Team:Aachen/Project/Gal3
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After we received the collection of pSBX-expression vectors from Team Heidelberg, we used Gibson assembly to make K1319020 from K1319003 and pSBX1A3, which is the translational unit for a mRFP-Gal3 fusion protein with a C-terminal 6xHis tag: | After we received the collection of pSBX-expression vectors from Team Heidelberg, we used Gibson assembly to make K1319020 from K1319003 and pSBX1A3, which is the translational unit for a mRFP-Gal3 fusion protein with a C-terminal 6xHis tag: | ||
- | <center>{{Team:Aachen/Figure|Aachen_K1319020.png|width= | + | <center>{{Team:Aachen/Figure|Aachen_K1319020.png|width=800px|title=K1319009|This BioBrick is a construction intermediate of K1319003 (gal3), E1010 (mRFP), K1319007 (6xHis tag) to K1319020 (translational unit of the fusion protein).}}</center> |
We also cloned our K1319003 into the pET17 expression vector and expressed all combinations of fusion proteins in E. coli BL21(DE3). A SDS-PAGE showed that all fusion proteins were fully translated: | We also cloned our K1319003 into the pET17 expression vector and expressed all combinations of fusion proteins in E. coli BL21(DE3). A SDS-PAGE showed that all fusion proteins were fully translated: |
Revision as of 23:08, 16 October 2014
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