Team:Hong Kong HKUST/wetlab/protocols

From 2014.igem.org

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<h4> <b>BD FACSAriaIII User Guide<b></h4>
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<h4> Dephosphorylation </h4>
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<h4> <b>Dephosphorylation</b> </h4>
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<a href="https://static.igem.org/mediawiki/2014/2/25/19._Ethanol_Precipitation.pdf">
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<h4> Ethanol Precipitation </h4>
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<h4> <b>Ethanol Precipitation</b> </h4>
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<a href="https://static.igem.org/mediawiki/2014/6/64/Gibson_assembly_master_mix-_assembly.pdf">
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<h4> Gibson Assembly </h4>
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<h4> <b>Gibson Assembly</b> </h4>
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<h4> Ligation </h4>
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<h4> <b>Ligation</b> </h4>
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<h4> Liquid Mediums and Agarose Plates </h4>
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<h4> <b>Liquid Mediums and Agarose Plates</b> </h4>
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<a href="https://static.igem.org/mediawiki/2014/8/8b/M9_Minimal_medium_protocol.pdf">
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<h4> M9 Minimal Medium </h4>
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<h4> <b>M9 Minimal Medium</b> </h4>
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<a href="https://static.igem.org/mediawiki/2014/2/2a/Mutagenesis_protocol_1_%28multiple_sites%29.pdf">
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<h4> Mutagenesis 1 (Multiple Sites)</h4>
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<h4> <b>Mutagenesis 1 <br>(Multiple Sites)</b></h4>
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<a href="https://static.igem.org/mediawiki/2014/1/1f/Mutagenesis_protocol_2_%28single_site%29.pdf">
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<h4> Mutagenesis 2 (Single Sites) </h4>
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<h4> <b>Mutagenesis 2 <br>(Single Sites)</b> </h4>
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<h4> Mutagenesis 3 (Single Sites) </h4>
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<h4> <b>Mutagenesis 3 <br>(Single Sites)</b> </h4>
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<h4> Oligonucleotide Phosphorylation and Annealing </h4>
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<h4> <b>Oligonucleotide Phosphorylation and Annealing</b> </h4>
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<h4> PCR (routine <i>Taq</i>)</h4>
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<h4> <b>PCR (routine <i>Taq</i>)</b></h4>
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<h4> PCR (Phusion) </h4>
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<h4> <b>PCR (Phusion)</b> </h4>
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<a href="https://static.igem.org/mediawiki/2014/c/c5/Protocol_for_Vent_PCR.pdf">
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<h4> <b>PCR (Vent)</b> </h4>
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<a href="https://static.igem.org/mediawiki/2014/7/76/RPU_Measurement_Method_.pdf">
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<h4> RPU Measurement </h4>
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<h4> <b>RPU Measurement</b> </h4>
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<a href="https://static.igem.org/mediawiki/2014/4/4d/The_Inoue_Method_for_Preparation_and_Transformation_of_Competent_E.pdf">
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<h5 style="font-size:20px"> The Inoue Method for Preparation and Transformation of Competent E. Coli: "Ultra-Competent" Cells</h5>
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<h4> <b>The Inoue Method for Preparation and Transformation of Competent E. Coli: "Ultra-Competent" Cells</b></h4>
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Revision as of 17:08, 15 October 2014



Protocols

We have a variety of protocols that we followed for working on our project. The step-by-step procedures of each technique can be found here!

Home

Pneumosensor

Riboregulator

Human Practice

Team

WetLab

Achievement

Retrieved from "http://2014.igem.org/Team:Hong_Kong_HKUST/wetlab/protocols"