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Team:TU Darmstadt/Materials/Media
From 2014.igem.org
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<!--TYPO3SEARCH_begin--><div id="c74" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">Media</h1></div><div><header><p><b>1.7-DYT (recipe for 500 mL)</b></p></header></div><div></div><div><p>- 2.5 g NaCl | <!--TYPO3SEARCH_begin--><div id="c74" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">Media</h1></div><div><header><p><b>1.7-DYT (recipe for 500 mL)</b></p></header></div><div></div><div><p>- 2.5 g NaCl | ||
</p> | </p> | ||
| - | <p>- 8.0 g tryptone</p></div><div><p>- 5.0 g yeast extract</p></div><div><p>- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)</p></div><div></div><div><p><b>LB (recipe for 1000 mL)</b></p></div><div></div><div><p>- 10 g tryptone</p></div><div><p>- 5 g yeast extract</p></div><div><p>- 10 g NaCl</p></div><div><p>- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)</p></div><div><p>For preparing plates add 15 g agar.</p></div><div></div><div><p><b>SOB (recipe for 1000 mL)</b></p></div><div></div><div><p>- 0.186 g KCl</p></div><div><p>- 2.4 g MgSO4</p></div><div><p>- 0.584 g NaCl</p></div><div><p>- 20 g tryptone</p></div><div><p>- 5 g yeast extract</p></div><div><p>- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)</p></div><div></div><div><p> | + | <p>- 8.0 g tryptone</p></div><div><p>- 5.0 g yeast extract</p></div><div><p>- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)</p></div><div></div><div><p><b>LB (recipe for 1000 mL)</b></p></div><div></div><div><p>- 10 g tryptone</p></div><div><p>- 5 g yeast extract</p></div><div><p>- 10 g NaCl</p></div><div><p>- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)</p></div><div><p>For preparing plates add 15 g agar.</p></div><div></div><div><p><b>SOB (recipe for 1000 mL)</b></p></div><div></div><div><p>- 0.186 g KCl</p></div><div><p>- 2.4 g MgSO4</p></div><div><p>- 0.584 g NaCl</p></div><div><p>- 20 g tryptone</p></div><div><p>- 5 g yeast extract</p></div><div><p>- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)</p></div><div></div><div><p></p> |
| - | <p><b>SOC (recipe for 1000 mL)</b></p></div><div></div><div><p>- 980 mL SOB</p></div><div><p>- 20 mL 1M Glucose</p></div><div></div><div></div><div> | + | <p><b>SOC (recipe for 1000 mL)</b></p></div><div></div><div><p>- 980 mL SOB</p></div><div><p>- 20 mL 1M Glucose</p></div><div></div><div></div><div></p> |
<p><b>Autoclave after mixing.</b></p></div></div><!--TYPO3SEARCH_end--> | <p><b>Autoclave after mixing.</b></p></div></div><!--TYPO3SEARCH_end--> | ||
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Revision as of 16:52, 17 October 2014
Media
1.7-DYT (recipe for 500 mL)
- 2.5 g NaCl
- 8.0 g tryptone
- 5.0 g yeast extract
- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)
LB (recipe for 1000 mL)
- 10 g tryptone
- 5 g yeast extract
- 10 g NaCl
- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)
For preparing plates add 15 g agar.
SOB (recipe for 1000 mL)
- 0.186 g KCl
- 2.4 g MgSO4
- 0.584 g NaCl
- 20 g tryptone
- 5 g yeast extract
- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)
SOC (recipe for 1000 mL)
- 980 mL SOB
- 20 mL 1M Glucose
Autoclave after mixing.
