Team:Tokyo-NoKoGen/Safety

	Project	Team	Result	Parts	Protocols	Notebook	Safety	Human Practice	Achievement

What is the risk of our project?

　　In our project, we expect the cockroaches to eat the trehalase inhibitor, 3,3’-dkT, synthesized by our engineered E. coli on a solid medium. However, one possible problem is that E. coli on the surface of the roach’s body or legs may be carried outside of the trap and diffused. The E. coli may potentially spread in the environment and affect the ecosystem by acting on other insects.

　　To help prevent the unwanted spread of our engineered E. coli, we expect the future addition of a light-inducible lysis device. This would allow the E coli to grow well and produce the insecticide while in the trap in the dark. However, any E. coli that exits the dark roach habitat would die once exposed to light. A possible arrangement may involve placing a T4 phage lysis cassette (T4 endolysin and T4 holin) under the control of a light-inducible promoter system (e.g. green-light inducible CcaS/CcaR two-component system).

Our training

We have already had safety training.

All members are given a detailed training about basic lab safety, such as how to safely handle reagents and equipment (autoclave, burners, etc.). Every week, we have a short meeting to discuss any safety issues that come up.We also learn how to handle genetically modified organisms during experiments and how to treat before disposing them.

>Laboratory safety training requirements of our institution (Japanese only)

Our local rules and regulations

Dr. Akinori Koukitu is responsible for biological safety at our institution.

We submitted the details of our projects to him. We follow his advice.

>Biosafety guidelines of our institution (Japanese only)

>The regulations that govern biosafety in research laboratories.(Japanese only)

Risks of our project

The safety and health of team members or other people working in the lab will not be affected by our project.

The only species we use is Escherichia coli, all of which are classified as risk group1 and they do not harm human health.

To minimize risks to team members, we have a strict policy that anyone who enters the lab must wear goggles and lab coat, and anyone doing experiments must also wear gloves. Eating and drinking is strictly forbidding in our lab.

To minimize risks to public health and environment, we ensure that all biological materials are promptly autoclaved after use and disposed of appropriately. Furthermore, we follow our University and country regulations and guidelines for collecting and disposing of any toxic waste generated; our facility is already equipped to handle such wastes.

Biological materials and reagents used in our experiments are never brought outside the laboratory.

To minimize the risks of malicious misuse, the lab facilities are only unlocked when experiments are carried out, so our Biobrick parts and reagents cannot be stolen.