<!--TYPO3SEARCH_begin--><div id="c85" class="csc-default"><div><p><b>Short explanation:</b></p></div><div><p>Antarctic Phosphatase catalyzes the removal of 5' phosphate groups of DNA and RNA and thus prevents religation of cut vectors. It is used before ligation.</p></div><div></div><div><p><b>Procedure:</b></p></div><div><p>The reaction mix contains:</p></div><div><p>- up to 5 µg of digested DNA (no purification needed)</p></div><div><p>- 1/10 of reaction volume of 10x Antarctic Phosphatase Reaction Buffer</p></div><div><p>- 1 µL of Antarctic Phosphatase</p></div><div></div><div><p>- Incubate at 37° C for 30 minutes (3' ends) or 60 minutes (5' ends)</p></div><div><p>- Heat inactivate at 65° C for 15 minutes</p></div><div><p>- Continue with ligation</p></div></div><!--TYPO3SEARCH_end-->