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Team:EPF Lausanne/Data
From 2014.igem.org
(Difference between revisions)
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<p id="doc_and_sub">Documented & submitted: | <p id="doc_and_sub">Documented & submitted: | ||
<ul> | <ul> | ||
| - | <li> | + | <li>CpxR reporters were made with the promoter in forward and reverse direction, respectively <a target="_blank" href="http://parts.igem.org/Part:BBa_K1486049">BBa_K1486049</a> and <a target="_blank" href="http://parts.igem.org/Part:BBa_K1486050">BBa_K1486050</a>.</li> |
| - | <li> | + | <li>A BioBrick consisting of <a target="_blank" href="http://parts.igem.org/Part:BBa_K1486001">CpxR under Arabinose Promoter</a> was made, and then sfGFP was fused to CpxR's N terminus (<a target="_blank" href="http://parts.igem.org/Part:BBa_K1486002">BBa_K1486002</a>) and C terminus (<a target="_blank" href="http://parts.igem.org/Part:BBa_K1486005">BBa_K1486005</a>).</li> |
| - | < | + | |
<li>BBa_K1486008 (CxpR & Split IFP1.4 [Cterm + Cterm] http://parts.igem.org/Part:BBa_K1486002 )</li> | <li>BBa_K1486008 (CxpR & Split IFP1.4 [Cterm + Cterm] http://parts.igem.org/Part:BBa_K1486002 )</li> | ||
<li>BBa_K1486043 (LeuZ + rLuc http://parts.igem.org/Part:BBa_K1486043 ) </li> | <li>BBa_K1486043 (LeuZ + rLuc http://parts.igem.org/Part:BBa_K1486043 ) </li> | ||
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<p id="works">Works as expected: | <p id="works">Works as expected: | ||
<ul> | <ul> | ||
| - | |||
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<li>BBa_K1486008 (CxpR & Split IFP1.4 [Cterm + Cterm] http://parts.igem.org/Part:BBa_K1486008 )</li> | <li>BBa_K1486008 (CxpR & Split IFP1.4 [Cterm + Cterm] http://parts.igem.org/Part:BBa_K1486008 )</li> | ||
<li>BBa_K1486012 (CpxR IFP1 http://parts.igem.org/Part:BBa_K1486012 )</li> | <li>BBa_K1486012 (CpxR IFP1 http://parts.igem.org/Part:BBa_K1486012 )</li> | ||
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<li>BBa_K1486034 (R.reniformis luciferase + ADH1 terminator + CaUra3 http://parts.igem.org/Part:BBa_K1486034 )</li> | <li>BBa_K1486034 (R.reniformis luciferase + ADH1 terminator + CaUra3 http://parts.igem.org/Part:BBa_K1486034 )</li> | ||
<li>BBa_K1486035 (sfGFPC + ADH1 terminator + CaUra3 Cassette http://parts.igem.org/Part:BBa_K1486035 )</li> | <li>BBa_K1486035 (sfGFPC + ADH1 terminator + CaUra3 Cassette http://parts.igem.org/Part:BBa_K1486035 )</li> | ||
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</ul></p> | </ul></p> | ||
<br /> | <br /> | ||
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<p> | <p> | ||
<ul> | <ul> | ||
| - | <li>We realized that <a target="_blank" href="http://parts.igem.org/Part:BBa_K339007">Calgary's CpxR reporter biobrick</a> was missing a part of the sequence, so we repaired it and sent it as our <a target="_blank" href="http://parts.igem.org/Part:BBa_K1486048">BBa_K1486048</a>. The BioBrick was also perfected by testing the complete CpxR target (as Calgary's part did not include the whole sequence). | + | <li>We realized that <a target="_blank" href="http://parts.igem.org/Part:BBa_K339007">Calgary's CpxR reporter biobrick</a> was missing a part of the sequence, so we repaired it and sent it as our <a target="_blank" href="http://parts.igem.org/Part:BBa_K1486048">BBa_K1486048</a>. The BioBrick was also perfected by testing the complete CpxR target (as Calgary's part did not include the whole sequence). These are BioBricks <a target="_blank" href="http://parts.igem.org/Part:BBa_K1486049">BBa_K1486049</a> and <a target="_blank" href="http://parts.igem.org/Part:BBa_K1486050">BBa_K1486050</a>, with the promoter in forward and reverse direction respectively.</li> |
| + | |||
<li>Submitted the two parts of the split of <a href="http://parts.igem.org/Part:BBa_K325108">EPIC Firefly luciferase</a> (N-terminal part (<a href="http://parts.igem.org/Part:BBa_K1486016">BBa_K1486016</a>) and C-terminal part (<a href="http://parts.igem.org/Part:BBa_K1486017">BBa_K1486017</a>)) from Cambridge 2010. The plasmid (<a href="http://parts.igem.org/Part:BBa_K1486018">BBa_K1486018</a>) containing the two parts of the split separated by a spacer can be very useful as a negative control or to establish a background noise for a complementation assay experiment.</li> | <li>Submitted the two parts of the split of <a href="http://parts.igem.org/Part:BBa_K325108">EPIC Firefly luciferase</a> (N-terminal part (<a href="http://parts.igem.org/Part:BBa_K1486016">BBa_K1486016</a>) and C-terminal part (<a href="http://parts.igem.org/Part:BBa_K1486017">BBa_K1486017</a>)) from Cambridge 2010. The plasmid (<a href="http://parts.igem.org/Part:BBa_K1486018">BBa_K1486018</a>) containing the two parts of the split separated by a spacer can be very useful as a negative control or to establish a background noise for a complementation assay experiment.</li> | ||
<li>Compared the <a href="http://parts.igem.org/Part:BBa_K325108">EPIC Firefly luciferase</a> from Cambridge 2010 team to the renilla luciferase (<a href="http://parts.igem.org/Part:BBa_K1486022">BBa_K1486022</a>) in the same conditions, to determine which one is best suited for a complementation assay experiment. The full and split luciferases has been compared. Renilla luciferase (full and splits(<a href="http://parts.igem.org/Part:BBa_K1486021">BBa_K1486021</a>)) have been submitted.</li> | <li>Compared the <a href="http://parts.igem.org/Part:BBa_K325108">EPIC Firefly luciferase</a> from Cambridge 2010 team to the renilla luciferase (<a href="http://parts.igem.org/Part:BBa_K1486022">BBa_K1486022</a>) in the same conditions, to determine which one is best suited for a complementation assay experiment. The full and split luciferases has been compared. Renilla luciferase (full and splits(<a href="http://parts.igem.org/Part:BBa_K1486021">BBa_K1486021</a>)) have been submitted.</li> | ||
Revision as of 18:23, 16 October 2014
DATA
Data For Our Favorite New Parts
Characterization of New Parts Submitted to the Registry
Documented & submitted:
- CpxR reporters were made with the promoter in forward and reverse direction, respectively BBa_K1486049 and BBa_K1486050.
- A BioBrick consisting of CpxR under Arabinose Promoter was made, and then sfGFP was fused to CpxR's N terminus (BBa_K1486002) and C terminus (BBa_K1486005).
- BBa_K1486008 (CxpR & Split IFP1.4 [Cterm + Cterm] http://parts.igem.org/Part:BBa_K1486002 )
- BBa_K1486043 (LeuZ + rLuc http://parts.igem.org/Part:BBa_K1486043 )
- BBa_K1486056 (Ga1Mut http:/parts.igem.org/Part:BBa_K1486056 )
Works as expected:
- BBa_K1486008 (CxpR & Split IFP1.4 [Cterm + Cterm] http://parts.igem.org/Part:BBa_K1486008 )
- BBa_K1486012 (CpxR IFP1 http://parts.igem.org/Part:BBa_K1486012 )
- BBa_K1486013 (cpxR IFP2 http://parts.igem.org/Part:BBa_K1486013 )
- BBa_K1486014 (IFP1 CpxR rLuc http://parts.igem.org/Part:BBa_K1486014 )
- BBa_K1486015 (IFP2 CpxR rLuc http://parts.igem.org/Part:BBa_K1486015 )
- BBa_K1486023 (Yeast optimized surperfolder GFP http://parts.igem.org/Part:BBa_K1486023 )
- BBa_K1486024 (Yeast Kanamycin resistance http://parts.igem.org/Part:BBa_K1486024 )
- BBa_K1486025 (ADH1 Terminator http://parts.igem.org/Part:BBa_K1486025 )
- BBa_K1486026 (sfGFP + AHD1 terminator + Kanamycin resistance for yeast http://parts.igem.org/Part:BBa_K1486026 )
- BBa_K1486027 (R.reniformis luciferase + ADH1 terminator + Kanamycin resistance http://parts.igem.org/Part:BBa_K1486027 )
- BBa_K1486028 (Yeast optimized sfGFP N-terminus (1-214) http://parts.igem.org/Part:BBa_K1486028 )
- BBa_K1486029 (sfGFPN + ADH1 terminator + Kanamycin resistance http://parts.igem.org/Part:BBa_K1486029)
- BBa_K1486031 (CaUra3 selection marker http://parts.igem.org/Part:BBa_K1486031)
- BBa_K1486034 (R.reniformis luciferase + ADH1 terminator + CaUra3 http://parts.igem.org/Part:BBa_K1486034 )
- BBa_K1486035 (sfGFPC + ADH1 terminator + CaUra3 Cassette http://parts.igem.org/Part:BBa_K1486035 )
Further Characterization and Improvement of Parts Already in the Registry
- We realized that Calgary's CpxR reporter biobrick was missing a part of the sequence, so we repaired it and sent it as our BBa_K1486048. The BioBrick was also perfected by testing the complete CpxR target (as Calgary's part did not include the whole sequence). These are BioBricks BBa_K1486049 and BBa_K1486050, with the promoter in forward and reverse direction respectively.
- Submitted the two parts of the split of EPIC Firefly luciferase (N-terminal part (BBa_K1486016) and C-terminal part (BBa_K1486017)) from Cambridge 2010. The plasmid (BBa_K1486018) containing the two parts of the split separated by a spacer can be very useful as a negative control or to establish a background noise for a complementation assay experiment.
- Compared the EPIC Firefly luciferase from Cambridge 2010 team to the renilla luciferase (BBa_K1486022) in the same conditions, to determine which one is best suited for a complementation assay experiment. The full and split luciferases has been compared. Renilla luciferase (full and splits(BBa_K1486021)) have been submitted.
Microfluidics
- Design of SmashColi - a testing chip to analyse the effects of different mechanical stresses on cells
- Design of FilterColi - a testing chip to analyse the effects of different osmotic stresses on cells
- Design of the BioPad - a large-scaled chip implemented to be the touch-senstive interface of our final trackpad
- Design of CleanColi - blabla
| MITOMI | MITOMI modified | SmashColi | BioPad | FilterColi | CleanColi | |
|---|---|---|---|---|---|---|
| Full chip |  |
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| Unit Cell |  |
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| Designed | ||||||
| Mold fabrication | ||||||
| Fabrication of the chip | ||||||
| Application | ||||||
| Reference | MITOMI paper |
Human Practices
- Met with a journalist from the biggest newspaper of our region (Le Temps) and got an article about our project.
- Our work was commented by Bent Stumpe, inventor of the touchscreen, as well as Rolf Heuer, the current director of the CERN, in Geneva.
- Organized an outreach event with 80 highschool students at EPFL, teaching them about synthetic biology as well as laboratory techniques and made them participate in a game called « mini iGEM ».
- Presented our work at the Hackuarium in Renens.
Sponsors
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