Team:AHUT China/Notebook
From 2014.igem.org
A man can fail many times, but he isn't a failure until he begins to blame somebody else.
---J. Burroughs. Averican naturalist
![](https://static.igem.org/mediawiki/2014/2/22/AHUT_CHINA_2014_triangle.png)
Taq Plus PCR
MasterMix
0.1 U Taq Plus Polymerase/μl
500 μM dNTP each
20 mM Tris-HCl (pH8.3)
100 mM KCl
3 mM MgCl2
TIANgel Midi Purification Kit
![](https://static.igem.org/mediawiki/2014/3/31/AHUT_CHINA_2014_notebook_pic_4.jpg)
DNA MW Standard Marker
![](https://static.igem.org/mediawiki/2014/1/16/AHUT_CHINA_2014_notebook_pic_5.png)
LB media
1L LB medium | 1% |
Yeast Extract | 0.5% |
aCl | 1% |
E.coli
DH5a
Top10
Plasmids
![](https://static.igem.org/mediawiki/2014/f/f4/AHUT_CHINA_2014_notebook_pic_6.jpg)
pUC57:http://www.ncbi.nlm.nih.gov/nuccore/Y14837.1
PSB1C3
DNA Ligation Kit
![](https://static.igem.org/mediawiki/2014/2/26/AHUT_CHINA_2014_notebook_pic_7.jpg)
Enzyme Cutting
![](https://static.igem.org/mediawiki/2014/5/50/AHUT_CHINA_2014_notebook_pic_8.jpg)