Team:Hong Kong HKUST/data

From 2014.igem.org

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<ul>
<ul>
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<li> <b>SigmaX, <a href= "http://parts.igem.org/Part:BBa_K1379004"> BBa_K1379004</a></b>: gene sequence of competence protein comX which produces &sigma;<sup>x</sup> factor. &sigma;<sup>x</sup> factor induces promoter containing combox sequence (P<sub>celA</sub> and P<sub>helicase</sub>).
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<li> <b>&sigma;<sup>x</sup>, <a href= "http://parts.igem.org/Part:BBa_K1379004">BBa_K1379004</a></b>: gene sequence of competence protein ComX which produces &sigma;<sup>x</sup> factor. &sigma;<sup>x</sup> factor induces promoter containing Com-Box sequence (P<sub>celA</sub> and P<sub>comFA</sub>).
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<li> <b>P<sub>celA</sub> promoter, <a href= "http://parts.igem.org/Part:BBa_K1379000"> BBa_K1379000</a></b>: a &sigma;<sup>x</sup>-inducible promoter (containing combox sequence) which initiates the transcription of competence CelA protein.
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<li> <b>P<sub>celA</sub> promoter, <a href= "http://parts.igem.org/Part:BBa_K1379000"> BBa_K1379000</a></b>: a &sigma;<sup>x</sup>-inducible promoter (containing Com-Box sequence) which initiates the transcription of competence CelA protein.
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<li> <b>P<sub>helicase</sub> promoter, <a href= "http://parts.igem.org/Part:BBa_K1379001"> BBa_K1379001</a></b>: a &sigma;<sup>x</sup>-inducible promoter (containing combox sequence) which initiates the transcription of helicase protein.
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<li> <b>P<sub>comFA</sub> promoter, <a href= "http://parts.igem.org/Part:BBa_K1379001"> BBa_K1379001</a></b>: a &sigma;<sup>x</sup>-inducible promoter (containing Com-Box sequence) which initiates the transcription of ComFA protein.
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<li><b>Strong promoter, strong RBS combination, <a href= "http://parts.igem.org/Part:BBa_K880005"> BBa_K880005 </a></b>(Team Michigan iGEM, 2012): combination of strong constitutive promoter (<a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_J23100"> BBa_J23100</a>) and strong RBS (<a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034"> BBa_B0034 </a>) was used as a backbone for a very high level of expression of ComX protein (&sigma;<sup>x</sup> factor).
<li><b>Strong promoter, strong RBS combination, <a href= "http://parts.igem.org/Part:BBa_K880005"> BBa_K880005 </a></b>(Team Michigan iGEM, 2012): combination of strong constitutive promoter (<a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_J23100"> BBa_J23100</a>) and strong RBS (<a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034"> BBa_B0034 </a>) was used as a backbone for a very high level of expression of ComX protein (&sigma;<sup>x</sup> factor).
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<li><b>GFP generator</b>, <a href= "http://parts.igem.org/Part:BBa_E0240"> BBa_E0240 </a></b>(Antiquity Boston University iGEM, 2004): green fluorescent protein reporter (<a href= "http://parts.igem.org/Part:BBa_E0240"> BBa_E0040 </a>) with RBS (<a href= "http://parts.igem.org/Part:BBa_B0032"> BBa_B0032</a>) and double terminator (<a href= "http://parts.igem.org/Part:BBa_B0015">  BBa_B0015</a>) was used as a reporter for characterization of P<sub>celA</sub> and P<sub>helicase</sub> promoters.
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<li><b>GFP generator</b>, <a href= "http://parts.igem.org/Part:BBa_E0240"> BBa_E0240 </a></b>(Antiquity Boston University iGEM, 2004): green fluorescent protein reporter (<a href= "http://parts.igem.org/Part:BBa_E0240"> BBa_E0040 </a>) with RBS (<a href= "http://parts.igem.org/Part:BBa_B0032"> BBa_B0032</a>) and double terminator (<a href= "http://parts.igem.org/Part:BBa_B0015">  BBa_B0015</a>) was used as a reporter for characterization of P<sub>celA</sub> and P<sub>comFA</sub> promoters.
</ul>
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<ul>
<ul>
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<li> <b>SigmaX Generator, <a href="http://parts.igem.org/Part:BBa_K1379006"> BBa_K1379006</a></b>: <i>comX</i> gene sequence was assembled with constitutive promoter, RBS and double terminator so ComX protein is produced constitutively.
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<li> <b>&sigma;<sup>x</sup> Generator, <a href="http://parts.igem.org/Part:BBa_K1379006"> BBa_K1379006</a></b>: <i>comX</i> gene sequence was assembled with constitutive promoter, RBS and double terminator so ComX protein is produced constitutively.
<li> <b> P<sub>celA</sub>-GFP Generator, <a href="http://parts.igem.org/Part:BBa_K1379002"> BBa_K1379002</a></b>: &sigma;<sup>x</sup>-inducible P<sub>celA</sub> promoter was assembled with GFP generator.
<li> <b> P<sub>celA</sub>-GFP Generator, <a href="http://parts.igem.org/Part:BBa_K1379002"> BBa_K1379002</a></b>: &sigma;<sup>x</sup>-inducible P<sub>celA</sub> promoter was assembled with GFP generator.
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<li> <b>P<sub>helicase</sub>-GFP Generator, <a href= "http://parts.igem.org/Part:BBa_K1379003"> BBa_K1379003</a></b>: &sigma;<sup>x</sup>-inducible P<sub>helicase</sub> promoter was assembled with GFP generator.
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<li> <b>P<sub>comFA</sub>-GFP Generator, <a href= "http://parts.igem.org/Part:BBa_K1379003"> BBa_K1379003</a></b>: &sigma;<sup>x</sup>-inducible P<sub>comFA</sub> promoter was assembled with GFP generator.
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<li> <b>SigmaX Generator, P<sub>celA</sub>-GFP Generator, <a href= "http://parts.igem.org/Part:BBa_K1379005"> BBa_K1379005</a></b>: &sigma;<sup>x</sup>-generator was assembled with P<sub>celA</sub>-GFP generator. Following the assemblage of both constructs, GFP was successfully expressed.
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<li> <b>&sigma;<sup>x</sup> Generator, P<sub>celA</sub>-GFP Generator, <a href= "http://parts.igem.org/Part:BBa_K1379005"> BBa_K1379005</a></b>: &sigma;<sup>x</sup>-generator was assembled with P<sub>celA</sub>-GFP generator. Following the assemblage of both constructs, GFP was successfully expressed.
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<li> <b>SigmaX Generator, P<sub>helicase</suB>-GFP Generator, <a href="http://parts.igem.org/Part:BBa_K1379007"> BBa_K1379007</a></b>: &sigma;<sup>x</sup>-generator was assembled with P<sub>helicase</sub>-GFP generator. Following the assemblage of both constructs, GFP was successfully expressed.  
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<li> <b>&sigma;<sup>x</sup> Generator, P<sub>comFA</suB>-GFP Generator, <a href="http://parts.igem.org/Part:BBa_K1379007"> BBa_K1379007</a></b>: &sigma;<sup>x</sup>-generator was assembled with P<sub>comFA</sub>-GFP generator. Following the assemblage of both constructs, GFP was successfully expressed.  
</ul>
</ul>
</p>
</p>

Revision as of 13:44, 10 October 2014




Pneumosensor Data Page

This page shows all the parts we have made or used in our whole project. Click on the links below to see our constructs on the Registry of Standard Biological Parts in detail. Click here to access our result page for an overview of our achievements with those parts.

How our system works:
[Diagram with Biobrick Code]

Data For Our Favorite New Parts:

  • σx, BBa_K1379004: gene sequence of competence protein ComX which produces σx factor. σx factor induces promoter containing Com-Box sequence (PcelA and PcomFA).
  • PcelA promoter, BBa_K1379000: a σx-inducible promoter (containing Com-Box sequence) which initiates the transcription of competence CelA protein.
  • PcomFA promoter, BBa_K1379001: a σx-inducible promoter (containing Com-Box sequence) which initiates the transcription of ComFA protein.


Data for Pre-existing Parts:

  • Strong promoter, strong RBS combination, BBa_K880005 (Team Michigan iGEM, 2012): combination of strong constitutive promoter ( BBa_J23100) and strong RBS ( BBa_B0034 ) was used as a backbone for a very high level of expression of ComX protein (σx factor).
  • GFP generator, BBa_E0240 (Antiquity Boston University iGEM, 2004): green fluorescent protein reporter ( BBa_E0040 ) with RBS ( BBa_B0032) and double terminator ( BBa_B0015) was used as a reporter for characterization of PcelA and PcomFA promoters.


We Also Built and Characterized the Following Parts:

  • σx Generator, BBa_K1379006: comX gene sequence was assembled with constitutive promoter, RBS and double terminator so ComX protein is produced constitutively.
  • PcelA-GFP Generator, BBa_K1379002: σx-inducible PcelA promoter was assembled with GFP generator.
  • PcomFA-GFP Generator, BBa_K1379003: σx-inducible PcomFA promoter was assembled with GFP generator.
  • σx Generator, PcelA-GFP Generator, BBa_K1379005: σx-generator was assembled with PcelA-GFP generator. Following the assemblage of both constructs, GFP was successfully expressed.
  • σx Generator, PcomFA-GFP Generator, BBa_K1379007: σx-generator was assembled with PcomFA-GFP generator. Following the assemblage of both constructs, GFP was successfully expressed.

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Riboregulator

Human Practice

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