Team:SF Bay Area DIYbio/Notebook

From 2014.igem.org

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Notebook
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<p><b>General Protocols</b></p>
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General Protocols</p>
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<ul> 
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  <li><a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/ElectraCloningProtocol"> Electra Cloning Protocol </a><br>
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  <li><a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/DNAhandling"> DNA Handling Protocol </a><br>
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  <li>Media:Zymo E. coli competent cells transformation protocol.pdf<br>
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  <li>Media:D4025i.pdf (Zyppy plasmid midiprep protocol)<br>
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  <li>Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf <br></ul>
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<p>General Documentation Information</p><ul>
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  <a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/ElectraCloningProtocol> Electra Cloning Protocol </a><br>
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    <li><a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/NotebookDocumentation">Real Vegan Cheese Notebook Documentation Guidelines</a><br>
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  <a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/DNAhandling> DNA Handling Protocol </a><br>
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     <li>Real Vegan Cheese Notebook Training Sign-off <br></ul>
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Media:Zymo E. coli competent cells transformation protocol.pdf<br>
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     Media:D4025i.pdf (Zyppy plasmid midiprep protocol)<br>
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    Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf <br>
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<br>
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<p>General Documentation Information
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</p>
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    Real Vegan Cheese Notebook Documentation Guidelines<br>
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    Real Vegan Cheese Notebook Training Sign-off <br>
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<p>June 2014
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<p>June 2014</p><ul>
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</p>
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     <li>CR_062214_humanKappaCasein_001 <br></ul></p>
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     CR_062214_humanKappaCasein_001 <br>
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<p>August 2014
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<p>August 2014</p><ul>
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     <li>CLONE_082514_humKappaCasein(Kex+)<br>
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     CLONE_082514_humKappaCasein(Kex+)<br>
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     <li>Growth Curve Experiment <br></ul></p>
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     Growth Curve Experiment <br>
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<p>September 2014</p><ul>
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September 2014
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     <li>Cloning 7 casein gBlocks, 12Sep2014 and on<br>
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</p>
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     <li>Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S<br>
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     Cloning 7 casein gBlocks, 12Sep2014 and on<br>
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     <li>Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014<br>
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     Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S<br>
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     <li>Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014<br></ul></p>
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     Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014<br>
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     Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014<br>  
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<p>
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<p>October 2014</p><ul>
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October 2014
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     <li>Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2<br>
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</p>
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     <li>Cloning human alpha casein S1, 04Oct2014 and on<br>
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     Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2<br>
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     <li>Submitting 4 repeat and 1 new casein in pD1214 plasmid for sequencing 10Oct2014<br></ul></p>
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     Cloning human alpha casein S1, 04Oct2014 and on<br>
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     Submitting 4 repeat and 1 new casein in pD1214 plasmid for sequencing 10Oct2014<br>  
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Revision as of 18:08, 16 October 2014



WELCOME TO iGEM 2014!

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Notebook

General Protocols


  • Electra Cloning Protocol
  • DNA Handling Protocol
  • Media:Zymo E. coli competent cells transformation protocol.pdf
  • Media:D4025i.pdf (Zyppy plasmid midiprep protocol)
  • Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf

General Documentation Information

June 2014

  • CR_062214_humanKappaCasein_001

August 2014

  • CLONE_082514_humKappaCasein(Kex+)
  • Growth Curve Experiment

September 2014

  • Cloning 7 casein gBlocks, 12Sep2014 and on
  • Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S
  • Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014
  • Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014

October 2014

  • Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2
  • Cloning human alpha casein S1, 04Oct2014 and on
  • Submitting 4 repeat and 1 new casein in pD1214 plasmid for sequencing 10Oct2014

Retrieved from "http://2014.igem.org/Team:SF_Bay_Area_DIYbio/Notebook"