Team:UESTC-China/result
From 2014.igem.org
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<strong>Fig.1</strong> Verification of backbones using digestion | <strong>Fig.1</strong> Verification of backbones using digestion | ||
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<p style="color:#1b1b1b;">In order to enhance the ability of tobacco to metabolize formaldehyde, we have successfully constructed 3 multigenge expression vectors, from vector <em>piGEM009</em> to vector <em>piGEM011</em>. We have verified all of them using digestion (Fig.3) and sequcencing.</p> | <p style="color:#1b1b1b;">In order to enhance the ability of tobacco to metabolize formaldehyde, we have successfully constructed 3 multigenge expression vectors, from vector <em>piGEM009</em> to vector <em>piGEM011</em>. We have verified all of them using digestion (Fig.3) and sequcencing.</p> | ||
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<p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:left; width:650px; color:#1b1b1b;"> | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:left; width:650px; color:#1b1b1b;"> | ||
<strong>Fig.3</strong> Verification of multi-gene expression vectors using digestion | <strong>Fig.3</strong> Verification of multi-gene expression vectors using digestion | ||
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M: DNA marker; | M: DNA marker; | ||
6: plasmid piGEM011 and it's digestion production | 6: plasmid piGEM011 and it's digestion production | ||
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<p style="color:#1b1b1b;">Tobacco was transformed essentially using the leaf disk co-cultivation protocol of Horsch. This protocol includes three stages, co-cultivation (Fig.4A), screening cultivation (Fig.4B) and rooting cultivation (Fig.4C). We have successfully transformed each vector into babacco. Table 1 is the statistical result of quantity of each transgenic line. And we have got PCR positive plantlet of every transgenic line.</p> | <p style="color:#1b1b1b;">Tobacco was transformed essentially using the leaf disk co-cultivation protocol of Horsch. This protocol includes three stages, co-cultivation (Fig.4A), screening cultivation (Fig.4B) and rooting cultivation (Fig.4C). We have successfully transformed each vector into babacco. Table 1 is the statistical result of quantity of each transgenic line. And we have got PCR positive plantlet of every transgenic line.</p> | ||
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<p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:left; width:650px; color:#1b1b1b;"> | <p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:left; width:650px; color:#1b1b1b;"> | ||
<strong>Fig.4</strong> | <strong>Fig.4</strong> |
Revision as of 01:14, 14 October 2014