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Team:OUC-China/Project Result
From 2014.igem.org
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<h4>mini plasmid</h4> | <h4>mini plasmid</h4> | ||
<h4>We get the oriT region of plasmid RP4 by means of PCR, and ligate it with the reporter gene BBa_J04450, then a non-self-controllable conjugated part is constructed.</h4> | <h4>We get the oriT region of plasmid RP4 by means of PCR, and ligate it with the reporter gene BBa_J04450, then a non-self-controllable conjugated part is constructed.</h4> | ||
| - | + | <img src="https://static.igem.org/mediawiki/2014/1/19/OUC-China_result_K1439001.jpg" /> | |
| + | <h4>In the meantime, we ligate BBa_J04450 with BBa_J01003 to test the conjugated ability of BBa_J01003.</h4> | ||
| + | <img src="https://static.igem.org/mediawiki/2014/a/a4/OUC-China_result_K1439002.jpg" /> | ||
| + | <h4>RP4 OriT 位点的失活</h4> | ||
| + | <h4>这一部分我们利用同源重组去失活RP4的OriT位点，很遗憾我们第一次的实验失败了，在比赛之前我们没有时间做第二次实验。在比赛结束后，我们会继续这部分的实验。但利用同源重组去失活OriT位点在理论上是完全行的通的。</h4> | ||
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</div> | </div> | ||
<div id="childNavigator" style="width:180px"> | <div id="childNavigator" style="width:180px"> | ||
Revision as of 08:41, 17 October 2014
mini plasmid & double plasmid system
mini plasmid
We get the oriT region of plasmid RP4 by means of PCR, and ligate it with the reporter gene BBa_J04450, then a non-self-controllable conjugated part is constructed.
In the meantime, we ligate BBa_J04450 with BBa_J01003 to test the conjugated ability of BBa_J01003.
RP4 OriT 位点的失活
这一部分我们利用同源重组去失活RP4的OriT位点，很遗憾我们第一次的实验失败了，在比赛之前我们没有时间做第二次实验。在比赛结束后，我们会继续这部分的实验。但利用同源重组去失活OriT位点在理论上是完全行的通的。
