Team:TU Darmstadt/Notebook/Materials/Primers

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<p>Sie sind hier:&nbsp; <a href="https://2014.igem.org/Team:TU_Darmstadt/" >wiki</a> &rsaquo;&nbsp;<a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook" >Notebook</a> &rsaquo;&nbsp;<span class="current"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials" >Materials </a> &rsaquo;&nbsp;<span class="current"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Primers"> Primers </a></span></p>
 
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<ul class="menu"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt/" >Home</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Project" >Project</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results" >Results</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/PolicyandPractices" >Policy & Practices</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Achievements" >Achievements</a></li><li class="active"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook" >Notebook</a><ul class="menu2"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal" >Labjournal</a></li><li class="active"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials" >Materials</a><ul class="menu3"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Strains" >Strains</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Media" >Media</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Enzymes" >Enzymes</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Buffers" >Buffers</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Stock_Solution" >Stock Solution</a></li><li class="active"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Primers" >Primers</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Equipment" >Equipment</a></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/PCR_buffers_and_mastermixtures" >PCR buffers and mastermixtures</a></li></ul></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods" >Methods</a></li></ul></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Team" >Team</a></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Gallery" >Gallery</a></li></ul>
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<ul class="menu"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt" >Home</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Project" >Project</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results" >Results</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/PolicyandPractices" >Policy & Practices</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Achievements" >Achievements</a></li><li class="active"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook" >Notebook</a><ul class="menu2"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal" >Labjournal</a></li><li class="active"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials" >Materials</a><ul class="menu3"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Strains" >Strains</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Media" >Media</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Enzymes" >Enzymes</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Buffers" >Buffers</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Stock_Solution" >Stock Solution</a></li><li class="active"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Primers" >Primers</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Equipment" >Equipment</a></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/PCR_buffers_and_mastermixtures" >PCR buffers and mastermixtures</a></li></ul></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods" >Methods</a></li></ul></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Team" >Team</a></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Gallery" >Gallery</a></li></ul>
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Revision as of 16:56, 17 October 2014

Home


Pathway

RBS_CHI_pre
GTAGAGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTCCTCCAGTGTCCGTTAC
 
RBS_CHS_pre
GTAGAGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCGTCCTCCGTTGACATGAAGGCG
 
CHI_psb1c3_Pre
TAATAAGAATTCGCGGCCGCTTCTAGATGGCTCCTCCAGTGTCCGTTAC
 
CHI_psb1c3_Suf2
TTATTACTGCAGCGGCCGCTACTAGTACTAGACTCCAATCACTGGAATAGTAGATTTCTCGG
 
CHS_psb1c3_Pre2
TAATAAGAATTCGCGGCCGCTTCTAGATGGCGTCCTCCGTTGACATGAAGGCGATCAGAG
 
CHS_pscb1c3_Suf2
TTATTACTGCAGCGGCCGCTACTAGTATTAGACGGGAACCGTCACGGTGGTTGGGAC
 
QCIII_CHI_F
GAAGTTTCTGGATGTTTTCCGGAGTGAAACTTTTCCACCTGG
 
QCIII_CHI_R
CCAGGTGGAAAAGTTTCACTCCGGAAAACATCCAGAAACTTC
 
QCI_CHI_F
CGGCGATAGGTGTGTATCTGGAAGAGAGTGCTATTCCTTTTCTGGCCG
 
QCI_CHI_R
CGGCCAGAAAAGGAATAGCACTCTCTTCCAGATACACACCTATCGCCG
 
DFR_mut_EcoRI_577_F
TACGCAGCAGAAAACAATCTCGAATTTATCAGTATCATTCCACCGC
 
DFR_mut_EcoRI_577_R
ATGCGTCGTCTTTTGTTAGAGCTTAAATAGTCATAGTAAGGTGGCG
 
DFR_mut2_EcoRI_622_F
CGCCACCTTACTATGACTATTTAAGCTCTAACAAAAGACGACGCAT
 
DFR_mut2_EcoRI_622_R
GCGGTGGAATGATACTGATAAATTCGAGATTGTTTTCTGCTGCGTA
 
DFR_Prefix
GCGCGAATTCGCGGCCGCTTCTAGATGGTTTCTAGTACAATTAACGAGAC
 
DFR_Suffix
GCGCTACTAGTAGCGGCCGCTGCAGTTAAGCAGATCCATTTTCATGATGT
 
DFR_RBS_Prefix
GTAGGAATTCGCGGCCGCTTCTAGAAAGAGGAGAAATACTAGATGGTTTCTAGTACAATTAACGAGACAC
 
F3H_Prefix
GATGGAATTCGCGGCCGCTTCTAGATGGCTCCTTCAACTCTCAC
 
F3H_RBS_Prefix
GATGGAATTCGCGGCCGCTTCTAGAAAGAGGAGAAATACTAGATGGCTCCTTCAACTCTCAC
 
F3H_Suffix
GCGCCTGCAGCGGCCGCTACTAGTATCAAGCGAAAATTTCGTCTGCG
 
F3H_mut_EcoRI_fwd_820_F
GCCGACCACCAAGCAGTTGTGAACTCCAATTCTAGCAGAATGTCAAT
 
F3H_mut_EcoRI_fwd_820_R
ATTGACATTCTGCTAGAATTGGAGTTCACAACTGCTTGGTGGTCGGC
 
F3H_mut_PstI_fwd_1047_F
GAAGTGGAGAAGGCCAAGCTGCAAATGACACCCAAGAGC
 
F3H_mut_PstI_fwd_1047_R
GCTCTTGGGTGTCATTTGCAGCTTGGCCTTCTCCACTTC

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Scaffold

Scaff pET21a+ up
ATATCATATGGGATCTACCAAAGCAGATATTGGCAC
 
Scaff pET21a+ lo
ATATCTCGAGAGATCCGCTGCCGCTCCCACTA
 
Scaff-His up 2 right
ATGCTAGGAATTCGCGGCCGCTTCTAGATGGGATCTACCAAAGCAGATATTGGCACC
 
Scaff-His lo
TATACTGCAGCGGCCGCTACTAGTATTAGTGGTGGTGGTGGTGGTGCTCG
 
SHC up
ATATGAATTCGCGGCCGCTTCTAGATGGGATCTACCAAAGCAGATATTGGC
 
SHC lo
ATATCTGCAGCGGCCGCTACTAGTATCAGCAGTGGTGGTGGTGGTG
 
SH3-F
ATGCTAGGAATTCGCGGCCGCTTCTAGAAGATCTGCCGAATATGTTCGTGCACTGTTTGA
 
SH3-R
TCATCACTGCAGCGGCCGCTACTAGTAGGATCCTGAGCCACTACCACTACC
 
PDZ-F
ATGCTAGGAATTCGCGGCCGCTTCTAGAAGATCTCTGCAACGTCGTCGTGTTACCGTTCG
 
PDZ-R
TCATCACTGCAGCGGCCGCTACTAGTAGGATCCGCTGCCGCTCCCACTACCG
 
GBD-Met-F
ATGCTAGGAATTCGCGGCCGCTTCTAGATGAGATCTACCAAAGCAGATATTGGCACC
 
GBD-R
TCATCACTGCAGCGGCCGCTACTAGTAGGATCCGCTACCACTGCCGCTACCTGAACC
 
Prefix
GTTTCTTCGAATTCGCGGCCGCTTCTAG
 
Suffix
GTTTCTTCCTGCAGCGGCCGCTACTAGTA

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