Team:Caltech/week3.html

From 2014.igem.org

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<b><a href='Overview.html'>Overview</a></b><br><br>
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<b><a href='Notebook'>Overview</a></b><br><br>
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<b><a href='week1.html'>Week 1</a></b><br><br>
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<b><a href='week1'>Week 1</a></b><br><br>
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<b><a href='week2.html'>Week 2</a></b><br><br>
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<b><a href='week2'>Week 2</a></b><br><br>
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<b><a href='week3.html'>Week 3</a></b><br><br>
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<b><a href='week3'>Week 3</a></b><br><br>
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<b><a href='week4.html'>Week 4</a></b><br><br>
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<b><a href='week4'>Week 4</a></b><br><br>
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<b><a href='week5.html'>Week 5</a></b><br><br>
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<b><a href='week5'>Week 5</a></b><br><br>
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<b><a href='week6.html'>Week 6</a></b><br><br>
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<b><a href='week6'>Week 6</a></b><br><br>
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<b><a href='week7.html'>Week 7</a></b><br><br>
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<b><a href='week7'>Week 7</a></b><br><br>
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<b><a href='week8.html'>Week 8</a></b><br><br>
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<b><a href='week8'>Week 8</a></b><br><br>
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<b><a href='week9.html'>Week 9</a></b><br><br>
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<b><a href='week9'>Week 9</a></b><br><br>
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<b><a href='week10.html'>Week 10</a></b><br><br>
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<b><a href='week10'>Week 10</a></b><br><br>
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<b><a href='week11.html'>Week 11</a></b><br><br>
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<b><a href='week11'>Week 11</a></b><br><br>
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<b><a href='week12.html'>Week 12</a></b><br><br>
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<b><a href='week12'>Week 12</a></b><br><br>
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<tr><td colspan=3><h1>Week Two</h1></td></tr>
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<tr><td colspan=3><h1>Week Three</h1></td></tr>
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<ul><li>Discussion of fallout from Friday's meeting, paper reading</li>
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<ul><li>Discussion of fallout from Friday's meeting</li>
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     <li>Colony PCR and Minipreps of liquid cultures of colonies picked over the weekend</li>
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     <li>More paper-reading to determine what's wrong with our new quorum-sensing system candidates</li>
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     <li>TXTL workshop starts today</li>
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     <li>Designed geneblocks for our new systems and subgroup projects</li>
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<ul><li>Discussed alternatives to current ComQXPA system, including 2-cell system (not all in 1 cell)</li>
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<ul><li>Lab cleanup. Reorganized -20 freezer samples.</li>
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     <li>Gibson assembly of another combinatorial promoter using pKS001 backbone, lacI geneblock, ____ promoter geneblock, and sfGFP geneblock.</li>
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     <li>World Cup watching. Tim Howard's a boss</li>
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     <li>Transformation of Gibson constructs into JM109 cells</li>
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     <li>Work on the team wiki while we wait for our next batch of geneblocks to ship</li>
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    <li>Linear combinatorial constructs created via PCR of pAA001 (minipreps) to be tested in TXTL</li>
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<ul><li>Linear constructs and plasmid TXTL reactions of A90 promoter (pAA001) were set up and run</li>
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<ul><li></li>
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     <li>Gibson assembly of plasmid pAA002 containing B83 promoter</li>
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     <li></li>
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     <li>PCR of Gibson assemblies of pAA002 to create linear constructs to test in TXTL</li>
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     <li></li>
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     <li>TXTL reactions of B83 promoter (linear frag. from pAA002) were set up and run</li>
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     <li></li>
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     <li>Transformation of pAA002 Gibson assemblies into JM109</li>
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<ul><li>Analysis of yesterday's TXTL reactions (run overnight)</li>
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<ul><li></li>
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     <li>Colony PCR and miniprep of bacterial colonies transformed with pAA002. Minipreps shipped for sequencing.</li>
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     <li></li>
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Latest revision as of 17:29, 3 July 2014


WELCOME TO iGEM 2014!

Your team has been approved and you are ready to start the iGEM season!
On this page you can document your project, introduce your team members, document your progress
and share your iGEM experience with the rest of the world!


Click here to edit this page!

Home Team Official Team Profile Project Parts Modeling Notebook Safety Attributions

Notebook

Apparently there's a calendar feature to take care of this too???


Overview

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week Three

Monday, 6/30/14
  • Discussion of fallout from Friday's meeting
  • More paper-reading to determine what's wrong with our new quorum-sensing system candidates
  • Designed geneblocks for our new systems and subgroup projects

Tuesday, 7/1/14
  • Lab cleanup. Reorganized -20 freezer samples.
  • World Cup watching. Tim Howard's a boss
  • Work on the team wiki while we wait for our next batch of geneblocks to ship

Wednesday, 7/2/14

Thursday, 7/3/14

Friday, 7/4/14

 It's the 4th of July!!!
'Murica...or something like that, I guess...


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