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Team:Groningen/Template/MODULE/Notebook/detection/week6
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| - | + | September 1 - September 7 | |
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| - | + | The size of the construct was determined by colony PCR | |
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| - | + | The cultures, which showed positive results for the colony PCR were inoculated in fresh LB media containing the corresponding antibiotics | |
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| - | + | Two new ligations were performed, the <i>lasR</i> double terminator was ligated with Promoter CP29, and the gene coding for GFP double terminator was ligated with the RBS | |
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| - | + | The two new construct were both transformed to two different batches of <i>E. coli</i> DH5α | |
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Revision as of 19:59, 17 October 2014
September 1 - September 7
The size of the construct was determined by colony PCR
The cultures, which showed positive results for the colony PCR were inoculated in fresh LB media containing the corresponding antibiotics
Two new ligations were performed, the lasR double terminator was ligated with Promoter CP29, and the gene coding for GFP double terminator was ligated with the RBS
The two new construct were both transformed to two different batches of E. coli DH5α
Colony PCR to check the size.
