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Team:OUC-China/Project Result
From 2014.igem.org
(Difference between revisions)
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<div id="mainContent" style="width:1024px;background-color:#d2e9fd;padding:10px;margin:0 auto"> | <div id="mainContent" style="width:1024px;background-color:#d2e9fd;padding:10px;margin:0 auto"> | ||
<h3 class="text-muted">mini plasmid & double plasmid system</h3> | <h3 class="text-muted">mini plasmid & double plasmid system</h3> | ||
| - | <h4>mini plasmid</h4> | + | <h4'''>mini plasmid'''</h4> |
<h4>We get the oriT region of plasmid RP4 by means of PCR, and ligate it with the reporter gene BBa_J04450, then a non-self-controllable conjugated part is constructed.</h4> | <h4>We get the oriT region of plasmid RP4 by means of PCR, and ligate it with the reporter gene BBa_J04450, then a non-self-controllable conjugated part is constructed.</h4> | ||
<img src="https://static.igem.org/mediawiki/2014/1/19/OUC-China_result_K1439001.jpg" /> | <img src="https://static.igem.org/mediawiki/2014/1/19/OUC-China_result_K1439001.jpg" /> | ||
<h4>In the meantime, we ligate BBa_J04450 with BBa_J01003 to test the conjugated ability of BBa_J01003.</h4> | <h4>In the meantime, we ligate BBa_J04450 with BBa_J01003 to test the conjugated ability of BBa_J01003.</h4> | ||
<img src="https://static.igem.org/mediawiki/2014/a/a4/OUC-China_result_K1439002.jpg" /> | <img src="https://static.igem.org/mediawiki/2014/a/a4/OUC-China_result_K1439002.jpg" /> | ||
| - | <h4>RP4 OriT 位点的失活</h4> | + | |
| + | <h4>'''RP4 OriT 位点的失活'''</h4> | ||
<h4>这一部分我们利用同源重组去失活RP4的OriT位点，很遗憾我们第一次的实验失败了，在比赛之前我们没有时间做第二次实验。在比赛结束后，我们会继续这部分的实验。但利用同源重组去失活OriT位点在理论上是完全行的通的。</h4> | <h4>这一部分我们利用同源重组去失活RP4的OriT位点，很遗憾我们第一次的实验失败了，在比赛之前我们没有时间做第二次实验。在比赛结束后，我们会继续这部分的实验。但利用同源重组去失活OriT位点在理论上是完全行的通的。</h4> | ||
Revision as of 08:42, 17 October 2014
mini plasmid & double plasmid system
We get the oriT region of plasmid RP4 by means of PCR, and ligate it with the reporter gene BBa_J04450, then a non-self-controllable conjugated part is constructed.
In the meantime, we ligate BBa_J04450 with BBa_J01003 to test the conjugated ability of BBa_J01003.
'''RP4 OriT 位点的失活'''
这一部分我们利用同源重组去失活RP4的OriT位点，很遗憾我们第一次的实验失败了，在比赛之前我们没有时间做第二次实验。在比赛结束后，我们会继续这部分的实验。但利用同源重组去失活OriT位点在理论上是完全行的通的。
