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Team:Macquarie Australia/WetLab/Protocols/Nanodrop
From 2014.igem.org
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<h3>NanoDrop</h3> | <h3>NanoDrop</h3> | ||
| - | + | <h4>NANODROP for THERMO SCIENTIFIC NanoDrop 2000 Spectrophotometer</h4> | |
| - | <h4 | + | |
| - | + | <ul style="list-style-type: decimal;"> | |
| - | <li> | + | <li>Pipette 1-2µl of blanking buffer onto the bottom pedestal, lower the arm and click the BLANK button. The blank solution is the same buffer that the DNA of interest is dissolved in.</li> |
| - | Pipette 1-2µl of blanking buffer onto the bottom pedestal, lower the arm and click the BLANK button. The blank solution is the same buffer that the DNA of interest is dissolved in. | + | <li>Wipe away the blank. Pipette 1-2µl of DNA sample and hit MEASURE</li> |
| - | </li> | + | <li>Wipe away the last sample to prepare for the next sample, and repeat.</li> |
| - | <li> | + | </ul> |
| - | Wipe away the blank. Pipette 1-2µl of DNA sample and hit MEASURE | + | |
| - | </li> | + | |
| - | <li> | + | |
| - | Wipe away the last sample to prepare for the next sample, and repeat. | + | |
| - | </li> | + | |
| - | |||
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</div> | </div> | ||
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</section> | </section> | ||
</body> | </body> | ||
</html> | </html> | ||
Revision as of 05:22, 16 October 2014
NanoDrop
NANODROP for THERMO SCIENTIFIC NanoDrop 2000 Spectrophotometer
- Pipette 1-2µl of blanking buffer onto the bottom pedestal, lower the arm and click the BLANK button. The blank solution is the same buffer that the DNA of interest is dissolved in.
- Wipe away the blank. Pipette 1-2µl of DNA sample and hit MEASURE
- Wipe away the last sample to prepare for the next sample, and repeat.
