Team:UESTC-China/Modeling2

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  <h1 style="color:#1b1b1b; position:relative; left:0px; padding:15 5px; font-size:35px; font-family: calibri, arial, helvetica, sans-serif; font-weight: bold;font-style: Italic; text-align:center; width:1140px;">Folate-independent pathway</h1>
  <h1 style="color:#1b1b1b; position:relative; left:0px; padding:15 5px; font-size:35px; font-family: calibri, arial, helvetica, sans-serif; font-weight: bold;font-style: Italic; text-align:center; width:1140px;">Folate-independent pathway</h1>
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  <h1 class="SectionTitles" style="text-align:left; width:500px;">Mathematical Principles</h1>
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  <p style="color:#1b1b1b;">View previous presentation in the modeling of photosynthetic HCHO assimilation pathway:</p>
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  <a href="https://2014.igem.org/Team:UESTC-China/Modeling1" class="button">Photosynthetic HCHO assimilation pathway (HPS/PHI)</a>
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  <h1 class="SectionTitles" style="width:500px;"> Folate-independent pathway</h1>
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<p style="color:#1b1b1b;">The metabolism of folate-independent pathway was shown on Fig.1.</p>
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<p><img style="width:50%; margin-left:15px;" src="https://static.igem.org/mediawiki/2014/5/5c/MFig-4.jpg"></p>
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<p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: Italic; text-align:left; width:680px; color:#1b1b1b;"><strong>Fig.1</strong> Schematic diagram offormaldehyde metabolic pathways in plant. SMM cycle, S-methylmethionine cycle</p>
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<p style="color:#1b1b1b;">Simplify the system (Fig.2), consist of the input of HCHO and the recycle of HCOOH:</p>
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<p><img style="width:50%; margin-left:15px;" src="https://static.igem.org/mediawiki/2014/c/ce/MFig-5.jpg"></p>
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<p style="position:relative; left:0px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: Italic; text-align:left; width:680px; color:#1b1b1b;"><strong>Fig.2</strong> A simplified version of folate-independent pathway. FTS, 10-Formyl-THF synthetase. </p>
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<p style="color:#1b1b1b;">Some research indicated that the activity of FTS in tobacco was low and we ignored this pathway in our system. The chemical reactions of this formaldehyde metabolism are shown below.</p>
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<p style="color:#1b1b1b;"><img style="width:65%; margin-left:15px;" src="https://static.igem.org/mediawiki/2014/6/6b/M11.gif"><em style="position:absolute; right:150px; top: 55px;">(1)</em></p>
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<p style="color:#1b1b1b;">where parameter <i>a</i> is a constant which means the proportion of HCOOH into the calvin cycle. The dynamic equation group of the reaction system can be obtained:</p>
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<p style="color:#1b1b1b;"><img style="width:50%; margin-left:15px;" src="https://static.igem.org/mediawiki/2014/4/43/M12.gif"><em style="position:absolute; right:150px; top: 55px;">(2)</em></p>
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<h1 class="SectionTitles" style="text-align:left; width:500px;">Results</h1>
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<p style="color:#1b1b1b;">By changing the value of the parameters (<i>k0, k1</i> … ), we obtained the relationship (Fig.3) between the concentration of different components (HCOOH and HCHO) versus the time.</p>
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<p><img style="width:80%; position:relative; left:-30px;" src="https://static.igem.org/mediawiki/2014/6/6d/MFig-6.jpg"></p>
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<p style="position:relative; left:30px; padding:15 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: Italic; text-align:left; width:680px; color:#1b1b1b;"><strong>Fig.3</strong> The diagram of concentration versus time.</p>
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<p style="color:#1b1b1b;">We found that the components tend to be the steady state when time goes by. Means that when the formaldehyde into the plant cell, the original steady state was broken, but after a period of time, the cells will restore homeostasis.</p>
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<h1 class="SectionTitles" style="text-align:left; width:500px;">Link to other modeling</h1>
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<a href="https://2014.igem.org/Team:UESTC-China/Modeling1" class="button">Photosynthetic HCHO assimilation pathway (HPS/PHI)</a>
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<a href="https://2014.igem.org/Team:UESTC-China/Modeling3" class="button">Modeling of stoma (AtAHA2)</a>
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Revision as of 10:58, 13 October 2014

UESTC-China