Team:AHUT China/Notebook

From 2014.igem.org

A man can fail many times, but he isn't a failure until he begins to blame somebody else.

---J. Burroughs. Averican naturalist

NOTEBOOK

PROTOCOLS

Taq Plus PCR

MasterMix
0.1 U Taq Plus Polymerase/μl
500 μM dNTP each
20 mM Tris-HCl (pH8.3)
100 mM KCl
3 mM MgCl2

TIANgel Midi Purification Kit

DNA MW Standard Marker

LB media

1L LB medium	1%
Yeast Extract	0.5%
aCl	1%

E.coli

DH5a
Top10

Plasmids

pUC57:http://www.ncbi.nlm.nih.gov/nuccore/Y14837.1

PSB1C3

DNA Ligation Kit

Enzyme Cutting

Lab Pictures

- TEAM
- Members
- Instructors
- Attributions
- Acknowledgement
- PROJECT
- Background
- Design
- Results&future work
- Parts
- HUMAN PRACTICES
- Military Training
- Society
- APP and Sina Weibo
- Help Each Other
- School qualify
- SAFETY
- Safety
- NOTEBOOK
- Notebook
- Protocols
- Lab Pictures

Anhui University of Technology is located in the national civilized city, Nanjing metropolitan core cities - Ma'anshan City, Anhui Province. Anhui University of Technology is an engineering, metallurgical features, engineering, economics, management, arts, science, law and other disciplines coordinated development of key construction in Anhui multidisciplinary university, with "The Master, Bo" complete the multi-level education training system.