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Team:TU Darmstadt/Materials/Buffers
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Revision as of 00:38, 16 October 2014
Buffers
Tris (1M, pH 7,5)
- 60,5g Tris base
- adjust pH to 7,5 using 5M HCl
- add ddH2O up to 500 mL of volume
1x PBS
- 8.18 g NaCl (140 mM)
- 0.2 g KCl (2.7 mM)
- 1.77 g Na2HPO4 (10 mM)
- 0.24 g KH2PO4 (1.8 mM)
- add ddH2O up to 1 L of volume
- adjust to pH 7.4
TE buffer
- 10 mM Tris, bring to pH 8.0 with HCl
- 1 mM EDTA (Ethylenediaminetetraacetic acid)
50x TAE
- 242 g Tris base in water
- add 57.1 mL glacial acetic acid
- 100 mL of 500 mM EDTA (pH 8.0) solution
- add ddH2O up to 1 L of volume
