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Team:Bielefeld-CeBiTec/Results/CO2-fixation
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| - | The particular aim of the second module is to | + | The particular aim of the second module is to implement the carbon dioxide fiaxtion in <i>E. coli</i>. For this approach all items, like the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/Calvin-Cycle" target="_blank">sedoheptulose-1,7-bisphosphatase</a>, the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/RuBisCO" target="_blank">ribulose-1,5-bisphosphate carboxylase/oxygenase</a> (RuBisCO) and the mechanism of <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/RuBisCO" target="_blank">carbon dioxide fixation</a> were tested separetly in various approaches. For the optimization of the carbon dioxide fixation under aerobic growth conditions we investigate the anerobic microcompartment from <i>Halothiobacillus neapolitanus</i>, called the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/Carboxysome" target="_blank">carboxysom</a>. |
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<a href="https://static.igem.org/mediawiki/2014/5/54/Bielefeld-CeBiTec_2014-10-11_Carboxy_weiss_wiki.png" target="_blank"><img src="https://static.igem.org/mediawiki/2014/5/54/Bielefeld-CeBiTec_2014-10-11_Carboxy_weiss_wiki.png" width="450px"></a><br> | <a href="https://static.igem.org/mediawiki/2014/5/54/Bielefeld-CeBiTec_2014-10-11_Carboxy_weiss_wiki.png" target="_blank"><img src="https://static.igem.org/mediawiki/2014/5/54/Bielefeld-CeBiTec_2014-10-11_Carboxy_weiss_wiki.png" width="450px"></a><br> | ||
| - | <font size="2" style="text-align:center;"><b> | + | <font size="2" style="text-align:center;"><b>Figure 1:</b> Schematic representation of the Calvin cylce. The reaction shown in green can be catalyzed by enzymes that naturally exist in <i>E. coli</i>, while the red one need to be expressed heterologous to enable the whole <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/Calvin-Cycle">Calvin cycle</a> in <i>E. coli</i>.</font> |
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| - | For this purpose we made different approaches. First of all we identified two <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/RuBisCO">RuBisCOs</a> (from <i> | + | For this purpose we made different approaches. First of all we identified two <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/RuBisCO">RuBisCOs</a> (from <i>H. neapolitanus</i> and from <i>Synechococcus elongatus</i>) as the most efficient and best fitting ones. There coding sequences were synthesized to remove illegal restriction sites and to optimize the codong usage for the heterologous expression in <i>E. coli</i>. These enzymes are known to function best inside a carboxysome. Therefore we started the construction of such a microcompartment in <i>E. coli</i>. |
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Revision as of 22:00, 17 October 2014
Module II - Carbon Dioxide (CO2) Fixation
The particular aim of the second module is to implement the carbon dioxide fiaxtion in E. coli. For this approach all items, like the sedoheptulose-1,7-bisphosphatase, the ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) and the mechanism of carbon dioxide fixation were tested separetly in various approaches. For the optimization of the carbon dioxide fixation under aerobic growth conditions we investigate the anerobic microcompartment from Halothiobacillus neapolitanus, called the carboxysom.
Figure 1: Schematic representation of the Calvin cylce. The reaction shown in green can be catalyzed by enzymes that naturally exist in E. coli, while the red one need to be expressed heterologous to enable the whole Calvin cycle in E. coli.
