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Team:TU Darmstadt/Notebook/Methods
From 2014.igem.org
(Difference between revisions)
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<p>For DNA | <p>For DNA | ||
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Agarose_gel_electrophoresis" title="Opens internal link in current window" class="internal-link">Agarose gel electrophoresis</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Dephosphorylation" title="Opens internal link in current window" class="internal-link">Dephosphorylation</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/DNA_ligation" title="Opens internal link in current window" class="internal-link">DNA ligation</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/DNA_quantification_with_NanoDrop" title="Opens internal link in current window" class="internal-link">DNA quantification with NanoDrop</a> |
</p> | </p> | ||
<p>- <a href="http://www.eurofinsgenomics.eu/media/892645/samplesubmissionguide_valuereadtube.pdf" title="Link to eurofinsgenomics.eu" target="_blank" class="external-link-new-window">DNA sequencing</a> | <p>- <a href="http://www.eurofinsgenomics.eu/media/892645/samplesubmissionguide_valuereadtube.pdf" title="Link to eurofinsgenomics.eu" target="_blank" class="external-link-new-window">DNA sequencing</a> | ||
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/PCR_purification" title="Opens internal link in current window" class="internal-link">PCR purification</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/PCR_with_Pfu_polymerase" title="Opens internal link in current window" class="internal-link">PCR with Pfu polymerase</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Plasmid_preparation" title="Opens internal link in current window" class="internal-link">Plasmid preparation</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Restriction_digest" title="Opens internal link in current window" class="internal-link">Restriction digest</a> |
</p> | </p> | ||
<p>For Proteins | <p>For Proteins | ||
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/SDS-PAGE" title="Opens internal link in current window" class="internal-link">SDS-PAGE</a> |
</p> | </p> | ||
<p> </p> | <p> </p> | ||
<p><b>In vivo methods</b> | <p><b>In vivo methods</b> | ||
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Bacterial_cell_culture" title="Opens internal link in current window" class="internal-link">Bacterial cell culture</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Cell_counting_plating" title="Opens internal link in current window" class="internal-link">Cell counting/plating</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Chemically_competent_cells" title="Opens internal link in current window" class="internal-link">Chemically competent cells</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Colony_PCR_with_Taq_polymerase" title="Opens internal link in current window" class="internal-link">Colony PCR with Taq polymerase</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Glycerine_stocks" title="Opens internal link in current window" class="internal-link">Glycerine stocks</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Heat_shock_transformation" title="Opens internal link in current window" class="internal-link">Heat shock transformation</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Protein_expression" title="Opens internal link in current window" class="internal-link">Protein expression</a></p></header><ul style="line-height: 1.5em; list-style: none; margin: 0.3em 50px 0px; padding: 0px; text-align: justify; "></ul><p style="margin: 0px 50px 15px; line-height: 1.5em; text-align: justify; color: white; font-family: 'Arial regular'; font-size: medium; "> </p></div><div id="c245" class="csc-default"><p><b>Anthocyanin extraction methods</b> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/37%_hydrochloric_acid" title="Opens internal link in current window" class="internal-link">37% hydrochloric acid</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Dichloromethane" title="Opens internal link in current window" class="internal-link">Dichloromethane</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Methanol" title="Opens internal link in current window" class="internal-link">Methanol</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Ethanol" title="Opens internal link in current window" class="internal-link">Ethanol</a> |
</p> | </p> | ||
<p> </p> | <p> </p> | ||
Revision as of 19:20, 15 October 2014
Methods
This page outlines our standardized protocols we used in the iGEM project 2014. If we changed one of the protocols for a specific reason, we mentioned it in the laboratory notebook.
In vitro methods
For DNA
- DNA quantification with NanoDrop
For Proteins
- SDS-PAGE
In vivo methods
