah blah
  • blah blah
  • blah blah
  • blah blah

Gibson assembly

  • whreee
  • blah blah
  • blah blah
  • blah blah
  • blah blah

PCR purification

  • Used protocol and kits provided by Qiagen