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Team:Aachen/Collaborations/Heidelberg
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{{Team:Aachen/Header}} | {{Team:Aachen/Header}} | ||
| - | = Characterization of pSBX-vectors [[Team:Heidelberg|Team Heidelberg]] = | + | = '''The project X-press''': Characterization of pSBX-vectors made by [[Team:Heidelberg|Team Heidelberg]] = |
The iGEM team Heidelberg constructed a set of expression vectors that contain a T7 promoter prior to the BioBrick prefix. This way RBS-CDS parts can be cloned into the vectors by standard BioBrick assembly and then expressed in BL21(DE3) by induction with IPTG. | The iGEM team Heidelberg constructed a set of expression vectors that contain a T7 promoter prior to the BioBrick prefix. This way RBS-CDS parts can be cloned into the vectors by standard BioBrick assembly and then expressed in BL21(DE3) by induction with IPTG. | ||
| + | In collaboration with the iGEM Team Heidelberg the iGEM Team Aachen tested 8 of these vectors for their expression capabilities. | ||
| + | == The Team Heidelberg expression vectors == | ||
| + | |||
| + | These are the tested expression backbones: | ||
| + | |||
| + | {| class="wikitable" | ||
| + | ! Plasmid !! Backbone !! Insert !! Length vector !! Length Insert !! Resistance | ||
| + | | pSBX1A3-BBa_13507 (high copy) || pSBX1A3 || BBa_13507 || 3067 bp || 1226 bp || 50 mg/ml Ampicilin | ||
| + | |- | ||
| + | | pSBX1C3-BBa_13507 (high copy) || pSBX1C3 || BBa_13507 || 2982 bp || 1226 bp || 35 mg/ml Chloramphenicol | ||
| + | |- | ||
| + | |} | ||
{{Team:Aachen/Footer}} | {{Team:Aachen/Footer}} | ||
Revision as of 14:39, 12 October 2014
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