Team:Caltech/Notebook

From 2014.igem.org

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<ul><li>Formulated plasmid design for the response regulation subproject</li>
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<ul><li>Formulated plasmid design (pAA001) for the response regulation subproject</li>
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     <li>PCR plasmid backbone and sfGFP to create constructs with the proper overhangs for Gibson assembly</li>
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     <li>PCR run on plasmid backbone and sfGFP to create constructs with the proper overhangs for Gibson assembly</li>
     <li>Gel electrophoresis for confirmation of PCR products' length</li>
     <li>Gel electrophoresis for confirmation of PCR products' length</li>
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Revision as of 06:56, 25 June 2014



WELCOME TO iGEM 2014!

Your team has been approved and you are ready to start the iGEM season!
On this page you can document your project, introduce your team members, document your progress
and share your iGEM experience with the rest of the world!


Click here to edit this page!

Home Team Official Team Profile Project Parts Modeling Notebook Safety Attributions

Notebook

Apparently there's a calendar feature to take care of this too???

Week One

Tuesday, 6/17/14
  • Get acquainted with summer logistics and lab basics
  • Clean up lab space, stock lab supplies
 We got absolutely no results today!!!

Wednesday, 6/18/14
  • Continue to discuss project and clean up lab space
  • Designed PCR primers for combinatorial promoters subproject
 We got absolutely no results today!!!

Thursday, 6/19/14
  • Formulated plasmid design (pAA001) for the response regulation subproject
  • PCR run on plasmid backbone and sfGFP to create constructs with the proper overhangs for Gibson assembly
  • Gel electrophoresis for confirmation of PCR products' length
 With the exception of the lacI gene, PCR products were confirmed via gel electrophoresis

Friday, 6/20/14
  • Purification of PCR products created yesterday
  • Gibson assembly of purified products
  • Transformation of Gibson-assembled plasmids into JM109 E. coli
  • Meeting with Prof. Murray to discuss give an update on the project
 Colonies were found and picked after transformation and incubation.

Week Two

Monday, 6/23/14
  • Discussion of fallout from Friday's meeting, paper reading
  • Colony PCR and Minipreps of liquid cultures of colonies picked over the weekend
  • TXTL workshop starts today

Tuesday, 6/24/14
  • Discussed alternatives to current ComQXPA system, including 2-cell system (not all in 1 cell)
  • Gibson assembly of another combinatorial promoter using pKS001 backbone, lacI geneblock, ____ promoter geneblock, and sfGFP geneblock.
  • Transformation of Gibson constructs into JM109 cells
  • Linear combinatorial constructs created via PCR of pAA001 (minipreps) to be tested in TXTL

Wednesday, 6/25/14

Thursday, 6/26/14
Colonies were found and picked after transformation and incubation.


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