Team:Caltech/Notebook

From 2014.igem.org

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<b><a href='Overview.html'>Overview</a></b><br><br>
<b><a href='Overview.html'>Overview</a></b><br><br>
<b><a href='week1.html'>Week 1</a></b><br><br>
<b><a href='week1.html'>Week 1</a></b><br><br>
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<tr><td colspan=3><h1>Week One</h1></td></tr>
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<tr><td colspan=3><h1>Week Three</h1></td></tr>
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<h4>Tuesday, 6/17/14</h4>
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<h4>Monday, 6/30/14</h4>
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<ul><li>Get acquainted with summer logistics and lab basics</li>
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<ul><li>Discussion of fallout from Friday's meeting</li>
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     <li>Clean up lab space, stock lab supplies</li>
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    <li>More paper-reading to determine what's wrong with our new quorum-sensing system candidates</li>
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     <li>Designed geneblocks for our new systems and subgroup projects</li>
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<h4>Wednesday, 6/18/14</h4>
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<h4>Tuesday, 7/1/14</h4>
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<ul><li>Continue to discuss project and clean up lab space</li>
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<ul><li>Lab cleanup. Reorganized -20 freezer samples.</li>
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     <li>Designed PCR primers for combinatorial promoters subproject</li>
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     <li>World Cup watching. Tim Howard's a boss</li>
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    <li>Work on the team wiki while we wait for our next batch of geneblocks to ship</li>
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<h4>Thursday, 6/19/14</h4>
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<h4>Wednesday, 7/2/14</h4>
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<ul><li>Formulated plasmid design (pAA001) for the response regulation subproject</li>
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<ul><li></li>
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     <li>PCR run on plasmid backbone and sfGFP to create constructs with the proper overhangs for Gibson assembly</li>
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     <li>Gel electrophoresis for confirmation of PCR products' length</li>
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<ul><li>With the exception of the lacI gene, PCR products were confirmed via gel electrophoresis</li><ul>
 
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<h4>Friday, 6/20/14</h4>
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<h4>Thursday, 7/3/14</h4>
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<ul><li>Purification of PCR products created yesterday</li>
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     <li>Gibson assembly of purified products</li>
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    <li>Transformation of Gibson-assembled plasmids into JM109 E. coli</li>
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    <li>Meeting with Prof. Murray to discuss give an update on the project</li>
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<ul><li>Colonies were found and picked after transformation and incubation.</li></ul>
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<h4>Friday, 7/4/14</h4>
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<font size=+1>It's the 4th of July!!!
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'Murica...or something like that, I guess...</font>
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Revision as of 23:11, 1 July 2014


WELCOME TO iGEM 2014!

Your team has been approved and you are ready to start the iGEM season!
On this page you can document your project, introduce your team members, document your progress
and share your iGEM experience with the rest of the world!


Click here to edit this page!

Home Team Official Team Profile Project Parts Modeling Notebook Safety Attributions

Notebook

Apparently there's a calendar feature to take care of this too???

Overview

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week Three

Monday, 6/30/14
  • Discussion of fallout from Friday's meeting
  • More paper-reading to determine what's wrong with our new quorum-sensing system candidates
  • Designed geneblocks for our new systems and subgroup projects

Tuesday, 7/1/14
  • Lab cleanup. Reorganized -20 freezer samples.
  • World Cup watching. Tim Howard's a boss
  • Work on the team wiki while we wait for our next batch of geneblocks to ship

Wednesday, 7/2/14

Thursday, 7/3/14

Friday, 7/4/14

 It's the 4th of July!!!
'Murica...or something like that, I guess...


Retrieved from "http://2014.igem.org/Team:Caltech/Notebook"