Team:UC Davis/Electrochemistry

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Revision as of 03:47, 15 October 2014

UC Davis iGEM 2014

Electrode Choice

System Optimization

Coupling Enzymes

The Electrochemistry team acquired, selected, and optimized an electrode setup for the detection of NADH at low concentrations in a complex solution. Additionally, the team demonstrated the ability of the electrode setup to detect enzyme generated NADH over time, and thereby functionally deconvolute aldehyde profiles within a sample.

Electrode Choice

Having settled on NAD⁺ dependent Aldehyde Dehydrogenases as our method of differentiating between aldehydes, we needed to develop an efficient electrode system to detect enzyme activity via NADH.
Our Electrode system needed to:

Sensitive: have a low limit of detection for NADH
Reactive: Detect NADH with high linear range
Selective: Be robust to any possible solution components
Affordable: Cost accessible to the average consumer
Be efficient: Use a low sample volume
Be compatible: Be compatible with our, as well as other potentiostats
Be portable

System Optimization

Here is some sample text

Coupling Enzymes

Here is some sample text

@@ Line 57: / Line 57: @@
 <ul>
 <li>
-Detect NADH at low LOD (Sensitive)
+Sensitive: have  a low limit of detection for NADH
 </li>
 <li>
-Detect NADH with high linear range (Reactive)
+Reactive: Detect NADH with high linear range
 </li>
 <li>
-Be robust to any possible solution components (Selective)
+Selective: Be robust to any possible solution components
 </li>
 <li>
-Be cheap (low cost)
+Affordable: Cost accessible to the average consumer
 </li>
 <li>
-Use a low sample volume (be efficient)
+Be efficient: Use a low sample volume
 </li>
 <li>
-Be compatible and interfacaeble with potentiostat
+Be compatible: Be compatible with our, as well as other potentiostats
 </li>
 <li>
-Be Portable
+Be portable
 </li>
 </ul>