Team:Hong Kong HKUST/riboregulator/parts

From 2014.igem.org

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<div class='content_2'><h3><font color="white">Parts that we submitted</font></h3></div>
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<div class='content_2'><h3><font color="white">Parts that we designed and submitted</font></h3></div>
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<div class='content_2'><h3><font color="white">Parts that we are also characterizing</font></h3></div>
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<div class='content_2'><h3><font color="white">Parts that we constructed and submitted</font></h3></div>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J01080">BBa_J01080</a></td>
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  <td>Lock3</td>     
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  <td>Biobrick version of Isaacs' riboregulator cis repressed lock, crR12, and modified version of Riboregulator Lock 1.</td>  
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  <td>40</td>
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</tr>
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<tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J01086">BBa_J01086</a></td>
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  <td>Key3</td>     
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  <td>Biobricked version of Isaacs' riboregulator trans activating key, taR12, and modified version of Riboregulator Key 1.</td>  
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  <td>94</td>
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</tr>
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<tr>
<tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K175031">BBa_K175031</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K175031">BBa_K175031</a></td>  
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<br><br>
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<div class='content_2'><h3><font color="white">Parts that already exist in Parts Registry and are currently under characterization</font></h3></div>
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<table class= "catalog_table">
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<thead>
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<tr>
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<th scope="col">Name</th>
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<th scope="col">Type</th>
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<th scope="col">Description</th>
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<th scope="col">Length</th>
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</tr>
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</thead>
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<tbody>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J23031">BBa_J23031</a></td>
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  <td>Lock 3c</td>     
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  <td>lock3c</td>  
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  <td>42</td>
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</tr>
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<tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J23008">BBa_J23008</a></td>
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  <td>Key 3c</td>     
 +
  <td>Encodes a short RNA sequence that "unlocks" the biobrick part <a href="http://parts.igem.org/Part:BBa_J01122">J01122</a> (lock3) which is locked RFP.</td>  
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  <td>42</td>
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</tr>
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</tbody>
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</table>
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 +
<div class = "catalog_table_area">
 +
<div class='content_2'><h3><font color="white">Parts that we designed but did not submit</font></h3></div>
 +
<table class= "catalog_table">
 +
<thead>
 +
<tr>
 +
<th scope="col">Name</th>
 +
<th scope="col">Type</th>
 +
<th scope="col">Description</th>
 +
<th scope="col">Length</th>
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</tr>
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</thead>
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<tbody>
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<tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379021">BBa_K1379021</a></td>
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  <td>Key 1 Double Terminator</td>     
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  <td>Trans-Activator with terminator for lock 1</td>  
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  <td></td>
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</tr>
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<tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379023">BBa_K1379023</a></td>
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  <td>Key 3 double terminator</td>     
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  <td>Trans-Activator with terminator for lock 3</td>  
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  <td></td>
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</tr>
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<tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379025">BBa_K1379025</a></td>
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  <td>Key 3c double terminator</td>     
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  <td>Trans-Activator with terminator for lock 3C</td>  
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  <td></td>
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</tr>
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                                <tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379027">BBa_K1379027</a></td>
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  <td>MTA double terminator</td>     
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  <td>Trans-Activator with terminator for MCR</td>  
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  <td></td>
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</tr>
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                                <tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379029">BBa_K1379029</a></td>
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  <td>HKUST Key 1 double terminator</td>     
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  <td>Trans-Activator with terminator for HKUST Lock 1</td>  
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  <td></td>
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</tr>
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                                <tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379030">BBa_K1379030</a></td>
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  <td>Cis-Repressing device 1</td>     
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  <td>Constitutive promoted, Cis-repressed GFP Coding device Lock 1</td>  
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  <td></td>
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</tr>
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                                <tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379031">BBa_K1379031</a></td>
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  <td>AraC Key 1</td>     
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  <td>AraC inducible Trans-Activator device, Key 1</td>  
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  <td></td>
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</tr>
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                                <tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379032">BBa_K1379032</a></td>
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  <td>Cis-Repressing device 3</td>     
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  <td>Constitutive promoted, Cis-repressed GFP Coding device Lock 3</td>  
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  <td></td>
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</tr>
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                                <tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379033">BBa_K1379033</a></td>
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  <td>AraC Key 3</td>     
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  <td>AraC inducible Trans-Activator device, Key 3</td>  
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  <td></td>
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</tr>
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                                <tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379034">BBa_K1379034</a></td>
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  <td>Cis-Repressing device 3C</td>     
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  <td>Constitutive promoted, Cis-repressed GFP Coding device Lock 3C</td>  
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  <td></td>
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</tr>
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                                <tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379035">BBa_K1379035</a></td>
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  <td>AraC Key 3C</td>     
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  <td>AraC inducible Trans-Activator device for, Key 3C</td>  
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  <td></td>
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</tr>
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                                <tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379036">BBa_K1379036</a></td>
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  <td>Cis-Repressing device MCR</td>     
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  <td>Constitutive promoted, Cis-represed GFP Coding device Lock MCR</td>  
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  <td></td>
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</tr>
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                                <tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379037">BBa_K1379037</a></td>
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  <td>AraC MTA</td>     
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  <td>AraC inducible Trans-Activator device, MTA</td>  
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  <td></td>
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</tr>
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                                <tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379038">BBa_K1379038</a></td>
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  <td>Cis-Repressing device HKUST 1</td>     
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  <td>Constitutive promoted, Cis-repressed GFP Coding device HKUST Lock 1</td>  
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  <td></td>
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</tr>
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                                <tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379039">BBa_K1379039</a></td>
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  <td>AraC HKUST Key 1</td>     
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  <td>AraC inducible Trans-Activator device, HKUST Key 1</td>  
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  <td></td>
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</tr>
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                                <tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379040">BBa_K1379040</a></td>
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  <td>Repression device 1</td>     
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  <td>Repression measurement device 1</td>  
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  <td></td>
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</tr>
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                                <tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379041">BBa_K1379041</a></td>
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  <td>Repression device 3</td>     
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  <td>Repression measurement device 3</td>  
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  <td></td>
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</tr>
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                                <tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379042">BBa_K1379042</a></td>
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  <td>Repression device 3C</td>     
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  <td>Repression measurement device 3C</td>  
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  <td></td>
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</tr>
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                                <tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379043">BBa_K1379043</a></td>
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  <td>Repression device Medium</td>     
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  <td>Repression measurement device Medium</td>  
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  <td></td>
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</tr>
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                                <tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379044">BBa_K1379044</a></td>
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  <td>Cis-Repression device HKUST 1</td>     
 +
  <td>Repression measurement device HKUST 1</td>  
 +
  <td></td>
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</tr>
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 +
                                <tr>
 +
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379049">BBa_K1379049</a></td>
 +
  <td>Cis repressed GFP lock 1, with Trans-activator key 1</td>     
 +
  <td>Cis repressed GFP lock 1, with Trans-activator key 1</td>  
 +
  <td></td>
 +
</tr>
 +
 +
</table>
</div>
</div>

Revision as of 15:58, 17 October 2014



Riboregulator Parts

Parts that we designed and submitted

Name Type Description Length
BBa_K1379015 Lock 3C RBS B0032 derivate for Lock 3C 12
BBa_K1379018 HKUST Lock 1 Cis-Repressor HKUST Lock 1 45
BBa_K1379019 HKUST Key 1 Trans-Activator for HKUST Key 1 82
BBa_K1379020 Cis-repressed GFP Lock 1 Cis-repressed GFP Generator Lock 1 903
BBa_K1379022 Cis-repressed GFP Generator Lock 3 Cis-repressed GFP Generator Lock 3 905
BBa_K1379024 Cis-repressed GFP Lock 3C Cis-repressed GFP Generator Lock 3C 905
BBa_K1379026 Cis-repressed GFP Lock MCR Cis-repressed GFP Generator MCR 910
BBa_K1379028 Cis-repressed GFP HKUST Lock 1 Cis-repressed GFP Generator HKUST Lock 1 908
BBa_K1379046 Lock 1 RBS GFP generator GFP generator using a Lock 1 modified RBS 873
BBa_K1379047 Lock 3C RBS GFP generator GFP generator using a Lock 3C modified RBS 875


Parts that we constructed and submitted

Name Type Description Length
BBa_I0500 Inducible pBad/araC promoter Inducible pBad/araC promoter 1210
BBa_J01010 Riboregulator Lock 1 Biobrick version of Isaacs' riboregulator cis repressed lock, crR12. 40
BBa_J01080 Lock3 Biobrick version of Isaacs' riboregulator cis repressed lock, crR12, and modified version of Riboregulator Lock 1. 40
BBa_J01086 Key3 Biobricked version of Isaacs' riboregulator trans activating key, taR12, and modified version of Riboregulator Key 1. 94
BBa_K175031 Lock for medium RBS (B0032) from the lock/key library TUD09 This biobrick generates a mRNA which forms a secondary structure blocking the (medium) RBS. 47
BBa_K175032 Key for lock of medium (K175031) RBS (B0032) from the lock/key library TUD09 This biobrick generates a mRNA which forms a secondary structure that opens LMR (K175031). 84
BBa_J23080 B0034 derived RBS to match spacing of J01122 RBS spacing modification to [RBS] such that the spacing between the shine-delgarno and the ORF is the same as it is in J01122. 10
BBa_K1379048 Cis repressed GFP, with Trans-activator Repression measurement device 1 with trans activator 2403


Parts that already exist in Parts Registry and are currently under characterization

Name Type Description Length
BBa_J23031 Lock 3c lock3c 42
BBa_J23008 Key 3c Encodes a short RNA sequence that "unlocks" the biobrick part J01122 (lock3) which is locked RFP. 42

Parts that we designed but did not submit

Name Type Description Length
BBa_K1379021 Key 1 Double Terminator Trans-Activator with terminator for lock 1
BBa_K1379023 Key 3 double terminator Trans-Activator with terminator for lock 3
BBa_K1379025 Key 3c double terminator Trans-Activator with terminator for lock 3C
BBa_K1379027 MTA double terminator Trans-Activator with terminator for MCR
BBa_K1379029 HKUST Key 1 double terminator Trans-Activator with terminator for HKUST Lock 1
BBa_K1379030 Cis-Repressing device 1 Constitutive promoted, Cis-repressed GFP Coding device Lock 1
BBa_K1379031 AraC Key 1 AraC inducible Trans-Activator device, Key 1
BBa_K1379032 Cis-Repressing device 3 Constitutive promoted, Cis-repressed GFP Coding device Lock 3
BBa_K1379033 AraC Key 3 AraC inducible Trans-Activator device, Key 3
BBa_K1379034 Cis-Repressing device 3C Constitutive promoted, Cis-repressed GFP Coding device Lock 3C
BBa_K1379035 AraC Key 3C AraC inducible Trans-Activator device for, Key 3C
BBa_K1379036 Cis-Repressing device MCR Constitutive promoted, Cis-represed GFP Coding device Lock MCR
BBa_K1379037 AraC MTA AraC inducible Trans-Activator device, MTA
BBa_K1379038 Cis-Repressing device HKUST 1 Constitutive promoted, Cis-repressed GFP Coding device HKUST Lock 1
BBa_K1379039 AraC HKUST Key 1 AraC inducible Trans-Activator device, HKUST Key 1
BBa_K1379040 Repression device 1 Repression measurement device 1
BBa_K1379041 Repression device 3 Repression measurement device 3
BBa_K1379042 Repression device 3C Repression measurement device 3C
BBa_K1379043 Repression device Medium Repression measurement device Medium
BBa_K1379044 Cis-Repression device HKUST 1 Repression measurement device HKUST 1
BBa_K1379049 Cis repressed GFP lock 1, with Trans-activator key 1 Cis repressed GFP lock 1, with Trans-activator key 1

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