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Team:Tokyo-NoKoGen
From 2014.igem.org
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<h2>Overview</h2> | <h2>Overview</h2> | ||
| - | <p>Many pesticides use neurotoxins that show toxicity not only to insects but also towards other species, including mammals. We therefore set out to construct an E. coli that can safely eradicate cockroaches but not affect mammals.</p> | + | <!--<p>Many pesticides use neurotoxins that show toxicity not only to insects but also towards other species, including mammals. We therefore set out to construct an E. coli that can safely eradicate cockroaches but not affect mammals.</p> |
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| + | <p>Cockroaches and many other insects store their energy in the form of trehalose and use the enzyme trehalase to convert it to glucose when needed. Our engineered E. coli will overexpress the enzymes OtsA and OtsB to produce trehalose, and glucose-3-dehydrogenase to convert trehalose to 3,3’-diketotrehalose, an inhibitor of trehalase that is not toxic for mammals. Our engineered Exterminator coli will help eradicate roaches from homes without harming humans or pets.</p>--> | ||
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| + | <p>Pesticide to harmful insects, such as roaches, puts human and environment at risk, so more safety pesticide is needed. Our project for iGEM 2014 is “Exterminator coli”. We engineered <i>E.coli</i> that produces an inhibitor of metabolic pathway for insects and exterminates insects such as roaches. In our project, we can produce pesticide that has no effect for mammals and environment by just one kind of <i>E.coli</i>. </p><br> | ||
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| + | <p>Roaches and many other insects store their energy in the form of trehalose and use the enzyme, “trehalase” which converts trehalose to glucose when needed. While, mammals store their energy in the form of glycogen, so trehalase is not used for conversion it to glucose. Therefore trehalase inhibitor is used as pesticide which isn’t harmful to mammals but roaches. </p><br> | ||
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| + | <p>We engineered E.coli that produces trehalase inhibitor, 3, 3´-diketotorehalose (3, 3´-dkT), as harmless pesticide to environment and mammals. Our engineered <i>E. coli </i>will overexpress <i>otsA</i> and <i>otsB</i> which produce enzymes convert glucose-6-phosphate and UDP-glucose to trehalose, and glucose-3-dehydrogenase (G3DH) which converts trehalose to 3, 3’-dkT. Therefore our <i>E.coli</i> can continue to produce 3, 3’-dkT by using glucose in insects. Just what we do is that make roaches eat <i>E.coli</i> we engineered.</p> | ||
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<img src="https://static.igem.org/mediawiki/2014/4/4a/NoKo14_Ecoli.jpg"><br> | <img src="https://static.igem.org/mediawiki/2014/4/4a/NoKo14_Ecoli.jpg"><br> | ||
<img src="https://static.igem.org/mediawiki/2014/5/53/Noko14_Glucose.png"> | <img src="https://static.igem.org/mediawiki/2014/5/53/Noko14_Glucose.png"> | ||
Revision as of 09:53, 16 October 2014
Overview
Pesticide to harmful insects, such as roaches, puts human and environment at risk, so more safety pesticide is needed. Our project for iGEM 2014 is “Exterminator coli”. We engineered E.coli that produces an inhibitor of metabolic pathway for insects and exterminates insects such as roaches. In our project, we can produce pesticide that has no effect for mammals and environment by just one kind of E.coli.
Roaches and many other insects store their energy in the form of trehalose and use the enzyme, “trehalase” which converts trehalose to glucose when needed. While, mammals store their energy in the form of glycogen, so trehalase is not used for conversion it to glucose. Therefore trehalase inhibitor is used as pesticide which isn’t harmful to mammals but roaches.
We engineered E.coli that produces trehalase inhibitor, 3, 3´-diketotorehalose (3, 3´-dkT), as harmless pesticide to environment and mammals. Our engineered E. coli will overexpress otsA and otsB which produce enzymes convert glucose-6-phosphate and UDP-glucose to trehalose, and glucose-3-dehydrogenase (G3DH) which converts trehalose to 3, 3’-dkT. Therefore our E.coli can continue to produce 3, 3’-dkT by using glucose in insects. Just what we do is that make roaches eat E.coli we engineered.
Our E.coli products 3'3-diketotrehlose (red triangle) from glucose(yellow circle).
3'3-diketotrehalose inhibit the action of trehalase, which is one of carbon source in insects.
