Team:Berlin/Project

From 2014.igem.org

(Difference between revisions)
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     <a href="https://2014.igem.org/Team:Berlin/Team" class="main-menue-links"><li>Team</li></a>
     <a href="https://2014.igem.org/Team:Berlin/Team" class="main-menue-links"><li>Team</li></a>
     <a href="https://2014.igem.org/Team:Berlin/Safety" class="main-menue-links"><li>Safety</li></a>
     <a href="https://2014.igem.org/Team:Berlin/Safety" class="main-menue-links"><li>Safety</li></a>
 +
    <a href="http://igem.berlin/contact.php" class="main-menue-links"><li>Contact</li></a>         
     <a href="https://2014.igem.org/Team:Berlin/Workshop" class="main-menue-links"><li>Workshop</li></a>
     <a href="https://2014.igem.org/Team:Berlin/Workshop" class="main-menue-links"><li>Workshop</li></a>
     <a href="https://2014.igem.org/Team:Berlin/Blog"><li>Blog</li></a>
     <a href="https://2014.igem.org/Team:Berlin/Blog"><li>Blog</li></a>
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     <li><a href="https://2014.igem.org/Team:Berlin/Team">Team</a></li>
     <li><a href="https://2014.igem.org/Team:Berlin/Team">Team</a></li>
     <li><a href="https://2014.igem.org/Team:Berlin/Safety">Safety</a></li>
     <li><a href="https://2014.igem.org/Team:Berlin/Safety">Safety</a></li>
 +
    <li><a href="http://igem.berlin/contact.php">Contact</a></li>
     <li><a href="https://2014.igem.org/Team:Berlin/Workshop">Workshop</a></li>
     <li><a href="https://2014.igem.org/Team:Berlin/Workshop">Workshop</a></li>
     <li><a href="https://2014.igem.org/Team:Berlin/Blog">Blog</a></li>
     <li><a href="https://2014.igem.org/Team:Berlin/Blog">Blog</a></li>
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       <div class="start-text-container">
       <div class="start-text-container">
-
         <h2><img src="https://static.igem.org/mediawiki/2014/f/ff/Team_Berlin_igem_questionmark.png" alt="" class="teaser-icons hidden-xs" />Discover Magnetic e.Coli</h2>
+
         <h2><img src="https://static.igem.org/mediawiki/2014/f/ff/Team_Berlin_igem_questionmark.png" alt="" class="teaser-icons hidden-xs" />Explore our Project:</h2>
           <div class="teaser-text-sub">
           <div class="teaser-text-sub">
             <div class="col-xs-12">
             <div class="col-xs-12">
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       <p>     
       <p>     
         <a name="animation">&nbsp;</a>
         <a name="animation">&nbsp;</a>
-
<iframe src="//player.vimeo.com/video/108832894?byline=0&amp;portrait=0&amp;color=0ecd28" width="700" height="393" frameborder="0" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe>
+
              Animation, jo!
     </div>
     </div>
   </div>     
   </div>     
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       <p>     
       <p>     
         <a name="lab-summary">&nbsp;</a>
         <a name="lab-summary">&nbsp;</a>
-
               Lab Diary, jo!
+
               <div class="sub-content-project">
 +
                <h2 class="sub-content-project-headline">Week 1: 02.04.2014 – 06.04.2014</h2>
 +
                    Cultivation of E. coli Nissle 1917<br/>
 +
                    <br/>
 +
                    Genomic DNA extraction of E.coli Nissle strain<br/>
 +
                    <br/>
 +
                    Production of BfR, FTNA1 and FTNA2<br/>
 +
                    Restriction digest<br/>
 +
                    PCR Purification<br/>
 +
                    Ligation Assay<br/>
 +
                    Transformation into DH5α-Cells<br/>
 +
              </div>
 +
              <div class="sub-content-project">
 +
                <h2 class="sub-content-project-headline">Week 2: 07.04.2014 – 13.04.2014</h2>
 +
                    Colony PCR to check the results<br/>
 +
                    <br/>
 +
                    Bfr, FTNA 1, FTNA 2 primar designed for amplification<br/>
 +
                    <br/>
 +
                    Transformation of pQE_80L into DH5α<br/>
 +
                    <br/>
 +
                    Cultivation of BfR, FTNA 1, FTNA 2 in LB<br/>
 +
              </div>
 +
              <div class="sub-content-project">
 +
                <h2 class="sub-content-project-headline">Week 3: 14.04.2014 – 20.04.2014</h2>
 +
                    Miniprep of the cells  from Week 2 <br/>
 +
                    <br/>
 +
                    DNA concentration determination and sequencing<br/>
 +
                    <br/>
 +
                    Expression of BfR, FTNA 1, FTNA 2 and induction with IPTG<br/>
 +
                    <br/>
 +
                    Production of a Mutaflor-Supression culture and streaking<br/>
 +
              </div>
 +
              <div class="sub-content-project">
 +
                <h2 class="sub-content-project-headline">week two!</h2>
 +
                  Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.
 +
              </div>
               <div class="sub-content-project">
               <div class="sub-content-project">
                 <h2 class="sub-content-project-headline">week one!</h2>
                 <h2 class="sub-content-project-headline">week one!</h2>
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                   Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.
                   Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.
               </div>
               </div>
-
 
+
                <h2 class="sub-content-project-headline">week one!</h2>
 +
                  Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.
 +
              </div>
 +
              <div class="sub-content-project">
 +
                <h2 class="sub-content-project-headline">week two!</h2>
 +
                  Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.
 +
              </div>
 +
              <div class="sub-content-project">
 +
                <h2 class="sub-content-project-headline">week one!</h2>
 +
                  Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.
 +
              </div>
 +
              <div class="sub-content-project">
 +
                <h2 class="sub-content-project-headline">week two!</h2>
 +
                  Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.
 +
              </div>
 +
              <div class="sub-content-project">
 +
                <h2 class="sub-content-project-headline">week one!</h2>
 +
                  Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.
 +
              </div>
 +
              <div class="sub-content-project">
 +
                <h2 class="sub-content-project-headline">week two!</h2>
 +
                  Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.
 +
              </div>
 +
                <h2 class="sub-content-project-headline">week one!</h2>
 +
                  Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.
 +
              </div>
 +
              <div class="sub-content-project">
 +
                <h2 class="sub-content-project-headline">week two!</h2>
 +
                  Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.
 +
              </div>
 +
              <div class="sub-content-project">
 +
                <h2 class="sub-content-project-headline">week one!</h2>
 +
                  Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.
 +
              </div>
 +
              <div class="sub-content-project">
 +
                <h2 class="sub-content-project-headline">week two!</h2>
 +
                  Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.
 +
              </div>
 +
              <div class="sub-content-project">
 +
                <h2 class="sub-content-project-headline">week one!</h2>
 +
                  Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.
 +
              </div>
 +
              <div class="sub-content-project">
 +
                <h2 class="sub-content-project-headline">week two!</h2>
 +
                  Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.
 +
              </div>
 +
                <h2 class="sub-content-project-headline">week one!</h2>
 +
                  Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.
 +
              </div>
 +
              <div class="sub-content-project">
 +
                <h2 class="sub-content-project-headline">week two!</h2>
 +
                  Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.
 +
              </div>
 +
              <div class="sub-content-project">
 +
                <h2 class="sub-content-project-headline">week one!</h2>
 +
                  Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.
 +
              </div>
 +
              <div class="sub-content-project">
 +
                <h2 class="sub-content-project-headline">week two!</h2>
 +
                  Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.
 +
              </div>
 +
              <div class="sub-content-project">
 +
                <h2 class="sub-content-project-headline">week one!</h2>
 +
                  Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.
 +
              </div>
 +
              <div class="sub-content-project">
 +
                <h2 class="sub-content-project-headline">week two!</h2>
 +
                  Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.
 +
              </div>
 +
              <div class="sub-content-project">
 +
                <h2 class="sub-content-project-headline">week two!</h2>
 +
                  Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.
 +
              </div>             
     </div>
     </div>
   </div>     
   </div>     
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           <a href="https://2014.igem.org/Team:Berlin" class="active"><li>Home |</li></a>
           <a href="https://2014.igem.org/Team:Berlin" class="active"><li>Home |</li></a>
           <a href="https://2014.igem.org/Team:Berlin/Project"><li>Project |</li></a>
           <a href="https://2014.igem.org/Team:Berlin/Project"><li>Project |</li></a>
-
           <a href="https://2014.igem.org/Team:Berlin/Team"><li>Team |</li></a>           
+
           <a href="http://igem.berlin/team.php"><li>Team |</li></a>           
-
           <a href="https://2014.igem.org/Team:Berlin/Safety">Safety |</li></a>
+
           <a href="http://igem.berlin/safety.php">Safety |</li></a>
-
           <a href="https://2014.igem.org/Team:Berlin/Workshop"><li>Workshop |</li></a>
+
           <a href="http://igem.berlin/contact.php"><li>Contact |</li></a>
-
           <a href="https://2014.igem.org/Team:Berlin/Blog"><li>Blog</li></a>          
+
          <a href="http://igem.berlin/workshop2014_neu.php"><li>Workshop |</li></a>
 +
           <a href="http://igem.berlin/blog.php"><li>Blog</li></a>        
         </ul>
         </ul>
       </div>
       </div>
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$( "html" ).css( "margin-top","-10px" );
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Revision as of 21:24, 14 October 2014

1

What is it all about?

  As previous iGEM teams have shown, synthesizing fully functional magnetosomes in E. coli is highly difficult as more than 60 highly regulated genes are involved. As a more feasible alternative, we simply want to synthesize magnetic nanoparticles in E. coli in order to attract cells with strong magnetic fields.
Therefore we want to use different strategies including manipulation of the iron homeostasis of E. coli, expression of different metal binding proteins such as ferritins and metallothioneins as well as a high-throughput growth medium optimization.

Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.

2

Visualisation

  Animation, jo!

3

Detailes Description

  MORE DETAILS, jo!

4

Flow Chart

  Flowchart, jo!

5

The Results

  Results, jo!

6

Lab Summary

 

Week 1: 02.04.2014 – 06.04.2014

Cultivation of E. coli Nissle 1917

Genomic DNA extraction of E.coli Nissle strain

Production of BfR, FTNA1 and FTNA2
Restriction digest
PCR Purification
Ligation Assay
Transformation into DH5α-Cells

Week 2: 07.04.2014 – 13.04.2014

Colony PCR to check the results

Bfr, FTNA 1, FTNA 2 primar designed for amplification

Transformation of pQE_80L into DH5α

Cultivation of BfR, FTNA 1, FTNA 2 in LB

Week 3: 14.04.2014 – 20.04.2014

Miniprep of the cells from Week 2

DNA concentration determination and sequencing

Expression of BfR, FTNA 1, FTNA 2 and induction with IPTG

Production of a Mutaflor-Supression culture and streaking

week two!

Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.

week one!

Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.

week two!

Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.

week one!

Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.

week two!

Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.

week one!

Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.

week two!

Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.

week one!

Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.

week two!

Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.

week one!

Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.

week two!

Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.

week one!

Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.

week two!

Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.

week one!

Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.

week two!

Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.

week one!

Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.

week two!

Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.

week one!

Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.

week two!

Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.

week one!

Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.

week two!

Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.

week two!

Furthermore, we will work with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used by any research lab or iGEM team in the world in order to remote control the cellular movement.
7

Notebook

  Notebook, jo!

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10623 Berlin
Phone: +49 1778800244
Mail: mail@igem.berlin
©2014 iGem Berlin, Layout/Umsetzung: Valerian Blos, Marius Wessel

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