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Team:HokkaidoU Japan/Notebook/Pre experiment/mRFP-expression-construct
From 2014.igem.org
(Difference between revisions)
| Line 205: | Line 205: | ||
<li class="step"> | <li class="step"> | ||
<div class="step-tile"> | <div class="step-tile"> | ||
| - | <h2 class="step-title">Gel Extraction | + | <h2 class="step-title">Gel Extraction</h2> |
| + | </div> | ||
| + | <div class="step-detail"> | ||
| + | <div class="step-detail-body"> | ||
| + | <ul class="step-detail-trials"> | ||
| + | <li> | ||
| + | <time>Sat Aug 16</time> | ||
| + | </li> | ||
| + | </ul> | ||
| + | <div class="step-recipe"> R0040-B0034-E1010-B0015 | ||
| + | pSB6A1</div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </li> | ||
| + | <li class="step"> | ||
| + | <div class="step-tile"> | ||
| + | <h2 class="step-title">Ethanol precipetation</h2> | ||
</div> | </div> | ||
<div class="step-detail"> | <div class="step-detail"> | ||
Revision as of 13:09, 17 October 2014
hogehoge
mRFP Expression Plasmid Construction
-
Start
-
Get genes
pSB6A1 -
Transformation
pSB6A1 1µL DNA to JM109 -
Liquid Culture
pSB6A1 -
Mini-prep
pSB6A1 -
PCR & PCR purification
R0040-B0034-E1010-B0015 from our stock 100up 200dn -
Digestion
Cut R0040-B0034-E1010-B0015 with EcoRI, PstI (using 10×H buffer) Cut pSB6A1 with EcoRI, PstI (using 10×H buffer) -
Gel Extraction
R0040-B0034-E1010-B0015 pSB6A1 -
Ethanol precipetation
R0040-B0034-E1010-B0015 pSB6A1 -
Dephosphorylation
R0040-B0034-E1010-B0015 -
Ligation
Ligate R0040-B0034-E1010-B0015 with pSB6A1 -
Transformation
R0040-B0034-E1010-B0015 on pSB6A1 5µL DNA to DH5α Turbo -
Liquid Culture
R0040-B0034-E1010-B0015 on pSB6A1 -
Mini-prep
R0040-B0034-E1010-B0015 on pSB6A1 -
Complete!
