Team:Bielefeld-CeBiTec/Notebook/StrainsAndConstructs

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<td width="30%" id=""> <i>E.coli</i> KRX &Delta;dcuB::oprF</td>
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<td width="30%" id="KRXdeltadcuB"> <i>E.coli</i> KRX &Delta;dcuB::oprF</td>
<td width="40%">[F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase, &Delta;dcuB::oprF </td>
<td width="40%">[F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase, &Delta;dcuB::oprF </td>
<td width="30%"> <a href="http://parts.igem.org/Part:BBa_K1172507">BBa_K1172507</a></td>
<td width="30%"> <a href="http://parts.igem.org/Part:BBa_K1172507">BBa_K1172507</a></td>

Revision as of 00:25, 14 October 2014


Strains


Name Properties / Genotype BioBrick / Reference
General
Escherichia coli KRX [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase Promega
E. coli DH5α F-φ80lacZ∆M15∆(lacZYA-argF) U169recA1endA1hsdR17(rk-, mk+)gal-phoAsupE44λ-thi-1gyrA96relA1 invitrogen
Module 1
E.coli KRX ΔdcuB::oprF [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase, ΔdcuB::oprF BBa_K1172507
JW4084-1 ΔdcuB749::kan Baba et al., 2006
JW0506-1 ΔfdrA730::kan Baba et al., 2006
Actinobacillus succinogenes 130Z wild type Guettler et al., 1999
Geobacter sulfurreducens PCA wild type Viulu et al., 2013
Module 2
E.coli BL21 F– ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) NEB
Module 3
JW1228-1 ΔadhE748::kan Baba et al., 2006
Biosafety
E. coli KRX Δalr [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase, Δalr
E. coli KRX ΔdadX [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase, ΔdadX
E. coli KRX Δalr ΔdadX [F ́,traD36,ΔompP,proA+B+,lacIq,Δ(lacZ)M15]ΔompT,endA1,recA1,gyrA96(Nalr),thi-1,hsdR17 (rK–, mK+), e14–(McrA–),relA1,supE44,Δ(lac-proAB),Δ(rhaBAD)::T7 RNA polymerase, ΔdadX, Δalr
E. coli DH5α Δalr F-φ80lacZ∆M15∆(lacZYA-argF) U169recA1endA1hsdR17(rk-, mk+)gal-phoAsupE44λ-thi-1gyrA96relA1 Δalr
E. coli DH5α ΔdadX F-φ80lacZ∆M15∆(lacZYA-argF) U169recA1endA1hsdR17(rk-, mk+)gal-phoAsupE44λ-thi-1gyrA96relA1 ΔdadX
E. coli DH5α Δalr ΔdadX F-φ80lacZ∆M15∆(lacZYA-argF) U169recA1endA1hsdR17(rk-, mk+)gal-phoAsupE44λ-thi-1gyrA96relA1 Δalr ΔdadX
References

Constructs


Name Properties BioBrick / Reference
General
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Module 1
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Module 2
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Module 3
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Biosafety
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References
  • paper