Team:Bielefeld-CeBiTec/Project/Isobutanol/GeneticalApproach

From 2014.igem.org

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   <h6>Genetical Approach</h6>
   <h6>Genetical Approach</h6>
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     <p>There were different genetical approaches to produce isobutanol with various organisms like cyanobacteria, <i>Bacillus subtilis</i>, <i>Escherichia coli</i> and some more. We aim to reproduce the production pipeline which was done by iGEM Team Formosa 2011 and 2012.
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     <p>There are different genetical approaches to produce isobutanol with various organisms like cyanobacteria, <i>B. subtilis </i>, <i>E. coli</i> and some more. We aim to reproduce the production pipeline which was done by iGEM Team Formosa 2011 and 2012 in <i>E. coli</i>. The iGEM Team Formosa identified the four proteins alsS, ilvC, ilvD and kivD as required for the production of isobutanol in <i>E. coli</i>. We want to reproduce their idea without the temperature shift and with all genes on one plasmid. Additionally we want to combine this plasmid with the adhA from <i>L. lactis</i>.</p>
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By combining the required enzymes we will first create a production BioBrick with four enzymes. A possible enhancement could be achieved by the alcohol dehydrogenase (<i>adhA</i>).</p>
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         <img src="https://static.igem.org/mediawiki/2014/6/67/Bielefeld_CeBiTec_2014-09-01_Isobutanol_weiss_Merck_3.png" width="350px" align="center">
         <img src="https://static.igem.org/mediawiki/2014/6/67/Bielefeld_CeBiTec_2014-09-01_Isobutanol_weiss_Merck_3.png" width="350px" align="center">
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  <h6>AlsS (α-acetolactate synthase)</h6>
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  <h6>IlvC (Ketol-acid reductoisomerase)</h6>
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  <h6>IlvD (Dihydroxyacid dehydratase)</h6>
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  <h6>KivD (α-ketoisovalerate decarboxylase)</h6>
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  <h6>AdhA (Alcoholdehydrogenase)</h6>
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Revision as of 19:45, 15 October 2014


Isobutanol

Genetical Approach

There are different genetical approaches to produce isobutanol with various organisms like cyanobacteria, B. subtilis , E. coli and some more. We aim to reproduce the production pipeline which was done by iGEM Team Formosa 2011 and 2012 in E. coli. The iGEM Team Formosa identified the four proteins alsS, ilvC, ilvD and kivD as required for the production of isobutanol in E. coli. We want to reproduce their idea without the temperature shift and with all genes on one plasmid. Additionally we want to combine this plasmid with the adhA from L. lactis.

AlsS (α-acetolactate synthase)
IlvC (Ketol-acid reductoisomerase)
IlvD (Dihydroxyacid dehydratase)
KivD (α-ketoisovalerate decarboxylase)
AdhA (Alcoholdehydrogenase)

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