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Team:UC Davis/Electrochemistry
From 2014.igem.org
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| - | Having settled on NAD<sup>+</sup> dependent Aldehyde Dehydrogenases as our method of differentiating between aldehydes, we needed to develop an efficient electrode system to detect enzyme activity via NADH. | + | Having settled on NAD<sup>+</sup> dependent Aldehyde Dehydrogenases as our method of differentiating between aldehydes, we needed to develop an efficient electrode system to detect enzyme activity via NADH.<br></br> |
Our Electrode system needed to: | Our Electrode system needed to: | ||
<ul> | <ul> | ||
Revision as of 03:38, 15 October 2014
Electrode Choice
Electrode Choice
System Optimization
System Optimization
Coupling Enzymes
Coupling Enzymes
The Electrochemistry team acquired, selected, and optimized an electrode setup for the detection of NADH at low concentrations in a complex solution. Additionally, the team demonstrated the ability of the electrode setup to detect enzyme generated NADH over time, and thereby functionally deconvolute aldehyde profiles within a sample.
Electrode Choice
Having settled on NAD+ dependent Aldehyde Dehydrogenases as our method of differentiating between aldehydes, we needed to develop an efficient electrode system to detect enzyme activity via NADH.
Our Electrode system needed to:
- Detect NADH at low LOD (Sensitive)
- Detect NADH with high linear range (Reactive)
- Be robust to any possible solution components (Selective)
- Be cheap (low cost)
- Use a low sample volume (be efficient)
- Be compatible and interfacaeble with potentiostat
- Be Portable
System Optimization
Here is some sample text
Coupling Enzymes
Here is some sample text
