Team:SF Bay Area DIYbio/Notebook

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<p><b><font size="5">Notebook</font></b></p><br>
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<p><b><font size="4">General Protocols</font></b></p>
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<p>
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</p><ul> 
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  <li><a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/ElectraCloningProtocol"> Electra Cloning Protocol </a><br>
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  </li><li><a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/DNAhandling"> DNA Handling Protocol </a><br>
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  </li><li>Media:Zymo E. coli competent cells transformation protocol.pdf<br>
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  </li><li>Media:D4025i.pdf (Zyppy plasmid midiprep protocol)<br>
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  </li><li>Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf <br>
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  </li><li>Media:Sequencing_Analysis_using_Serial_Cloner_2_16Oct2014.pdf‎<br></li></ul>
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<br>
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<p>General Documentation Information</p><ul>
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    <li><a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/NotebookDocumentation">Real Vegan Cheese Notebook Documentation Guidelines</a><br>
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    </li></ul><br>
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<p><b><font size="4">Experiments</font></b></p>
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<p><b>June 2014</b></p><ul>
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    <li><a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/NoteBookJune1">CR_062214_humanKappaCasein_001</a> <br></li></ul><p></p><br>
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<p><b>August 2014</b></p><ul>
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    <li><a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/AugustCloning">CLONE_082514_humKappaCasein(Kex+)</a><br>
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    </li><li><a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/AugustGrowthCurve">Growth Curve Experiment</a><br></li></ul><p></p><br>
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<p><b>September 2014</b></p><ul>
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    <li><a href="https://2014.igem.org/cloning7caesin">Cloning 7 casein gBlocks, <b>12 Sep 2014</b></a><br>
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    </li><li><a href="https://2014.igem.org/troubleshooting12.9">Troubleshooting failed transformation of P.FAKS.humBeta.S on <b>12 Sept. 2014</b></a><br>
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    </li><li><a href="https://2014.igem.org/CloningBovineBetaCaesinSept">Cloning bovine beta casein, Fam20C (Kex + &amp; -), from <b>27Sep2014</b></a><br>
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    </li><li><a href="https://2014.igem.org/Submitting10CaesinSept">Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing <b>30Sep2014</b></a><br></li></ul><p></p><br>
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<p><b>October 2014</b></p><ul>
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    <li><a href="https://2014.igem.org/InitialYeastStudyOct">Initial studies of yeast strains' growth conditions and troubleshooting <b>Oct.2</b></a><br>
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    </li><li><a href="https://2014.igem.org/RepeadMidiprepsOct">Repeat Midipreps to select new clones for sequencing for failed sequences from <b>Oct. 2</b></a><br>
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    </li><li><a href="https://2014.igem.org/CloningHumanAlphaS1Oct">Cloning human alpha casein S1, <b>04Oct2014</b></a><br>
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    </li><li><a href="https://2014.igem.org/CloningBovineKappaBetaOct">Cloning bovineKappa and bovineBeta into pSB1C3 Biobrick using NEB biobrick kit <b>08Oct2014</b></a><br>
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    </li><li><a href="https://2014.igem.org/SubmittingPlasmidSequencingOct">Submitting 4 repeat and 1 new casein in pD1214 plasmid for sequencing <b>10Oct2014</b></a><br>
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    </li><li><a href="https://2014.igem.org/CloningBovineKappaBetaBioBrickOct">Cloning bovineKappa and bovineBeta into pSB1C3 Biobrick using NEB biobrick kit <b>10Oct2014</b></a><br>
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    </li><li><a href="https://2014.igem.org/CloningBovineBetaOct">Cloning bovineBeta into pSB1C3 Biobrick using NEB biobrick kit <b>13Oct2014</b></a><br>
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    </li><li><a href="https://2014.igem.org/SubmittingFam20COct">Submitting FAM20C (Kex + &amp; Kex -) for sequencing with new internal primers <b>14Oct2014</b></a><br>
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    </li><li><a href="https://2014.igem.org/SubmittingFam20CkexOct">Submitting FAM20C Kex + clone 9A and bovineKappa and bovineBeta: pSB1C3 Biobrick for sequencing <b>15Oct2014</b></a><br><br></li></ul><p></p>
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Revision as of 05:36, 17 October 2014



Click here to edit this page!

Notebook


General Protocols

  • Electra Cloning Protocol
  • DNA Handling Protocol
  • Media:Zymo E. coli competent cells transformation protocol.pdf
  • Media:D4025i.pdf (Zyppy plasmid midiprep protocol)
  • Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf
  • Media:Sequencing_Analysis_using_Serial_Cloner_2_16Oct2014.pdf‎

General Documentation Information


Experiments

June 2014


August 2014


September 2014


October 2014