Team:LIKA-CESAR-Brasil/ImmoblizationProcess
From 2014.igem.org
Villaverde (Talk | contribs) (Created page with "{{CSS/Main}} <html lang="pt-br"> <head> <meta charset="utf-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge"> <meta name="viewport" content="width=device-wid...") |
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<link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.2.0/css/bootstrap.min.css"> | <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.2.0/css/bootstrap.min.css"> | ||
- | < | + | <style type="text/css"> |
+ | body { | ||
+ | font-size:12px; | ||
+ | color:#111; | ||
+ | height:100%; | ||
+ | background:#ededed; | ||
+ | /*min-width: 1326px;*/ | ||
+ | } | ||
+ | .container { | ||
+ | width:80%; | ||
+ | background: rgb(255,255,255); /* Old browsers */ | ||
+ | background: -moz-linear-gradient(top, rgba(255,255,255,1) 0%, rgba(237,237,237,1) 100%); /* FF3.6+ */ | ||
+ | background: -webkit-gradient(linear, left top, left bottom, color-stop(0%,rgba(255,255,255,1)), color-stop(100%,rgba(237,237,237,1))); /* Chrome,Safari4+ */ | ||
+ | background: -webkit-linear-gradient(top, rgba(255,255,255,1) 0%,rgba(237,237,237,1) 100%); /* Chrome10+,Safari5.1+ */ | ||
+ | background: -o-linear-gradient(top, rgba(255,255,255,1) 0%,rgba(237,237,237,1) 100%); /* Opera 11.10+ */ | ||
+ | background: -ms-linear-gradient(top, rgba(255,255,255,1) 0%,rgba(237,237,237,1) 100%); /* IE10+ */ | ||
+ | background: linear-gradient(to bottom, rgba(255,255,255,1) 0%,rgba(237,237,237,1) 100%); /* W3C */ | ||
+ | filter: progid:DXImageTransform.Microsoft.gradient( startColorstr='#ffffff', endColorstr='#ededed',GradientType=0 ); /* IE6-9 */ | ||
+ | } | ||
+ | |||
+ | .navbar-brand { | ||
+ | padding: 5px; | ||
+ | } | ||
+ | .col-md-10 { | ||
+ | width:100%; | ||
+ | padding: 20px 70px 20px 50px; | ||
+ | min-height:200px; | ||
+ | padding-bottom:50px; | ||
+ | } | ||
+ | .col-md-4 { | ||
+ | padding-bottom:70px; | ||
+ | } | ||
+ | .navbar-inverse { | ||
+ | background-color: #ededed; | ||
+ | border-color: #ededed; | ||
+ | } | ||
+ | .navbar-inverse .navbar-nav > .active > a, .navbar-inverse .navbar-nav > .active > a:hover, .navbar-inverse .navbar-nav > .active > a:focus { | ||
+ | color: #006600; | ||
+ | background-color: #8cff00; | ||
+ | } | ||
+ | .navbar-inverse .navbar-nav > .open > a, .navbar-inverse .navbar-nav > .open > a:hover, .navbar-inverse .navbar-nav > .open > a:focus { | ||
+ | color: #006600; | ||
+ | background-color: #8cff00; | ||
+ | } | ||
+ | .navbar-inverse .navbar-nav > li > a:hover, .navbar-inverse .navbar-nav > li > a:focus { | ||
+ | color: #006600; | ||
+ | background-color: transparent; | ||
+ | } | ||
+ | .navbar-inverse .navbar-nav > li > a:focus { | ||
+ | color: #006600; | ||
+ | background-color: transparent; | ||
+ | } | ||
+ | .nav > li > a { | ||
+ | display: block; | ||
+ | padding: 25px 15px; | ||
+ | position: relative; | ||
+ | } | ||
+ | .menu{ | ||
+ | color:black; | ||
+ | z-index:999; | ||
+ | position:relative; | ||
+ | float:right; | ||
+ | margin:auto; | ||
+ | } | ||
+ | |||
+ | .videohome{ | ||
+ | background:#ededed; | ||
+ | } | ||
+ | .menu ul{ | ||
+ | margin:0 auto; | ||
+ | } | ||
+ | .jumbotron { | ||
+ | background:url("https://static.igem.org/mediawiki/2014/7/7a/Banner_top.jpg"); | ||
+ | border-top: solid 5px #8cff00; | ||
+ | border-bottom: solid 5px #8cff00; | ||
+ | background-size:cover; | ||
+ | width:80%; | ||
+ | height:231px; | ||
+ | margin:auto; | ||
+ | margin-top:40px; | ||
+ | } | ||
+ | .jumbotron02 { | ||
+ | background:url("https://static.igem.org/mediawiki/2014/5/52/Banner_top02.jpg"); | ||
+ | border-top: solid 5px #8cff00; | ||
+ | border-bottom: solid 5px #8cff00; | ||
+ | background-size:cover; | ||
+ | width:80%; | ||
+ | height:231px; | ||
+ | margin:auto; | ||
+ | margin-top:80px; | ||
+ | } | ||
+ | .jumbotronSafety { | ||
+ | background:url("https://static.igem.org/mediawiki/2014/7/7a/Banner_top.jpg"); | ||
+ | border-top: solid 5px #8cff00; | ||
+ | border-bottom: solid 5px #8cff00; | ||
+ | background-size:cover; | ||
+ | width:80%; | ||
+ | height:231px; | ||
+ | margin:auto; | ||
+ | margin-top:80px; | ||
+ | } | ||
+ | .jumbotronParts { | ||
+ | background:url("https://static.igem.org/mediawiki/2014/b/be/Banner_top04.jpg"); | ||
+ | border-top: solid 5px #8cff00; | ||
+ | border-bottom: solid 5px #8cff00; | ||
+ | background-size:cover; | ||
+ | width:80%; | ||
+ | height:231px; | ||
+ | margin:auto; | ||
+ | margin-top:80px; | ||
+ | } | ||
+ | .jumbotronHuman { | ||
+ | background:url("https://static.igem.org/mediawiki/2014/1/12/Banner_top05.jpg"); | ||
+ | border-top: solid 5px #8cff00; | ||
+ | border-bottom: solid 5px #8cff00; | ||
+ | background-size:cover; | ||
+ | width:80%; | ||
+ | height:231px; | ||
+ | margin:auto; | ||
+ | margin-top:80px; | ||
+ | } | ||
+ | .jumbotronProject { | ||
+ | background:url("https://static.igem.org/mediawiki/2014/b/be/Banner_top04.jpg"); | ||
+ | border-top: solid 5px #8cff00; | ||
+ | border-bottom: solid 5px #8cff00; | ||
+ | background-size:cover; | ||
+ | width:80%; | ||
+ | height:231px; | ||
+ | margin:auto; | ||
+ | margin-top:80px; | ||
+ | } | ||
+ | .jumbotronAdvisors { | ||
+ | background:url("https://static.igem.org/mediawiki/2014/3/3b/Banner_top03.jpg"); | ||
+ | border-top: solid 5px #8cff00; | ||
+ | border-bottom: solid 5px #8cff00; | ||
+ | background-size:cover; | ||
+ | width:80%; | ||
+ | height:231px; | ||
+ | margin:auto; | ||
+ | margin-top:80px; | ||
+ | } | ||
+ | |||
+ | .igem-logo{ | ||
+ | background:url("https://static.igem.org/mediawiki/2014/4/4e/Banner_top_03.png") ; | ||
+ | background-color:transparent; | ||
+ | width: 50px; | ||
+ | height:50px; | ||
+ | z-index: 99; | ||
+ | background-repeat: no-repeat; | ||
+ | } | ||
+ | |||
+ | /* | ||
+ | .navbar .container { | ||
+ | min-width: 1322px; | ||
+ | z-index: 9999; | ||
+ | } | ||
+ | */ | ||
+ | |||
+ | h2 { | ||
+ | font-size: 26px; | ||
+ | color:#339900; | ||
+ | } | ||
+ | .videocapa{ | ||
+ | width:35%; | ||
+ | float:right; | ||
+ | min-height:300px; | ||
+ | margin-top:10px; | ||
+ | } | ||
+ | p { | ||
+ | font-size: 12px; | ||
+ | color:#444; | ||
+ | } | ||
+ | footer { | ||
+ | background:#b8b8b8; | ||
+ | width:100%; | ||
+ | padding: 50px; | ||
+ | clear:both; | ||
+ | position: relative; | ||
+ | bottom:0; | ||
+ | border-top: solid 8px #8cff00; | ||
+ | } | ||
+ | footer p { | ||
+ | margin:auto; | ||
+ | padding-top:10px; | ||
+ | text-align:center; | ||
+ | width:50%; | ||
+ | color:#006600; | ||
+ | } | ||
+ | .firstHeading { | ||
+ | display:none; | ||
+ | } | ||
+ | table{ | ||
+ | color:#888; | ||
+ | font-size:16px; | ||
+ | border: solid 1px #ccc; | ||
+ | } | ||
+ | h1 { | ||
+ | font-size: 26px; | ||
+ | color:#339900; | ||
+ | } | ||
+ | h2 { | ||
+ | font-size: 22px; | ||
+ | color:#339900; | ||
+ | } | ||
+ | h3 { | ||
+ | font-size: 18px; | ||
+ | color:#339900; | ||
+ | } | ||
+ | h4 { | ||
+ | font-size: 16px; | ||
+ | color:#339900; | ||
+ | } | ||
+ | |||
+ | .listHorizontal{ | ||
+ | list-style: none; | ||
+ | display:inline-block; | ||
+ | margin-left: 0.3em; | ||
+ | } | ||
+ | |||
+ | .listHorizontal li{ | ||
+ | padding: 0px 5px 0px 5px; | ||
+ | } | ||
+ | |||
+ | div#globalWrapper { | ||
+ | position: relative; | ||
+ | font-size: 100%; | ||
+ | width: 100%; | ||
+ | margin: 0; | ||
+ | padding: 0; | ||
+ | padding-bottom: 10px; | ||
+ | } | ||
+ | |||
+ | </style> | ||
</head> | </head> |
Revision as of 02:04, 18 October 2014
NOTEBOOK
Immobilization Process
The electric charge of the nanocomposite is then developed negative due to various functional groups such as carboxylic acids, hydroxyl and epoxy groups (oxidized graphene) and sulfonic groups of polyaniline. Therefore, the immobilization of probe DNA was mediated by aluminum nitrate, which enabled the immobilization of the phosphate groups of the nanocomposite matrix. The modification of the electrode was initially performed with 100 ul of the solution of nanocomposite mixed with 50mL of 0.1M aluminum nitrate. Then, 50mL of the final solution was mixed with 50mL DNA probe and remained under stirring 300 rpm for 30 minutes. Finally, 5μl of the compound was added to the labor "drop-by-drop" technique for electrode and dried for 30 minutes in an oven at 50 ºC.