Team:TU Darmstadt/Materials/Primers
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Revision as of 16:34, 16 October 2014
Pathway
RBS_CHI_pre |
GTAGAGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTCCTCCAGTGTCCGTTAC |
RBS_CHS_pre |
GTAGAGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCGTCCTCCGTTGACATGAAGGCG |
CHI_psb1c3_Pre |
TAATAAGAATTCGCGGCCGCTTCTAGATGGCTCCTCCAGTGTCCGTTAC |
CHI_psb1c3_Suf2 |
TTATTACTGCAGCGGCCGCTACTAGTACTAGACTCCAATCACTGGAATAGTAGATTTCTCGG |
CHS_psb1c3_Pre2 |
TAATAAGAATTCGCGGCCGCTTCTAGATGGCGTCCTCCGTTGACATGAAGGCGATCAGAG |
CHS_pscb1c3_Suf2 |
TTATTACTGCAGCGGCCGCTACTAGTATTAGACGGGAACCGTCACGGTGGTTGGGAC |
QCIII_CHI_F |
GAAGTTTCTGGATGTTTTCCGGAGTGAAACTTTTCCACCTGG |
QCIII_CHI_R |
CCAGGTGGAAAAGTTTCACTCCGGAAAACATCCAGAAACTTC |
QCI_CHI_F |
CGGCGATAGGTGTGTATCTGGAAGAGAGTGCTATTCCTTTTCTGGCCG |
QCI_CHI_R |
CGGCCAGAAAAGGAATAGCACTCTCTTCCAGATACACACCTATCGCCG |
DFR_mut_EcoRI_577_F |
TACGCAGCAGAAAACAATCTCGAATTTATCAGTATCATTCCACCGC |
DFR_mut_EcoRI_577_R |
ATGCGTCGTCTTTTGTTAGAGCTTAAATAGTCATAGTAAGGTGGCG |
DFR_mut2_EcoRI_622_F |
CGCCACCTTACTATGACTATTTAAGCTCTAACAAAAGACGACGCAT |
DFR_mut2_EcoRI_622_R |
GCGGTGGAATGATACTGATAAATTCGAGATTGTTTTCTGCTGCGTA |
DFR_Prefix |
GCGCGAATTCGCGGCCGCTTCTAGATGGTTTCTAGTACAATTAACGAGAC |
DFR_Suffix |
GCGCTACTAGTAGCGGCCGCTGCAGTTAAGCAGATCCATTTTCATGATGT |
DFR_RBS_Prefix |
GTAGGAATTCGCGGCCGCTTCTAGAAAGAGGAGAAATACTAGATGGTTTCTAGTACAATTAACGAGACAC |
F3H_Prefix |
GATGGAATTCGCGGCCGCTTCTAGATGGCTCCTTCAACTCTCAC |
F3H_RBS_Prefix |
GATGGAATTCGCGGCCGCTTCTAGAAAGAGGAGAAATACTAGATGGCTCCTTCAACTCTCAC |
F3H_Suffix |
GCGCCTGCAGCGGCCGCTACTAGTATCAAGCGAAAATTTCGTCTGCG |
F3H_mut_EcoRI_fwd_820_F |
GCCGACCACCAAGCAGTTGTGAACTCCAATTCTAGCAGAATGTCAAT |
F3H_mut_EcoRI_fwd_820_R |
ATTGACATTCTGCTAGAATTGGAGTTCACAACTGCTTGGTGGTCGGC |
F3H_mut_PstI_fwd_1047_F |
GAAGTGGAGAAGGCCAAGCTGCAAATGACACCCAAGAGC |
F3H_mut_PstI_fwd_1047_R |
GCTCTTGGGTGTCATTTGCAGCTTGGCCTTCTCCACTTC |
Scaffold
Scaff pET21a+ up |
ATATCATATGGGATCTACCAAAGCAGATATTGGCAC |
Scaff pET21a+ lo |
ATATCTCGAGAGATCCGCTGCCGCTCCCACTA |
Scaff-His up 2 right |
ATGCTAGGAATTCGCGGCCGCTTCTAGATGGGATCTACCAAAGCAGATATTGGCACC |
Scaff-His lo |
TATACTGCAGCGGCCGCTACTAGTATTAGTGGTGGTGGTGGTGGTGCTCG |
SHC up |
ATATGAATTCGCGGCCGCTTCTAGATGGGATCTACCAAAGCAGATATTGGC |
SHC lo |
ATATCTGCAGCGGCCGCTACTAGTATCAGCAGTGGTGGTGGTGGTG |
SH3-F |
ATGCTAGGAATTCGCGGCCGCTTCTAGAAGATCTGCCGAATATGTTCGTGCACTGTTTGA |
SH3-R |
TCATCACTGCAGCGGCCGCTACTAGTAGGATCCTGAGCCACTACCACTACC |
PDZ-F |
ATGCTAGGAATTCGCGGCCGCTTCTAGAAGATCTCTGCAACGTCGTCGTGTTACCGTTCG |
PDZ-R |
TCATCACTGCAGCGGCCGCTACTAGTAGGATCCGCTGCCGCTCCCACTACCG |
GBD-Met-F |
ATGCTAGGAATTCGCGGCCGCTTCTAGATGAGATCTACCAAAGCAGATATTGGCACC |
GBD-R |
TCATCACTGCAGCGGCCGCTACTAGTAGGATCCGCTACCACTGCCGCTACCTGAACC |
Prefix |
GTTTCTTCGAATTCGCGGCCGCTTCTAG |
Suffix |
GTTTCTTCCTGCAGCGGCCGCTACTAGTA |