Team:AHUT China/Notebook
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Latest revision as of 04:09, 17 October 2014
A man can fail many times, but he isn't a failure until he begins to blame somebody else.
---J. Burroughs. Averican naturalist
Taq Plus PCR
MasterMix
0.1 U Taq Plus Polymerase/μl
500 μM dNTP each
20 mM Tris-HCl (pH8.3)
100 mM KCl
3 mM MgCl2
TIANgel Midi Purification Kit
DNA MW Standard Marker
LB media
1L LB medium | 1% |
Yeast Extract | 0.5% |
aCl | 1% |
E.coli
DH5a
Top10
Plasmids
pUC57:http://www.ncbi.nlm.nih.gov/nuccore/Y14837.1
PSB1C3
DNA Ligation Kit
Enzyme Cutting