Team:Groningen/Template/MODULE/Notebook/detection/week6

The size of the construct was determined by colony PCR

The cultures, which showed positive results for the colony PCR were inoculated in fresh LB media containing the corresponding antibiotics

Two new ligations were performed, the lasR double terminator was ligated with Promoter CP29, and the gene coding for GFP double terminator was ligated with the RBS

The two new construct were both transformed to two different batches of E. coli DH5α

The size of the construct was checked by colony PCR