Team:Saarland/Parts
From 2014.igem.org
Line 66: | Line 66: | ||
<b>Sequence:</b> <br> <!--EHER LINK ZU [http://parts.igem.org/Part:BBa_K1469002]--> | <b>Sequence:</b> <br> <!--EHER LINK ZU [http://parts.igem.org/Part:BBa_K1469002]--> | ||
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ATGACTAATATCACAGTAGCGGGTACTGGTTACGTAGGCTTAGTTACAGGTGTTTGTTTAGCTGAAATAGGGCATGAGGTAACATGTGTAGATAA | ATGACTAATATCACAGTAGCGGGTACTGGTTACGTAGGCTTAGTTACAGGTGTTTGTTTAGCTGAAATAGGGCATGAGGTAACATGTGTAGATAA | ||
TATTCAGGATAAAATTGATATTTTAAATGAAGGAATATCTCCTATCTTTGAACCTGGGCTTGATGAATTAATTATTAAAAACTCTGATGCTGGTA | TATTCAGGATAAAATTGATATTTTAAATGAAGGAATATCTCCTATCTTTGAACCTGGGCTTGATGAATTAATTATTAAAAACTCTGATGCTGGTA | ||
Line 81: | Line 83: | ||
TTGTTACAATCTCGATGAAGTTAAAAGAAGTGGTATCAATTATCAATCTATTGGTCGTCCTAATATAAATGGATACAAAGCTAAAATTAAAGCTA | TTGTTACAATCTCGATGAAGTTAAAAGAAGTGGTATCAATTATCAATCTATTGGTCGTCCTAATATAAATGGATACAAAGCTAAAATTAAAGCTA | ||
ATGTCTAA<br> | ATGTCTAA<br> | ||
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<b>Characterisation status:</b> | <b>Characterisation status:</b> | ||
Line 98: | Line 102: | ||
<b>Sequence: </b> <br> <!--EHER LINK ZU [http://parts.igem.org/Part:BBa_K1469003]--> | <b>Sequence: </b> <br> <!--EHER LINK ZU [http://parts.igem.org/Part:BBa_K1469003]--> | ||
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ATGGATGACAAATGGTTAGTTGGAGTTGATTTAGGCGGTACAACAATAAAAATGGCCTTTATTAATCATTATGGAGAAATCATTCACAAGTGGA | ATGGATGACAAATGGTTAGTTGGAGTTGATTTAGGCGGTACAACAATAAAAATGGCCTTTATTAATCATTATGGAGAAATCATTCACAAGTGGA | ||
AATTAATACGGATGTGAGCGAGCAAGGCCGTAAAATTCCAACGGATATTGCAAAAGCAATTGATAAAAAGTTAAACGATCTTGGAGAAGTAA | AATTAATACGGATGTGAGCGAGCAAGGCCGTAAAATTCCAACGGATATTGCAAAAGCAATTGATAAAAAGTTAAACGATCTTGGAGAAGTAA | ||
Line 109: | Line 115: | ||
AGAGATTATTTCAAACGTTTTGCATTTCCTCGCGTAGCGCAAGGTGCTGAACTAGCAATCGCTACTTTAGGAAACGATGCGGGAATTATTGGAG | AGAGATTATTTCAAACGTTTTGCATTTCCTCGCGTAGCGCAAGGTGCTGAACTAGCAATCGCTACTTTAGGAAACGATGCGGGAATTATTGGAG | ||
GAGCTTGGTTAGTTAAATCTTATTTTGAATAA<br> | GAGCTTGGTTAGTTAAATCTTATTTTGAATAA<br> | ||
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<b>Characterisation status:</b> | <b>Characterisation status:</b> | ||
Line 127: | Line 135: | ||
<b>Sequence:</b> <br> <!--EHER LINK ZU [http://parts.igem.org/Part:BBa_K1469004]--> | <b>Sequence:</b> <br> <!--EHER LINK ZU [http://parts.igem.org/Part:BBa_K1469004]--> | ||
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ATGAAAAAAGAAGAGCTACAAGCGATTATTTTTGATTTAGACGGTGTTATTGCAGATACGGTACATTTATATTATAAAGCAAATAAAAAAGT | ATGAAAAAAGAAGAGCTACAAGCGATTATTTTTGATTTAGACGGTGTTATTGCAGATACGGTACATTTATATTATAAAGCAAATAAAAAAGT | ||
AGCAGATCAACTTAACGTTTCATTTTCTGAAGAACTCAATCAGCAGCTGCAGGGGATCAGCCGAGTGAAAACTGTAGAGCTCATTGCTGCCC | AGCAGATCAACTTAACGTTTCATTTTCTGAAGAACTCAATCAGCAGCTGCAGGGGATCAGCCGAGTGAAAACTGTAGAGCTCATTGCTGCCC | ||
Line 135: | Line 145: | ||
GATATGGTTTCTATCGGCGTTGGTAAGCATCTGAAGGAAGAGGATATGAATTTTCACGTATATTCAACAGCTGATTTGCATTTAAAGACGAT | GATATGGTTTCTATCGGCGTTGGTAAGCATCTGAAGGAAGAGGATATGAATTTTCACGTATATTCAACAGCTGATTTGCATTTAAAGACGAT | ||
CAAAGAGGTTTTCGAAAAAACAAGAAAAGGAACAGATTATAACAGCGTGTATACAAAAAATTAA<br> | CAAAGAGGTTTTCGAAAAAACAAGAAAAGGAACAGATTATAACAGCGTGTATACAAAAAATTAA<br> | ||
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<b>Characterisation status:</b> | <b>Characterisation status:</b> | ||
Line 154: | Line 166: | ||
<b>Sequence:</b> <br> <!--EHER LINK ZU [http://parts.igem.org/Part:BBa_K1469005]--> | <b>Sequence:</b> <br> <!--EHER LINK ZU [http://parts.igem.org/Part:BBa_K1469005]--> | ||
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ATGACGATAAAAAAGGCAGTTATTCCAGTAGGCGGTTTAGGAACTCGATTTTTGCCAGCGACAAAAGCGCAGCCCAAAGAAATGCTGCCAAT | ATGACGATAAAAAAGGCAGTTATTCCAGTAGGCGGTTTAGGAACTCGATTTTTGCCAGCGACAAAAGCGCAGCCCAAAGAAATGCTGCCAAT | ||
TGTCGATAAGCCTGCTGTACAATATATTGTAGAAGAAGCTGTGAAATCAGGTATTGAAAGTATTATTTTTATTACAGGACGAAACAAAAAAT | TGTCGATAAGCCTGCTGTACAATATATTGTAGAAGAAGCTGTGAAATCAGGTATTGAAAGTATTATTTTTATTACAGGACGAAACAAAAAAT | ||
Line 164: | Line 178: | ||
AACTGGAAGGACAGCGCTTTGATATTGGAGATAAGGTAGGCTATATGAAAGCGATGGTAGAAGTTGCGTTAAAGCGCAGAGATTTACGTCAA | AACTGGAAGGACAGCGCTTTGATATTGGAGATAAGGTAGGCTATATGAAAGCGATGGTAGAAGTTGCGTTAAAGCGCAGAGATTTACGTCAA | ||
ACGTTTTTATCGTATTTGGAGGGAATTGTAGAAAAAGAACGAGCAAACAACTTCAGCTAA<br> | ACGTTTTTATCGTATTTGGAGGGAATTGTAGAAAAAGAACGAGCAAACAACTTCAGCTAA<br> | ||
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<b>Characterisation status:</b> | <b>Characterisation status:</b> | ||
Line 182: | Line 198: | ||
<b>Sequence:</b> <br> <!-- EHER LINK ZU [http://parts.igem.org/Part:BBa_K1469006]--> | <b>Sequence:</b> <br> <!-- EHER LINK ZU [http://parts.igem.org/Part:BBa_K1469006]--> | ||
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ATGACACATGTTCGTTTTGATTATTCAAACGCGTTATCATTTTTTGGTGAACATGAACTTACATACTTACGTGATGCCGTGAAAGTAGCTC | ATGACACATGTTCGTTTTGATTATTCAAACGCGTTATCATTTTTTGGTGAACATGAACTTACATACTTACGTGATGCCGTGAAAGTAGCTC | ||
ACCATTCAATTCATGAGAAAACAGGTGCTGGAAACGACTTTTTAGGATGGGTCGACCTTCCTACGGAGTATGACAAAGAAGAATTTGCACG | ACCATTCAATTCATGAGAAAACAGGTGCTGGAAACGACTTTTTAGGATGGGTCGACCTTCCTACGGAGTATGACAAAGAAGAATTTGCACG | ||
Line 197: | Line 215: | ||
ATGTTGTATATTTCTTCGAAAAAGCATGTGCAATCAGCGGCTATTTATTAGGCGTAAACCCATTCGATCAGCCGGGAGTAGAAGCATACAA | ATGTTGTATATTTCTTCGAAAAAGCATGTGCAATCAGCGGCTATTTATTAGGCGTAAACCCATTCGATCAGCCGGGAGTAGAAGCATACAA | ||
AGTAAATATGTTTGCTCTTCTTGGAAAACCTGGTTTTGAAGAGAAAAAAGCAGAATTAGAAAAGCGTTTGAAATAA<br> | AGTAAATATGTTTGCTCTTCTTGGAAAACCTGGTTTTGAAGAGAAAAAAGCAGAATTAGAAAAGCGTTTGAAATAA<br> | ||
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<b>Characterisation status:</b> | <b>Characterisation status:</b> | ||
Line 215: | Line 235: | ||
<b>Sequence:</b> <br> <!--EHER LINK ZU [http://parts.igem.org/Part:BBa_K1469007]--> | <b>Sequence:</b> <br> <!--EHER LINK ZU [http://parts.igem.org/Part:BBa_K1469007]--> | ||
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ATGTGCGGAATTGTAGGATATATTGGAACAGAAGATTCGAAAGAAATTTTATTACGTGGTCTTGAAAAGTTAGAATATCGTGGCTATGACT | ATGTGCGGAATTGTAGGATATATTGGAACAGAAGATTCGAAAGAAATTTTATTACGTGGTCTTGAAAAGTTAGAATATCGTGGCTATGACT | ||
CAGCGGGTATTGCAGTTGTTAACAATGAAGGCGTACACGTATTCAAAGAAAAAGGCCGTATTGCAGAATTGCGTCAGGCAGTAGATAACAG | CAGCGGGTATTGCAGTTGTTAACAATGAAGGCGTACACGTATTCAAAGAAAAAGGCCGTATTGCAGAATTGCGTCAGGCAGTAGATAACAG | ||
Line 235: | Line 257: | ||
TTAGAAACAGAAGAAGATCGCTACGTGATTCCGAAAGTTCACGAAACACTTTCACCACTAGCAGCGGTTATTCCTTTACAATTAATCTCTT | TTAGAAACAGAAGAAGATCGCTACGTGATTCCGAAAGTTCACGAAACACTTTCACCACTAGCAGCGGTTATTCCTTTACAATTAATCTCTT | ||
ACTACGCTGCATTGCACCGCGATTGCGATGTAGATAAACCACGTAACTTAGCTAAATCAGTTACTGTAGAATAA<br> | ACTACGCTGCATTGCACCGCGATTGCGATGTAGATAAACCACGTAACTTAGCTAAATCAGTTACTGTAGAATAA<br> | ||
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<b> Characterisation status:</b> | <b> Characterisation status:</b> | ||
Line 253: | Line 277: | ||
<b>Sequence:</b> <br> <!--EHER LINK ZU [http://parts.igem.org/Part:BBa_K1469008]--> | <b>Sequence:</b> <br> <!--EHER LINK ZU [http://parts.igem.org/Part:BBa_K1469008]--> | ||
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ATGGGTAAGTATTTTGGAACAGACGGAGTACGAGGAGTTGCCAATAGTGAATTAACTCCTGAGCTTGCTTTTAAAATTGGACGCTTAGGTG | ATGGGTAAGTATTTTGGAACAGACGGAGTACGAGGAGTTGCCAATAGTGAATTAACTCCTGAGCTTGCTTTTAAAATTGGACGCTTAGGTG | ||
GTTACGTTCTAACAAAAGATTCAGAGCGACCAAAAGTATTAATCGGACGCGATACACGCGTTTCTGGTCATATGTTAGAAGGAGCGCTTGT | GTTACGTTCTAACAAAAGATTCAGAGCGACCAAAAGTATTAATCGGACGCGATACACGCGTTTCTGGTCATATGTTAGAAGGAGCGCTTGT | ||
Line 268: | Line 294: | ||
AAGAAGTGGAAAAAGAAATGAACGGTAATGGCCGAGTACTTGTACGCCCTTCAGGAACAGAGCCGTTAGTACGTGTGATGGTTGAAGCACA | AAGAAGTGGAAAAAGAAATGAACGGTAATGGCCGAGTACTTGTACGCCCTTCAGGAACAGAGCCGTTAGTACGTGTGATGGTTGAAGCACA | ||
AACACAAGAAGAATGTGAAACTTATGTAACGCGTATCGTAGAAGTAGTAAAAGAAGAAATGGGCTTAGAGTAA<br> | AACACAAGAAGAATGTGAAACTTATGTAACGCGTATCGTAGAAGTAGTAAAAGAAGAAATGGGCTTAGAGTAA<br> | ||
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<br> | <br> | ||
<b>Characterisation status:</b> | <b>Characterisation status:</b> | ||
Line 286: | Line 314: | ||
<b>Sequence:</b> <br> <!--EHER LINK ZU [http://parts.igem.org/Part:BBa_K1469009]--> | <b>Sequence:</b> <br> <!--EHER LINK ZU [http://parts.igem.org/Part:BBa_K1469009]--> | ||
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ATGTCAAAAAGATATGCAGTCATATTGGCAGCTGGTCAGGGTACACGTATGAAGTCATCTTTATATAAAGTGTTACACCCTGTGTGTGGCAA | ATGTCAAAAAGATATGCAGTCATATTGGCAGCTGGTCAGGGTACACGTATGAAGTCATCTTTATATAAAGTGTTACACCCTGTGTGTGGCAA | ||
ACCGATGGTGCAACACGTAATTGATCAGTTATCACGTTTGGATTTAACAAGTTTAATTACAGTTGTAGGTCACGGTGCCGAAAAAGTGAAAT | ACCGATGGTGCAACACGTAATTGATCAGTTATCACGTTTGGATTTAACAAGTTTAATTACAGTTGTAGGTCACGGTGCCGAAAAAGTGAAAT | ||
Line 301: | Line 331: | ||
TGGGGCATTCGTAGGATGTAACTCAAACTTAATTGCACCTGTAACAATTGGTGAAGGCGCCTATGTTGCAGCTGGTTCAACCGTAACGGATG | TGGGGCATTCGTAGGATGTAACTCAAACTTAATTGCACCTGTAACAATTGGTGAAGGCGCCTATGTTGCAGCTGGTTCAACCGTAACGGATG | ||
ATGTGCCAGGAAAAGCATTATCAATTGCTCGTGCGAAACAAGTAAATAAAGAAAACTATGCTGAAAAGCTTGATATTAATAAAAAATCCTAA<br> | ATGTGCCAGGAAAAGCATTATCAATTGCTCGTGCGAAACAAGTAAATAAAGAAAACTATGCTGAAAAGCTTGATATTAATAAAAAATCCTAA<br> | ||
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<b>Characterisation status:</b> | <b>Characterisation status:</b> |
Revision as of 22:40, 13 October 2014
Parts:
Hyaluronic acid production
Number: [http://parts.igem.org/Part:BBa_K1469001 BBa_K1469001]
Description:
Hyaluronan synthase 2 gene of Heterocephalus glaber with codon adaption for expression in Bacillus megaterium. The enzyme catalyzes the linkage of UDP-D-glucuronic acid and UDP-N-acetyl-D-glucosamine to the linear high molecular mass hyaluronic acid chain and is also involved in its secretion.
Sequence:
ATGCACTGTGAACGTTTCTTATGTATTTTACGTATTATTGGTACAACATTATTCGGTGTATCTTTATTATTAGGTATTACAGCTGCTTACATTGTAGGTTACCAA
TTCATTCAAACAGATAACTACTACTTCTCTTTCGGTTTATACGGTGCTTTCTTAGCTTCTCACTTAATTATTCAATCTTTATTCGCTTTCTTAGAACACCGTAAA
ATGAAAAAATCTTTAGAAACACCAATTAAATTAAACAAAACAGTAGCTTTATGTATTGCTGCTTACCAAGAAGATCCAGATTACTTACGTAAATGTTTACAATCT
GTAAAACGTTTAACATACCCAGGTATTAAAGTAGTAATGGTAATTGATGGTAACTCTGATGATGATTTATACATGATGGATATTTTCTCTGAAGTAATGGGTCGT
GATAAATCTGCTACATACATTTGGAAAAACAACTTCCACGAAAAAGGTCCAGGTGAAACAGATGAATCTCACAAAGAATCTTCTCAACACGTAACACAATTAGTA
TTATCTTCTAAATCTGTATGTATTATGCAAAAATGGGGTGGTAAACGTGAAGTAATGTACACAGCGTTTCGTGCTCTCGGTCGTTCTGTTGATTACGTACAAGTA
TGTGATTCTGATACAATGTTAGATCCAGCTTCTTCTGTAGAAATGGTAAAAGTATTAGAAGAAGATCCAATGGTAGGTGGTGTAGGTGGTGATGTACAAATTTTA
AACAAATACGATTCTTGGATTTCTTTCTTATCTTCTGTACGTTACTGGATGGCTTTCAACATTGAACGTGCTTGTCAATCTTACTTCGGTTGTGTACAATGTATT
TCTGGTCCATTAGGTATGTACCGTAACTCTTTATTACACGAATTTGTAGAAGATTGGTACTCTCAAGAATTTATGGGTAACCAGTGCAGCTTCGGTGATGATCGT
CACTTAACAAACCGTGTATTATCTTTAGGTTACGCTACAAAATACACAGCTCGTTCTAAATGTTTAACAGAAACACCAATTGAATACTTACGTTGGTTAAACCAA
CAAACACGTTGGTCTAAATCTTACTTCCGTGAATGGTTATACAACGCTATGTGGTTCCACAAACACCACTTATGGATGACATACGAAGCGGTAATAACAGGCTTC
TTCCCATTCTTTTTAATTGCTACAGTAATTCAATTATTCTACCGTGGTAAAATTTGGAACATTTTATTATTCTTATTAACAGTACAATTAGTAGGTTTAATAAAA
TCTTCTTTCGCTTCTTGTTTACGTGGTAACATTGTAATGGTATTCATGTCTTTATACTCTGTATTATACATGTCTTCTTTATTACCAGCTAAAATGTTCGCTATT
GCTACAATTAACAAAGCTGGTTGGGGTACATCTGGTCGTAAAACAATTGTAGTAAACTTCATTGGTTTAATTCCAGTATCTGTATGGTTCACAATTTTATTAGGT
GGTGTAATTTTCACAATTTACAAAGAATCTAAAAAACCATTCTCTGAATCTAAACAAACAGTATTAATTGTAGGTACATTATTATACGCTTGTTACTGGGTAATG
TTATTAACATTATACGTAGTATTAATAAACAAATGTGGTCGTCGTAAAAAAGGTCAACAATACGATATGGTATTAGATGTATAA
Characterisation status:
- Part is user sequenced: no illegal restriction sites, no mutations
- Protein expression successful
- No catalytic activity probably due to misfolding and intracellular accumulation
Pathway engineering for optimsation of Hyaluronic acid production
Number: [http://parts.igem.org/Part:BBa_K1469002 BBa_K1469002]
Description:
UDP-glucose 6-dehydrogenase of Bacillus megaterium. The enzyme catalyzes the NAD dependent oxidation of UDP-glucose to UDP-glucuronic acid, a key precursor molecule for hyaluronic acid production.
Sequence:
ATGACTAATATCACAGTAGCGGGTACTGGTTACGTAGGCTTAGTTACAGGTGTTTGTTTAGCTGAAATAGGGCATGAGGTAACATGTGTAGATAA
TATTCAGGATAAAATTGATATTTTAAATGAAGGAATATCTCCTATCTTTGAACCTGGGCTTGATGAATTAATTATTAAAAACTCTGATGCTGGTA
AATTATTTTTCACAACTGATTATCAAAATACTTATGCAAAATCTGATGTTATTTTTATAGGTGTAGGAACACCTGAAAATGAAGATGGGTCTGCT
AATTTAAATTATGTTTATACAGTTGCACAACAAATAGCTAAATTTATTGAGCGAGATTGTCTTATTGTAGTAAAATCTACTGTTCCTATTGGAAC
AAATGAGAAAATAGAAAAATTAATTAAACAACATTTAAAAAATGATGTACAAGTTGAGGTAGCATCCAACCCTGAGTTTTTAGCACAAGGAACAG
CAGTGCGTGACACACTTTATGCATCACGTATTGTTATAGGAGTAGAAAGTGAAAGAGCAAGACAAATATTAACTGATATTTATCAACCTTTTCAT
CAACCTATCTTAACAATGAATCGTCGTAGTGCTGAGATGGTAAAGTATGCTTCAAATGATTTCTTAGCATTAAAAATATCATTTGTTAATGATAT
TGCTAACCTGTGTGAAGTAGTAGGGGCTAACATTGAAGATGTTACAAATGGTATGAGCTATGATCAACGTATTGGAGACAAGTTTTTAAATCCTG
GCATCGGTTACGGTGGTTCATGCTTCCCTAAAGATACTAAAGCGTTACATTGGTTGTCTGAAGAGGAAGGCTATGTATTACGTACCATAAAAGCA
GCTATAGAAGTAAATGAAAAACAAAAGTTTAAACTGATTAAAAAAGCAAGACAAGACTTTGCAACTTTCAAGGGTTTAAAAGTTGCTGTTTTAGG
TCTTACATTCAAGCCTGGTACCGATGACTTACGGGAGGCACCATCAATTCCCAATATTCGTTTATTATTAAATGAAGGTGCTGAAGTTCATGCAT
ATGATCCTGTAGGTGTAAACAATTTTAAGAAAATATATCCAACACAAGTGTATTATGAAGAAACAATTGAAAAAGCATTAAAAAATGCTGACATT
TGTTTTATATTTACAGAGTGGGAAGAAATAAAAAATATGAATTTAAACTTGTTTAACAAACAAATGAAATCTCCTTTTGTCTATGATGGACGTAA
TTGTTACAATCTCGATGAAGTTAAAAGAAGTGGTATCAATTATCAATCTATTGGTCGTCCTAATATAAATGGATACAAAGCTAAAATTAAAGCTA
ATGTCTAA
Characterisation status:
- Part is user sequenced: no illegal restriction sites, no mutations
- Protein expression successful
- characterisation of catalytic activity not possible because no HA production for comparison of HA yields after co expression with hyaluronan synthase 2.
Number: [http://parts.igem.org/Part:BBa_K1469003 BBa_K1469003]
Description:
Glucokinase of Bacillus megaterium. The enzyme catalyzes the phosphorylation reaction of glucose to glucose 6-phospahate.
Sequence:
ATGGATGACAAATGGTTAGTTGGAGTTGATTTAGGCGGTACAACAATAAAAATGGCCTTTATTAATCATTATGGAGAAATCATTCACAAGTGGA
AATTAATACGGATGTGAGCGAGCAAGGCCGTAAAATTCCAACGGATATTGCAAAAGCAATTGATAAAAAGTTAAACGATCTTGGAGAAGTAA
AATCAAGGTTAGTAGGAATTGGCATTGGTGCACCGGGGCCTGTCAACTTTGCAAACGGTTCGATTGAAGTAGCTGTCAATTTAGGTTGGGAA
AAATTCCCTATAAAAGATATCTTGGAAGTAGAAACTTCTCTTCCTGTTGTAGTAGACAATGATGCAAACATTGCAGCGATTGGAGAAATGTGGA
AGGGTGCTGGAGACGGAGCAAAAGATTTACTTTGCGTTACGCTTGGCACAGGCGTTGGCGGTGGCGTCATTGCAAACGGTGAAATTGTACAAGG
CGTAAATGGAGCCGCTGGTGAGATCGGGCACATTACTTCTATTCCTGAAGGCGGGGCACCGTGTAACTGCGGTAAAACCGGCTGTTTAGAAACC
ATTGCTTCAGCAACTGGAATTGTACGTTTAACAATGGAAGAATTAACGGAAACGGACAAACCAAGTGAGCTTCGCACAGTGTTAGAACAAAATG
GACAAGTTACATCTAAAGATGTATTTGATGCAGCTCGTTCAAAAGACGGGTTAGCTATGCATGTTGTAGATAAAGTTGCTTTTCATTTAGGTCT
AGCACTAGCAAACTCTGCTAATGCATTAAACCCTGAGAAGATCGTTCTAGGCGGCGGTGTGTCTCGTGCAGGCGAGGTATTACTTGCACCGGTA
AGAGATTATTTCAAACGTTTTGCATTTCCTCGCGTAGCGCAAGGTGCTGAACTAGCAATCGCTACTTTAGGAAACGATGCGGGAATTATTGGAG
GAGCTTGGTTAGTTAAATCTTATTTTGAATAA
Characterisation status:
- Part is user sequenced: no illegal restriction sites, no mutations
- Part is not RFC10 conform.
- As indicated on the vector map: GCGGCCGCTTCTAG of prefix and TACTAGTAGCGGCCG of suffix are deleted
- Use only after amplification with appropriate primers
Number: [http://parts.igem.org/Part:BBa_K1469004 BBa_K1469004]
Description:
Beta-phosphoglucomutase of Bacillus megaterium. The enzyme catalyzes the conversion of glucose 6-phospahate to glucose 1-phosphate.
Sequence:
ATGAAAAAAGAAGAGCTACAAGCGATTATTTTTGATTTAGACGGTGTTATTGCAGATACGGTACATTTATATTATAAAGCAAATAAAAAAGT
AGCAGATCAACTTAACGTTTCATTTTCTGAAGAACTCAATCAGCAGCTGCAGGGGATCAGCCGAGTGAAAACTGTAGAGCTCATTGCTGCCC
AAGGCAATGTACATCTGACAATGGAAAAAAAAGAACAGCTTGCTGATGAAAAGAATAAGCATTATCAAGCATTAATAAAGGAAATGACAAAG
GATCATCTACTTCCAGGAATACAATTGTTGTTAGAGGATTGTAAAAAACAAGGTATTAAAATGGCGATTGCTTCTTCTAGTTCAAATGCAAA
TACTGTAGTGGATGCGTTAGGAATACGTCCTTTTTTTGATTGCATTGTGGATGTGAGAAAAATAAAGAAGGGAAAACCAGACCCAGAAATAT
TTTTGACAGCTGCCCATCAATTAAAAGTAACACCCTATGCATGTGTGGCTATAGAAGATGGAGAGGCGGGTGTAAAAGCTATTAATCAAACA
GATATGGTTTCTATCGGCGTTGGTAAGCATCTGAAGGAAGAGGATATGAATTTTCACGTATATTCAACAGCTGATTTGCATTTAAAGACGAT
CAAAGAGGTTTTCGAAAAAACAAGAAAAGGAACAGATTATAACAGCGTGTATACAAAAAATTAA
Characterisation status:
- Part is user sequenced: no illegal restriction sites, no mutations
- Part is not RFC10 conform
- As indicated on the vector map: GCGGCCGCTTCTAG of prefix is deleted
- Use only after amplification with appropriate primers
Number: [http://parts.igem.org/Part:BBa_K1469005BBa_K1469005]
Description:
UTP--glucose-1-phosphate uridylyltransferase of Bacillus megaterium. The enzyme catalyzes the conversion of glucose 1-phospahate to UDP-glucose 1-phosphate.
Sequence:
ATGACGATAAAAAAGGCAGTTATTCCAGTAGGCGGTTTAGGAACTCGATTTTTGCCAGCGACAAAAGCGCAGCCCAAAGAAATGCTGCCAAT
TGTCGATAAGCCTGCTGTACAATATATTGTAGAAGAAGCTGTGAAATCAGGTATTGAAAGTATTATTTTTATTACAGGACGAAACAAAAAAT
CAATTGAAGACCATTTTGATAAATCAACTGAGTTAGAGCAAATGCTTGAAGAAAAACACAAGTTCCAAATGCTAAAAGAAGTACAAACGATC
TCATCAATGGCCAGCATTCACTATATTCGTCAAAAAGAGCCGTTAGGACTTGGTCATGCTATTCTTTGTGCCGAACAGTTTATTGGAGATGA
GCCGTTTGCCGTTCTTCTTGGAGACGATATTATGACTTCTGAAGAACCTGCGCTCAAACAGATAATCCAAGCGTATGAAACGACTAATCAAT
CAGTTATCGGAGTTCAAAAAGTGGATGTTGAAGAGGTATCAAAATACGGAATCATTCAGCCGAAAAATAAATCTGGCAGCCTTCATGAAGTG
AATGACTTGATTGAAAAACCATCGATAGAACAAGCTCCGTCAACTATTGCCGTTATGGGCCGCTATATTTTAAATCCTTCTATTTTTTCTTA
TCTAAAAACGATTGAAAGAGGGGTAGGAAATGAATTACAATTAACAGATGCACTGGGTGTGGTATGTGAAAAAGAGCGATTGTTTGCTCTTG
AACTGGAAGGACAGCGCTTTGATATTGGAGATAAGGTAGGCTATATGAAAGCGATGGTAGAAGTTGCGTTAAAGCGCAGAGATTTACGTCAA
ACGTTTTTATCGTATTTGGAGGGAATTGTAGAAAAAGAACGAGCAAACAACTTCAGCTAA
Characterisation status:
- Part is user sequenced: no illegal restriction sites, no mutations
- Part is not RFC10 conform
- As indicated on the vector map: GCGGCCGCTTCTAG of prefix and TACTAGTAGCGGCCG of suffix are deleted
- Use only after amplification with appropriate primers
Number: [http://parts.igem.org/Part:BBa_K1469006 BBa_K1469006]
Description:
Glucose-6-phosphate isomerase of Bacillus megaterium. The enzyme catalyzes the conversion of glucose 6-phospahate to fructose 6-phosphate.
Sequence:
ATGACACATGTTCGTTTTGATTATTCAAACGCGTTATCATTTTTTGGTGAACATGAACTTACATACTTACGTGATGCCGTGAAAGTAGCTC
ACCATTCAATTCATGAGAAAACAGGTGCTGGAAACGACTTTTTAGGATGGGTCGACCTTCCTACGGAGTATGACAAAGAAGAATTTGCACG
CATTCAAAAAAGTGCAGAAAAAATTAAATCTGATTCAGATGTATTAATTGTTATCGGTATTGGTGGATCTTACTTAGGTGCTCGTGCTGCT
ATTGAAATGCTTCAGCATTCATTCTATAACACATTATCAAAAGAACAGCGTAAAACTCCGCAAGTTATTTTTGCTGGTAACAACATCAGCT
CAACATACATGAAAGACTTAATGGATGTTTTACAAGATAAAGATTTCTCTATTAATGTTATTTCAAAATCTGGTACAACAACAGAACCGGC
GATTGCTTTCCGTATCTTCCGTAAATTATTAGAAGAGAAATACGGCAAAGAAGAAGCACGCAAGCGTATTTATGCAACAACAGATAAAGCG
CGCGGTGCATTAAAAACATTAGCGGACGAAGAAGGCTATGAAACATTTGTTATTCCAGATGATGTAGGCGGCCGTTATTCAGTATTAACAG
CGGTTGGCTTATTACCAATTGCCGCTGCGGGTCTTGATATCGAACAAATGATGAAAGGGGCAGCTGATGCTCAAGAAGCATTCTCATCATC
AGAGCTAGAAGAGAACCCAGCTTATCAATATGCTGTAGCTCGTAATGTGCTTTATAATAAAGGAAAAACAATTGAAATGCTGATTAACTAT
GAGCCAGCTCTTCAATACTTTGCTGAGTGGTGGAAACAGTTATTTGGCGAAAGTGAAGGAAAAGACCAAAAAGGTATTTACCCTTCATCTG
CTAACTTCTCAACGGATCTTCATTCTTTAGGTCAATATGTACAAGAAGGTCGCCGTGATATTTTTGAAACAATTGTACAAGTTCAAGATTC
TCGTCATGAGCTAACAATTGAAGAAGATGCAGCGGACTTAGATGGATTAAATTACTTAGCTGGTGAAACAGTTGACTTTGTAAATAAAAAA
GCGTTCCAAGGTACAATGCTTGCTCACACAGATGGAGGCGTTCCAAACTTAGTTGTGAACATTCCTGCGCTAGATGAGTATACGTTCGGAT
ATGTTGTATATTTCTTCGAAAAAGCATGTGCAATCAGCGGCTATTTATTAGGCGTAAACCCATTCGATCAGCCGGGAGTAGAAGCATACAA
AGTAAATATGTTTGCTCTTCTTGGAAAACCTGGTTTTGAAGAGAAAAAAGCAGAATTAGAAAAGCGTTTGAAATAA
Characterisation status:
- Part is user sequenced: no illegal restriction sites, no mutations
- Part is not RFC10 conform
- As indicated on the vector map: TACTAGTAGCGGCCG of suffix is deleted
- Use only after amplification with appropriate primers
Number: [http://parts.igem.org/Part:BBa_K1469007 BBa_K1469007]
Description:
Glucosamine--fructose-6-phosphate aminotransferase (isomerising) of Bacillus megaterium. The enzyme catalyzes the conversion of fructose 6-phosphate to glucosamine 6-phosphate.
Sequence:
ATGTGCGGAATTGTAGGATATATTGGAACAGAAGATTCGAAAGAAATTTTATTACGTGGTCTTGAAAAGTTAGAATATCGTGGCTATGACT
CAGCGGGTATTGCAGTTGTTAACAATGAAGGCGTACACGTATTCAAAGAAAAAGGCCGTATTGCAGAATTGCGTCAGGCAGTAGATAACAG
TGTTGTAGCACCAGCAGGTATCGGTCATACGCGTTGGGCAACTCATGGTGTACCAAGCCAAGAAAATGCTCACCCGCATCAAAGTACATCT
GGACGCTTCACACTTGTGCATAACGGTGTAATTGAAAACTATGCTATTTTGAAACGTGAATATTTACAAGATGTAACGTTCAAAAGCGAAA
CGGATACAGAAGTAATCGTTCAGTTAATTCAAAAAATCGCAGCAGAAGGCTTAGATGTAGAAGAAGCGTTCCGTAAGACAGTAAGCTTGCT
TCATGGTTCTTATGCACTAGCTCTTGTTGATAACAAAGATAAAAATACAATCTACGTAGCTAAAAACAAAAGCCCGCTTCTTGTAGGTGTT
GGTGAAGGATTTAACGTTGTAGCGAGTGATGCTATGGCAATGCTTCAAGTAACAGATCAGTACGTAGAATTAATGGATAAAGAAACAGTAA
TTGTTACAAAAGAAGGCGTAACAATTAAAACGCTTGATGGTGAAGTGGTAGAAAGAGCACCTTATACAGCAGAGCTTGATGCAAGTGATAT
TGAAAAAGGTACGTACCCTCACTATATGTTAAAAGAAATTGACGAGCAGCCTTTAGTTGTTCGTAAAATCATTCAAGAGTATCAAAATGAG
AACAATGAACTGCACATTGATGCAGACATTTTAGGAGCAATGGATGAAGCAGACCGCATCTATATCGTAGCATGTGGAACAAGCTATCATG
CAGGCCTAGTAGGTAAACAATTCATTGAAACGTGGGCGAAGGTTCCAGTAGAAGTGCACGTAGCAAGTGAATTTTCTTATAACATGCCGCT
TTTATCTGAAAAGCCATTGTTTATCTTTATTTCTCAAAGTGGAGAAACAGCTGACAGCCGTGCAGTATTAGTTCAAATTAAAGAACTAGGT
TACAAAGCGTTAACAATCACAAACGTTCCGGGATCAACATTATCTCGTGAATCTGATTATACATTATTACTTCATGCAGGTCCGGAAATTG
CTGTAGCATCAACAAAAGCTTATACAGCTCAATTAGCAGTGCTATCAATTCTAGCGGCTGTAACAGCAAAAACACGCGGCATTGAATTAGA
ATTTGATTTAGTTCAAGAGTTAGCAATCGTAGCAAACACAATGGAAGTACTTTGCGACGACAAAGAAGAAATGGAAAGCATTGCTCTTCAA
TTCTTAGCAACAACTCGCAACGCATTCTTTATTGGACGTTCTGTCGATTATTATGTAGGTTTAGAAGGTGCGTTAAAACTAAAAGAAATCT
CTTACATCCAAGCAGAAGGATTTGCTGGAGGAGAGCTTAAGCACGGAACAATCGCTTTAATTGAAAACAATACGCCAATTATTGCATTAGC
AACACAAGAGCATGTAAACTTAAGCATCCGTGGTAACGTAAAAGAAGTAGTAGCACGTGGAGCTAATCCTTGTATCATTTCAATGAAAGGA
TTAGAAACAGAAGAAGATCGCTACGTGATTCCGAAAGTTCACGAAACACTTTCACCACTAGCAGCGGTTATTCCTTTACAATTAATCTCTT
ACTACGCTGCATTGCACCGCGATTGCGATGTAGATAAACCACGTAACTTAGCTAAATCAGTTACTGTAGAATAA
Characterisation status:
- Part is user sequenced: no illegal restriction sites, no mutations
- Part is not RFC10 conform
- As indicated on the vector map: TACTAGTAGCGGCCG of suffix is deleted
- Use only after amplification with appropriate primers
Number: [http://parts.igem.org/Part:BBa_K1469008 BBa_K1469008]
Description:
Phosphoglucosamine mutase of Bacillus megaterium. The enzyme catalyzes the conversion of glucosamine 6-phosphate to glucosamine 1-phosphate.
Sequence:
ATGGGTAAGTATTTTGGAACAGACGGAGTACGAGGAGTTGCCAATAGTGAATTAACTCCTGAGCTTGCTTTTAAAATTGGACGCTTAGGTG
GTTACGTTCTAACAAAAGATTCAGAGCGACCAAAAGTATTAATCGGACGCGATACACGCGTTTCTGGTCATATGTTAGAAGGAGCGCTTGT
TGCAGGATTGCTTTCAATTGGAGCAGAAGTTATGCGACTAGGTGTTATTTCAACTCCAGGGGTAGCGTATTTAACAAAAGCACTTGGCGCA
CAAGCAGGAGTCATGATTTCTGCTTCGCATAACCCAGTTCAAGATAACGGAATTAAATTCTTTGGTCCAGATGGATTTAAACTTTCAGATG
CCCAAGAGAATGAAATTGAAGCGTTAATGGATAGTGACACAGATCAATTACCAAGACCTGTTGGGGGAGACTTAGGTCAGGTAAATGACTA
TTTCGAAGGTGGACAAAAATATCTTCAATACTTAAAACAAACGGTTGATGAAGACTTCTCAGGTATTCATGTGGCATTAGACTGTGCGCAC
GGGGCAACGTCATCGTTAGCAGCACATTTATTTGCAGATCTCGATGCAGATATCTCTACAATGGGGGCATCTCCAAACGGATTGAACATCA
ATGACGGTGTAGGATCTACACATCCGGAAGCATTAAGTGCCTTCTTAAAAGAAAAAGGAGCGGATGTTGGATTAGCATTTGATGGAGACGG
AGATCGTTTAATCGCAATCGACGAAAAAGGCGAGATTGTAGACGGTGATCAAATTATGTTCATTTGTGCAAAATACTTATATGCACAAGGT
CGTTTAAAGAAAAACACGCTTGTTTCTACCGTTATGAGTAACTTAGGCTTCTATAAAGCACTAGAAGCTAGCGGGATTGAAAGTGCACAAA
CTGGTGTAGGAGACCGTTATGTAGTAGAAGAAATGAAAGCTAATCAATATAACCTAGGTGGAGAACAGTCAGGTCATATTATTTTCTTAGA
TTACAACACAACAGGTGACGGTATGCTTTCAGCTATTCAGCTTGTAAACATTATGCAAGCAACGAAAAAGCCTTTATCAGAATTAGCAGCT
GAAATGAAGAAATATCCTCAGCTTCTTGTAAATGTAAAAGTAACAGATAAGCATCATGTTACAGATAACGAGAAGGTAAAAGTGGTTATTG
AAGAAGTGGAAAAAGAAATGAACGGTAATGGCCGAGTACTTGTACGCCCTTCAGGAACAGAGCCGTTAGTACGTGTGATGGTTGAAGCACA
AACACAAGAAGAATGTGAAACTTATGTAACGCGTATCGTAGAAGTAGTAAAAGAAGAAATGGGCTTAGAGTAA
Characterisation status:
- Part is user sequenced: no illegal restriction sites, no mutations
- Part is not RFC10 conform
- As indicated on the vector map: GCGGCCGCTTCTAG of prefix and TACTAGTAGCGGCCG of suffix are deleted
- Use only after amplification with appropriate primers
Number: [http://parts.igem.org/Part:BBa_K1469009 BBa_K1469009]
Description:
UDP-N-acetylglucosamine pyrophosphorylase of Bacillus megaterium. The enzyme is bifunctional and catalyzes the conversion of glucosamine 1-phosphate to N-acetyl glucosamine 1-phosphate as well as the conversion of N-acetyl glucosamine 1-phosphate to UDP-N-acetyl glucosamine, a key precursor molecule for hyaluronic acid production.
Sequence:
ATGTCAAAAAGATATGCAGTCATATTGGCAGCTGGTCAGGGTACACGTATGAAGTCATCTTTATATAAAGTGTTACACCCTGTGTGTGGCAA
ACCGATGGTGCAACACGTAATTGATCAGTTATCACGTTTGGATTTAACAAGTTTAATTACAGTTGTAGGTCACGGTGCCGAAAAAGTGAAAT
CACATGTTGGAGATAAAAGCTTATTTGCTTTACAAGAAGAGCAGTTAGGAACAGCTCATGCAGTAATGCAAGCAGAATCTTTACTTGCACAT
GAAAAAGGTACGACAATTGTTGTATGTGGAGATACTCCGCTTATTACAGCTGAAACAATTGAATCTTTATTAAAGCATCATGAAGCAACAAA
TGCAATGGCAACTATTTTAACAGCTCATGCAGAAGATCCAACAGGATATGGACGCATCATTCGAAATGAACAAGGGTCTGTTGAAAAGATTG
TGGAGCATAAAGATGCCACAGAACAAGAGCGTCTTGTACAGGAAATTAATACTGGTACGTATTGTTTTGATAATGAGAAATTATTTGAGACA
CTTTCAAAAGTTTCAAATGATAATGTACAGGGAGAGTATTATTTACCTGATGTTATCGAAATTCTAAAAAATGAAGGTCAAATTATTTCTGC
ATATCAAACATCCGATTTTGCTGAAACATTGGGCGTTAATGATCGATTTGCTCTGTCTCAAGCAGAAGAAACAATGAAAAAGCGCATCAACA
AGCAGCATATGTTAAATGGAGTGACGATTGTGGATCCTTCTAACACGTACATTTCAGCAGATGCTGTCATTGGCAGAGATACGGTTATTTAT
CCTGGTACGGTTATTCAAGGAACAGTTGTAATTGGAGAGAATTGTGAAGTAGGACCTAACAGTGAAATTAAAGACTGCAAAATAGGTAATAA
TACTTCTATTCGCCATTCTGTTGCTCACGATAGTGAAATTGGTCACGAAGTCACAATTGGCCCATTTGCACATATTCGTCCTCAATCATTAA
TTGGAGATGAAGTGCGCGTAGGAAACTTTGTAGAAATTAAAAAAGCATCTTTTGGCAAAGGTAGCAAAGCTTCTCATTTAAGTTATATTGGA
GACGCAGAGGTCGGAAAAGGTGTAAACTTAGGGTGCGGATCTATTACAGTTAACTACGATGGGAAAAATAAATTTTTAACAAAGATTGAAGA
TGGGGCATTCGTAGGATGTAACTCAAACTTAATTGCACCTGTAACAATTGGTGAAGGCGCCTATGTTGCAGCTGGTTCAACCGTAACGGATG
ATGTGCCAGGAAAAGCATTATCAATTGCTCGTGCGAAACAAGTAAATAAAGAAAACTATGCTGAAAAGCTTGATATTAATAAAAAATCCTAA
Characterisation status:
- Part is user sequenced: no illegal restriction sites, no mutations
- Part is not RFC10 conform
- As indicated on the vector map: GCGGCCGCTTCTAG of prefix and TACTAGTAGCGGCCG of suffix are deleted
- Use only after amplification with appropriate primers
Impressum/Copyright