Team:The Tech Museum/Notebook

From 2014.igem.org

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<td>1</td>
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<td>Promoter - RBS Pairs</td>
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<td>BBa_J23117 - BBa_J61112</td>
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<td>Sequence</td>
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<td>TTGACAGCTAGCTCAGTCCTAGGGATTGTGCTAGCCAATCTCTAGAGAAAGAGGTGACATAC</td>
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<td>2</td>
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<td>Promoter - RBS Pairs</td>
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<td>BBa_J23104 - BBa_J61107</td>
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<td>Sequence</td>
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<td>TTGACAGCTAGCTCAGTCCTAGGTATTGTGCTAGCTTACGTCTAGAGAAAGAAGAGACTCAC</td>
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<td></td>
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<td>3</td>
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<td>Promoter - RBS Pairs</td>
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<td>apFAB78 - apFAB954</td>
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<td>Sequence</td>
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<td>TTGACATTTATCCCTTGCGGCGATAGATTTAACGTATGACGGATCTTAATCTAGCTCAGGACAATTT</td>
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<td></td>
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<td>4</td>
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<td>Promoter - RBS Pairs</td>
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<td>apFAB76 - apFAB927</td>
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<td>Sequence</td>
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<td>TTGACATTTATCCCTTGCGGCGATATAATAGATATCTTAATCTAGCCCGGGAGTTTTTTCATTCCGGATCTTAATCTAGCTGGGGACTGTTT</td>
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<td></td>
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<td>5</td>
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<td>Promoter - RBS Pairs</td>
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<td>apFAB70 - apFAB844</td>
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<td>Sequence</td>
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<td>TTGACATCGCATCTTTTTGTACCTATAATGTGTGGATAGAGTATCTTAATCTAGCAGGGGACACTTT</td>
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<td></td>
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<td>6</td>
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<td>Promoter - RBS Pairs</td>
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<td>BBa_J23104 - apFAB909</td>
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<td>Sequence</td>
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<td>TTGACAGCTAGCTCAGTCCTAGGTATTGTGCTAGCTTACGATCTTAATCTAGCGAAGGATAGTTT</td>
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<td></td>
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<td>7</td>
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<td>Promoter - RBS Pairs</td>
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<td>apFAB45 - apFAB901</td>
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<td>Sequence</td>
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<td>AAAAAGAGTATTGACTTCGCATCTTTTTGTACCTATAATGTGTGGATAGCGG</td>
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</tr>
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<td></td>
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<td>8</td>
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<td>Promoter - RBS Pairs</td>
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<td>apFAB92 - apFAB863</td>
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<td>Sequence</td>
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<td>AAAAAATTTATTTGCTTTCAGGAAAATTTTTCTGCATAATTATTTCATGGAGCATCTTAATCTAGCGGGGGAGCGTTT</td>
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</tr>
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<td></td>
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<td>9</td>
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<td>Promoter - RBS Pairs</td>
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<td>apFAB71 - salis-4-10</td>
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<td>Sequence</td>
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<td>TTGACATCGCATCTTTTTGTACCTATAATAGATTCATGATGAAATCTCTTTTATCAAATATAAGCAGGAT</td>
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</table>
</table>

Revision as of 18:41, 16 October 2014

Home Team Project Notebook Community Engagement Attributions

Notebook

Timeline of major events:
May: Basic concept research and design
June: Software development initiated; plasmid design started and promoter-RBS’s picked
July: Wetlab testing of possible color protein combinations
August 10: Design of complete tri-color plasmids finalized
August 24: Assembly of tri-color plasmid pools completed (DNA2.0)
September 10: Tri-color plasmids optimized on museum wetlab setup
September 29: Software fully integrated with hardware on mobile exhibit
September 30 - October 3: Prototyping of mobile exhibit with museum staff and visitors
October 6 - October 17: Data collection on the museum floor with visitor!

Biology Details

Design of tri-color plasmid pool (June 2014)
9 promoter-rbs pairs of varying strength from Kosuri et al paper (2013):

Promoter - RBS Pairs Sequence
1 BBa_J23117 - BBa_J61112 TTGACAGCTAGCTCAGTCCTAGGGATTGTGCTAGCCAATCTCTAGAGAAAGAGGTGACATAC
2 BBa_J23104 - BBa_J61107 TTGACAGCTAGCTCAGTCCTAGGTATTGTGCTAGCTTACGTCTAGAGAAAGAAGAGACTCAC
3 apFAB78 - apFAB954 TTGACATTTATCCCTTGCGGCGATAGATTTAACGTATGACGGATCTTAATCTAGCTCAGGACAATTT
4 apFAB76 - apFAB927 TTGACATTTATCCCTTGCGGCGATATAATAGATATCTTAATCTAGCCCGGGAGTTTTTTCATTCCGGATCTTAATCTAGCTGGGGACTGTTT
5 apFAB70 - apFAB844 TTGACATCGCATCTTTTTGTACCTATAATGTGTGGATAGAGTATCTTAATCTAGCAGGGGACACTTT
6 BBa_J23104 - apFAB909 TTGACAGCTAGCTCAGTCCTAGGTATTGTGCTAGCTTACGATCTTAATCTAGCGAAGGATAGTTT
7 apFAB45 - apFAB901 AAAAAGAGTATTGACTTCGCATCTTTTTGTACCTATAATGTGTGGATAGCGG
8 apFAB92 - apFAB863 AAAAAATTTATTTGCTTTCAGGAAAATTTTTCTGCATAATTATTTCATGGAGCATCTTAATCTAGCGGGGGAGCGTTT
9 apFAB71 - salis-4-10 TTGACATCGCATCTTTTTGTACCTATAATAGATTCATGATGAAATCTCTTTTATCAAATATAAGCAGGAT

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