Team:LIKA-CESAR-Brasil/TestColialert

From 2014.igem.org

(Difference between revisions)
(Created page with "{{CSS/Main}} <html lang="pt-br"> <head> <meta charset="utf-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge"> <meta name="viewport" content="width=device-wid...")
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<link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.2.0/css/bootstrap.min.css">
<link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.2.0/css/bootstrap.min.css">
-
     <link href="https://dl.dropbox.com/s/3g6ibzuen1skq16/lika.css" rel="stylesheet">
+
     <style type="text/css">
 +
body {
 +
font-size:12px;
 +
color:#111;
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height:100%;
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background:#ededed;
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/*min-width: 1326px;*/
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}
 +
.container {
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width:80%;
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background: rgb(255,255,255); /* Old browsers */
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background: -moz-linear-gradient(top,  rgba(255,255,255,1) 0%, rgba(237,237,237,1) 100%); /* FF3.6+ */
 +
background: -webkit-gradient(linear, left top, left bottom, color-stop(0%,rgba(255,255,255,1)), color-stop(100%,rgba(237,237,237,1))); /* Chrome,Safari4+ */
 +
background: -webkit-linear-gradient(top,  rgba(255,255,255,1) 0%,rgba(237,237,237,1) 100%); /* Chrome10+,Safari5.1+ */
 +
background: -o-linear-gradient(top,  rgba(255,255,255,1) 0%,rgba(237,237,237,1) 100%); /* Opera 11.10+ */
 +
background: -ms-linear-gradient(top,  rgba(255,255,255,1) 0%,rgba(237,237,237,1) 100%); /* IE10+ */
 +
background: linear-gradient(to bottom,  rgba(255,255,255,1) 0%,rgba(237,237,237,1) 100%); /* W3C */
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filter: progid:DXImageTransform.Microsoft.gradient( startColorstr='#ffffff', endColorstr='#ededed',GradientType=0 ); /* IE6-9 */
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}
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.navbar-brand {
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padding: 5px;
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}
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.col-md-10  {
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width:100%;
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padding: 20px 70px 20px 50px;
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min-height:200px;
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padding-bottom:50px;
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}
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.col-md-4 {
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padding-bottom:70px;
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}
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.navbar-inverse {
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    background-color: #ededed;
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border-color: #ededed;
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}
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.navbar-inverse .navbar-nav > .active > a, .navbar-inverse .navbar-nav > .active > a:hover, .navbar-inverse .navbar-nav > .active > a:focus {
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    color: #006600;
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    background-color: #8cff00;
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}
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.navbar-inverse .navbar-nav > .open > a, .navbar-inverse .navbar-nav > .open > a:hover, .navbar-inverse .navbar-nav > .open > a:focus {
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    color: #006600;
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    background-color: #8cff00;
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}
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.navbar-inverse .navbar-nav > li > a:hover, .navbar-inverse .navbar-nav > li > a:focus {
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    color: #006600;
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    background-color: transparent;
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}
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.navbar-inverse .navbar-nav > li > a:focus {
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    color: #006600;
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    background-color: transparent;
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}
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.nav > li > a {
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    display: block;
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    padding: 25px 15px;
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    position: relative;
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}
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.menu{
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color:black;
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z-index:999;
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position:relative;
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float:right;
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margin:auto;
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}
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.videohome{
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background:#ededed;
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}
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.menu ul{
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margin:0 auto;
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}
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.jumbotron {
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background:url("https://static.igem.org/mediawiki/2014/7/7a/Banner_top.jpg");
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border-top: solid 5px #8cff00;
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border-bottom: solid 5px #8cff00;
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background-size:cover;
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width:80%;
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height:231px;
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margin:auto;
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margin-top:40px;
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}
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.jumbotron02 {
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background:url("https://static.igem.org/mediawiki/2014/5/52/Banner_top02.jpg");
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border-top: solid 5px #8cff00;
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border-bottom: solid 5px #8cff00;
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background-size:cover;
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width:80%;
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height:231px;
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margin:auto;
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margin-top:80px;
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}
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.jumbotronSafety {
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background:url("https://static.igem.org/mediawiki/2014/7/7a/Banner_top.jpg");
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border-top: solid 5px #8cff00;
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border-bottom: solid 5px #8cff00;
 +
background-size:cover;
 +
width:80%;
 +
height:231px;
 +
margin:auto;
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margin-top:80px;
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}
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.jumbotronParts {
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background:url("https://static.igem.org/mediawiki/2014/b/be/Banner_top04.jpg");
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border-top: solid 5px #8cff00;
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border-bottom: solid 5px #8cff00;
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background-size:cover;
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width:80%;
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height:231px;
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margin:auto;
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margin-top:80px;
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}
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.jumbotronHuman {
 +
background:url("https://static.igem.org/mediawiki/2014/1/12/Banner_top05.jpg");
 +
border-top: solid 5px #8cff00;
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border-bottom: solid 5px #8cff00;
 +
background-size:cover;
 +
width:80%;
 +
height:231px;
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margin:auto;
 +
margin-top:80px;
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}
 +
.jumbotronProject {
 +
background:url("https://static.igem.org/mediawiki/2014/b/be/Banner_top04.jpg");
 +
border-top: solid 5px #8cff00;
 +
border-bottom: solid 5px #8cff00;
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background-size:cover;
 +
width:80%;
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height:231px;
 +
margin:auto;
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margin-top:80px;
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}
 +
.jumbotronAdvisors {
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background:url("https://static.igem.org/mediawiki/2014/3/3b/Banner_top03.jpg");
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border-top: solid 5px #8cff00;
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border-bottom: solid 5px #8cff00;
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background-size:cover;
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width:80%;
 +
height:231px;
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margin:auto;
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margin-top:80px;
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}
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 +
.igem-logo{
 +
    background:url("https://static.igem.org/mediawiki/2014/4/4e/Banner_top_03.png") ;
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    background-color:transparent;
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    width: 50px;
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    height:50px;
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    z-index: 99;
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    background-repeat: no-repeat;
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}
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/*
 +
.navbar .container {
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min-width: 1322px;
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    z-index: 9999;
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}
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*/
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h2 {
 +
font-size: 26px;
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color:#339900;
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}
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.videocapa{
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width:35%;
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float:right;
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min-height:300px;
 +
margin-top:10px;
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}
 +
p {
 +
font-size: 12px;
 +
color:#444;
 +
}
 +
footer {
 +
background:#b8b8b8;
 +
width:100%;
 +
padding: 50px;
 +
  clear:both;
 +
position: relative;
 +
bottom:0;
 +
border-top: solid 8px #8cff00;
 +
}
 +
footer p {
 +
margin:auto;
 +
padding-top:10px;
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text-align:center;
 +
width:50%;
 +
color:#006600;
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}
 +
.firstHeading {
 +
display:none;
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}
 +
table{
 +
color:#888;
 +
font-size:16px;
 +
border: solid 1px #ccc;
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}
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h1 {
 +
font-size: 26px;
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color:#339900;
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}
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h2 {
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font-size: 22px;
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color:#339900;
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}
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h3 {
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font-size: 18px;
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color:#339900;
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}
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h4 {
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font-size: 16px;
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color:#339900;
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}
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 +
.listHorizontal{
 +
    list-style: none;
 +
    display:inline-block;
 +
    margin-left: 0.3em;
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}
 +
 
 +
.listHorizontal li{
 +
    padding: 0px 5px 0px 5px;
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}
 +
 
 +
div#globalWrapper {
 +
position: relative;
 +
font-size: 100%;
 +
width: 100%;
 +
margin: 0;
 +
padding: 0;
 +
padding-bottom: 10px;
 +
}
 +
 
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</style>
   </head>
   </head>

Revision as of 02:03, 18 October 2014

LIKA | CESAR

NOTEBOOK

Test of ColiAlert with different pH

  • 1) 200 µl of the bacterial suspension (cells with chromogenic protein) were mixed with 1 mL of acetic acid. Homogenized mixture to the tube changing color. After change of color was added to this tube 500 µl a solution of tri-basic. Mix until the solution color change. (Reversible by changing pH reaction).
  • 2) 200 µl of the bacterial suspension was mixed with 1 ml of lysis buffer. The solution was homogenized by observing color change. After change of color was added to 500 µl Tris base solution, homogenized again for observation of solution color change. (Reaction irreversible cell bacterial lysis and release intracellular proteins).
Marca LIKA Marca CESAR