"
Team:Hong Kong HKUST/wetlab/notebook
From 2014.igem.org
(Difference between revisions)
| Line 568: | Line 568: | ||
· Colony PCR of comw-FLAG using Taq DNA Polymerase<br> | · Colony PCR of comw-FLAG using Taq DNA Polymerase<br> | ||
· Inoculation of comX-myc and comW-FLAG<br> | · Inoculation of comX-myc and comW-FLAG<br> | ||
| - | · <br> | + | · Digestion of pCEPcin2 at EcoRI and PstI, EcoRI and SpeI, NcoI and XhoI<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 577: | Line 577: | ||
· Digestion check of comX-myc and comW-FLAG on XbaI and PstI-HF<br> | · Digestion check of comX-myc and comW-FLAG on XbaI and PstI-HF<br> | ||
· Transformation of comX-myc and comW-FLAG<br> | · Transformation of comX-myc and comW-FLAG<br> | ||
| - | · <br> | + | · Digestion of pSB3K3-BBa_J04450 at XbaI and PstI<br> |
| + | · Digestion of pCEPcin2 at EcoRI and PstI<br> | ||
| + | · Inoculation of pSB3K3-BBa_J04450<br> | ||
| + | · PCR of 100bp,150bp and 160bp Pcombox using Vent polymerase<br> | ||
| + | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 588: | Line 592: | ||
· Inoculation of comX-myc and comW-FLAG <br> | · Inoculation of comX-myc and comW-FLAG <br> | ||
· Streaking plates of comX-myc and comW-FLAG<br> | · Streaking plates of comX-myc and comW-FLAG<br> | ||
| - | · <br> | + | · PCR clean-up of 100bp,150bp and 160bp Pcombox<br> |
| - | · <br> | + | · Digestion of pCEPcin2 at EcoRI and PstI<br> |
| - | · <br> | + | · Digestion of pSB3K3-BBa_J04450 at XbaI and PstI<br> |
· <br> | · <br> | ||
<br> <b> July 31</b> <br> | <br> <b> July 31</b> <br> | ||
| Line 598: | Line 602: | ||
· PCR sequencing for comW and comX<br> | · PCR sequencing for comW and comX<br> | ||
· Ethanol precipitation for comW and comX<br> | · Ethanol precipitation for comW and comX<br> | ||
| - | · <br> | + | · Digestion of pSB3K3-BBa_J04450 at XbaI and PstI<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 619: | Line 623: | ||
<div id="satu" align="center"> <h2>August 2014</h2> | <div id="satu" align="center"> <h2>August 2014</h2> | ||
| - | + | <div> | |
| - | <div> | + | |
<a href="#" class="head">Week 1</a> | <a href="#" class="head">Week 1</a> | ||
| + | <div class="content" align="left" style="display: none;"> | ||
| + | <br><b>Aug 1</b> <br> | ||
| + | Wet lab<br> | ||
| + | · Digestion of pSB1C3-BBa_J04450<br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | <div> | ||
| + | <a href="#" class="head">Week 2</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
<br><b>Aug 4</b> <br> | <br><b>Aug 4</b> <br> | ||
| Line 632: | Line 647: | ||
· Sequencing of comX-myc and comW-FLAG<br> | · Sequencing of comX-myc and comW-FLAG<br> | ||
· Inoculation of comX-myc<br> | · Inoculation of comX-myc<br> | ||
| + | · Digestion of 100bp, 150bp, 160bp and 180bp Pcombox at XbaI and PstI<br> | ||
| + | · Ligation of 100bp and 160bp Pcombox with pSB1C3<br> | ||
| + | · Digestion of pCEPcin2 at EcoRI and PstI<br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | |||
<br><b>Aug 5</b> <br> | <br><b>Aug 5</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| Line 642: | Line 664: | ||
· Colony PCR of comX-myc<br> | · Colony PCR of comX-myc<br> | ||
· Inoculation of comX-myc<br> | · Inoculation of comX-myc<br> | ||
| + | · Digestion of pSB1C3-BBa_J04450 at XbaI and PstI<br> | ||
| + | · Ligation of 150bp, 180bp and 249bp Pcombox with pSB1C3<br> | ||
| + | · Transformation of pSB1C3-150bp Pcombox , pSB1C3-180bp Pcombox and pSB1C3-249bp Pcombox<br> | ||
| + | · Gel purification of digested pSB1C3 backbone<br> | ||
| + | · Inoculation of pCEPcin2<br> | ||
· <br> | · <br> | ||
| - | · | + | · <br> |
| - | · | + | · <br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| + | |||
<br><b>Aug 6</b> <br> | <br><b>Aug 6</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| Line 653: | Line 681: | ||
· Sequencing of comW-FLAG using VF2 and VR<br> | · Sequencing of comW-FLAG using VF2 and VR<br> | ||
· Digestion of comX-myc on StyI<br> | · Digestion of comX-myc on StyI<br> | ||
| - | · <br> | + | · Digestion of pCEPcin2 at EcoRI and EcoRV, EcoRI and PstI, EcoRI and SpeI, XhoI and NcoI<br> |
| - | · <br> | + | · Gel purification of digested pSB1C3 backbone<br> |
| + | · Digestion of pSB1C3-BBa_J04450 at XbaI and PstI<br> | ||
| + | · Phosphorylation of 67bp Pcombox oligos<br> | ||
| + | · Oligo annealing for 67bp Pcombox<br> | ||
| + | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| + | |||
<br><b>Aug 7</b> <br> | <br><b>Aug 7</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| Line 664: | Line 697: | ||
· Digestion check of comX on SphI and NcoI<br> | · Digestion check of comX on SphI and NcoI<br> | ||
· Digestion check of comW on XmnI and NcoI<br> | · Digestion check of comW on XmnI and NcoI<br> | ||
| - | · <br> | + | · Digestion of pSB1C3-BBa_J04450 at XbaI and PstI<br> |
| - | · <br> | + | · Colony PCR of 100bp, 150bp, 160bp, 180bp and 249bp Pcombox<br> |
| - | · <br> | + | · Gel purification of digested pSB1C3 backbone<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 680: | Line 713: | ||
· Transformation of comX and comW ligation product<br> | · Transformation of comX and comW ligation product<br> | ||
· Digestion of comX-myc at EcoRI-HF and SpeI-HF<br> | · Digestion of comX-myc at EcoRI-HF and SpeI-HF<br> | ||
| + | · Digestion of pCEPcin2 at EcoRI and EcoRV, XhoI and NcoI<br> | ||
| + | · Ligation of 67bp, 100bp, 150bp, 160bp, 180bp, 249bp and 300bp Pcombox with pSB1C3 backbone<br> | ||
| + | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | |||
</div> | </div> | ||
</div> | </div> | ||
<div> | <div> | ||
| - | <a href="#" class="head">Week | + | <a href="#" class="head">Week 3</a> |
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
<br><b>Aug 11</b> <br> | <br><b>Aug 11</b> <br> | ||
| Line 695: | Line 730: | ||
· Gel purification of comX-myc digested on EcoRI-HF and SpeI<br> | · Gel purification of comX-myc digested on EcoRI-HF and SpeI<br> | ||
· Ligation of comX-myc cut on EcoRI-HF and SpeI into pSB1C3-B0015 cut on EcoRI-HF and XbaI<br> | · Ligation of comX-myc cut on EcoRI-HF and SpeI into pSB1C3-B0015 cut on EcoRI-HF and XbaI<br> | ||
| + | · Restriction check of pSB1C3-Pcombox (100bp, 180bp and 249bp) at XbaI and PstI<br> | ||
| + | · Colony PCR of pSB1C3-Pcombox (67bp, 100bp, 150bp, 160bp, 180bp, 249bp and 300bp)<br> | ||
| + | · Gel purification of digested pSB1C3 backbone<br> | ||
| + | · Digestion of pSB1C3-BBa_J04450 at XbaI and PstI<br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 707: | Line 746: | ||
· Transformation of pSB1C3-B0015.comX-myc ligation product<br> | · Transformation of pSB1C3-B0015.comX-myc ligation product<br> | ||
· Inoculation of pSB1C3-K880005.comX-myc.B0015 and pSB1C3-K880005.comX-myc<br> | · Inoculation of pSB1C3-K880005.comX-myc.B0015 and pSB1C3-K880005.comX-myc<br> | ||
| - | · <br> | + | · Digestion of pCEPcin2 at EcoRI and EcoRV<br> |
| - | · <br> | + | · Restriction check of pSB1C3-Pcombox (100bp, 150bp, 160bp, 180bp and 249bp) at XmnI, NcoI and SpeI<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 714: | Line 753: | ||
Wet lab<br> | Wet lab<br> | ||
· Digestion check of pSB1C3-B0015.comX-myc and pSB1C3-K880005.comX-myc.B0015 on XbaI and PstI-HF<br> | · Digestion check of pSB1C3-B0015.comX-myc and pSB1C3-K880005.comX-myc.B0015 on XbaI and PstI-HF<br> | ||
| - | · <br> | + | · Inoculation of pSB1C3-Pcombox (100bp, 150bp, 160bp, 180bp and 249bp)<br> |
| - | · <br> | + | · Restriction check of pSB1C3-Pcombox (100bp, 150bp, 160bp, 180bp and 249bp) at XmnI, NcoI and SpeI<br> |
| - | · <br> | + | · Colony PCR of pSB1C3-Pcombox (67bp, 100bp, 150bp, 160bp, 180bp and 249bp)<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 731: | Line 770: | ||
· Ligation of comW-FLAG into pSB1C3-B0015 backbone<br> | · Ligation of comW-FLAG into pSB1C3-B0015 backbone<br> | ||
· Re-digestion of comW-FLAG on EcoRI-HF and SpeI-HF<br> | · Re-digestion of comW-FLAG on EcoRI-HF and SpeI-HF<br> | ||
| - | · <br> | + | · Gel check of the digested pSB1C3-Pcombox (100bp, 150bp, 160bp, 180bp and 249bp) obtained on July 13<br> |
| - | · <br> | + | · Colony PCR of Colony PCR of pSB1C3-Pcombox (67bp, 100bp, 150bp, 160bp, 180bp and 249bp)<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 747: | Line 786: | ||
· Ligation of comX-myc into pSB1C3-B0015 backbone<br> | · Ligation of comX-myc into pSB1C3-B0015 backbone<br> | ||
· Inoculation of pSB1C3-K880005.comW-FLAG and pSB1C3-K880005.comX-myc.B0015<br> | · Inoculation of pSB1C3-K880005.comW-FLAG and pSB1C3-K880005.comX-myc.B0015<br> | ||
| - | · <br> | + | · Preparation for submittion of samples to be sequenced<br> |
| - | · <br> | + | · Inoculation of pSB1C3-Pcombox (100bp, 150bp, 160bp, 180bp and 249bp)<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 757: | Line 796: | ||
</div> | </div> | ||
<div> | <div> | ||
| - | <a href="#" class="head">Week | + | <a href="#" class="head">Week 4</a> |
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
<br><b>Aug 18</b> <br> | <br><b>Aug 18</b> <br> | ||
| Line 770: | Line 809: | ||
· Digestion check of pSB1C3-K880005.comX-myc.B0015 on XbaI and PstI-HF<br> | · Digestion check of pSB1C3-K880005.comX-myc.B0015 on XbaI and PstI-HF<br> | ||
· Digestion of pSB1C3-K880005 on SpeI-HF and PstI-HF, digestion clean-up<br> | · Digestion of pSB1C3-K880005 on SpeI-HF and PstI-HF, digestion clean-up<br> | ||
| + | · Sent out pSB1C3-Pcombox (100bp, 150bp and 180bp) samples for commercial sequencing<br> | ||
| + | · Digestion of pSB1C3-Pcombox (100bp, 150bp, 160bp, 180bp and 249bp) at SpeI and PstI<br> | ||
| + | · Gel purification of digested pSB1C3-Pcombox (150bp, 160bp, 180bp and 249bp)<br> | ||
| + | · Inoculation of pSB1C3-BBa_E0240<br> | ||
| + | · <br> | ||
| + | · <br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 778: | Line 823: | ||
· Ligation of pSB1C3-K880005.comX-myc cut on EcoRI-HF and SpeI-HF iinto pSB1C3-B0015 backbone cut on EcoRI-HF and XbaI<br> | · Ligation of pSB1C3-K880005.comX-myc cut on EcoRI-HF and SpeI-HF iinto pSB1C3-B0015 backbone cut on EcoRI-HF and XbaI<br> | ||
· Inoculation of pSB1C3-K880005.comW-FLAG and pSB1C3-K880005.comX-myc.B0015<br> | · Inoculation of pSB1C3-K880005.comW-FLAG and pSB1C3-K880005.comX-myc.B0015<br> | ||
| - | · <br> | + | · Gel purification of digested pSB1C3-Pcombox (100bp)<br> |
| - | · <br> | + | · Ligation of pSB1C3-Pcombox (100bp, 150bp, 160bp, 180bp and 249bp) with BBa_E0240 insert<br> |
| - | · <br> | + | · Transformation of pSB1C3-Pcombox-BBa_E0240 (160bp and 249bp)<br> |
| - | · <br> | + | · Digestion of pSB1C3-BBa_E0240 at XbaI and PstI<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 793: | Line 838: | ||
· Digestion check of pSB1C3-K880005.comX-myc on XbaI and PstI<br> | · Digestion check of pSB1C3-K880005.comX-myc on XbaI and PstI<br> | ||
· Inoculation of pSB1C3-K880005.comX-myc.B0015 and pSB1C3-K880005.comW-FLAG<br> | · Inoculation of pSB1C3-K880005.comX-myc.B0015 and pSB1C3-K880005.comW-FLAG<br> | ||
| - | · <br> | + | · Gel purification of digested pSB1C3-BBa_E0240<br> |
| - | · <br> | + | · Digestion of pSB1C3-180bp Pcombox at SpeI and PstI<br> |
| - | · <br> | + | · Digestion clean-up of pSB1C3-180bp Pcombox<br> |
| - | · <br> | + | · Ligation of pSB1C3-180bp Pcombox with BBa_E0240 insert<br> |
| + | · Transformation of pSB1C3-Pcombox-BBa_E0240 (100bp, 150bp and 180bp)<br> | ||
| + | · Colony PCR for ligated pSB1C3-Pcombox-BBa_E0240 (160bp and 249bp)<br> | ||
| + | · Inoculation of pSB1C3-Pcombox-BBa_E0240 (160bp and 249bp)<br> | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 810: | Line 858: | ||
· Inoculation of pSB1C3-K880005.comW-FLAG<br> | · Inoculation of pSB1C3-K880005.comW-FLAG<br> | ||
· Transformation pSB1C3-K880005.comW-FLAG ligation product and pSB1C3-K880005.comX-myc.B0015<br> | · Transformation pSB1C3-K880005.comW-FLAG ligation product and pSB1C3-K880005.comX-myc.B0015<br> | ||
| + | · Restriction check of pSB1C3-Pcombox-BBa_E0240 (160bp and 249bp) at NcoI<br> | ||
| + | · Colony PCR for ligated pSB1C3-Pcombox-BBa_E0240 (100bp, 150bp and 180bp)<br> | ||
| + | · Inoculation of pSB1C3-Pcombox-BBa_E0240 (100bp, 150bp and 180bp)<br> | ||
| + | · Received sequencing result of pSB1C3-Pcombox (100bp, 150bp and 180bp)<br> | ||
· <br> | · <br> | ||
| - | · | + | · <br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 819: | Line 871: | ||
· Digestion of pSB1C3-K880005.comX-myc.B0015 on EcoRI-HF and SpeI-HF<br> | · Digestion of pSB1C3-K880005.comX-myc.B0015 on EcoRI-HF and SpeI-HF<br> | ||
· Inoculation pSB1C3-K880005.comX-myc.B0015<br> | · Inoculation pSB1C3-K880005.comX-myc.B0015<br> | ||
| - | · <br> | + | · Restriction check of pSB1C3-Pcombox-BBa_E0240 (100bp, 150bp and 180bp) at NcoI and XmnI<br> |
| - | · <br> | + | · PCR of pSB1C3-Pcombox-BBa_E0240 (160bp and 249bp) using Vent polymerase <br> |
| - | · <br> | + | · Sent out pSB1C3-Pcombox-BBa_E0240 (160bp and 249bp) for commercial sequencing<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 834: | Line 886: | ||
</div> | </div> | ||
<div> | <div> | ||
| - | <a href="#" class="head">Week | + | <a href="#" class="head">Week 5</a> |
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
<br><b>Aug 25</b> <br> | <br><b>Aug 25</b> <br> | ||
| Line 843: | Line 895: | ||
Digestion of pSB1C3-K880005.comX-myc.B0015 on SpeI-HF and PstI-HF, gel extraction and gel purification | Digestion of pSB1C3-K880005.comX-myc.B0015 on SpeI-HF and PstI-HF, gel extraction and gel purification | ||
Inoculation of pSB1C3-K880005.comX-myc.B0015 for glycerol stock<br> | Inoculation of pSB1C3-K880005.comX-myc.B0015 for glycerol stock<br> | ||
| - | · <br> | + | · Received sequencing result of pSB1C3-Pcombox (160bp and 249bp)<br> |
| - | · <br> | + | · Restriction check of pSB1C3-Pcombox-BBa_E0240 (100bp, 150bp and 180bp) at NcoI and XmnI<br> |
| - | · <br> | + | · Digestion of pSB1C3-Pcombox (100bp, 150bp and 160bp, 180bp and 249bp) at XbaI and PstI<br> |
| - | · <br> | + | · Gel purification of digested Pcombox-BBa_E0240 (100bp, 150bp and 180bp) insert<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 859: | Line 911: | ||
· Inoculation of pSB1C3-K880005.comW-FLAG.B0015<br> | · Inoculation of pSB1C3-K880005.comW-FLAG.B0015<br> | ||
· Transformation of pSB1C3-K880005.comW-FLAG<br> | · Transformation of pSB1C3-K880005.comW-FLAG<br> | ||
| - | · <br> | + | · Ligation of comX, 100bp Pcombox-BBa_E0240 and pSB3K3 backbone<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 871: | Line 923: | ||
· Transformation of pSB1C3-K880005.comW-FLAG<br> | · Transformation of pSB1C3-K880005.comW-FLAG<br> | ||
· Inoculation of pSB1C3-K880005.comW-FLAG.B0015 and pSB1C3-K880005.comW-FLAG<br> | · Inoculation of pSB1C3-K880005.comW-FLAG.B0015 and pSB1C3-K880005.comW-FLAG<br> | ||
| - | · <br> | + | · Colony PCR of pSB1C3-Pcombox-BBa_E0240 (180bp and 249bp)<br> |
| - | · <br> | + | · Ligation of comX, 100bp Pcombox-BBa_E0240 and pSB3K3 backbone<br> |
| - | · <br> | + | · Inoculation of pSB1C3-Pcombox-BBa_E0240 (100bp, 180bp and 249bp), pSB1C3-Pcombox (160bp)<br> |
· <br> | · <br> | ||
<br><b>Aug 28</b> <br> | <br><b>Aug 28</b> <br> | ||
| Line 882: | Line 934: | ||
· Inoculation of pSB1C3-K880005.comW-FLAG.B0015 and pSB1C3-K880005.comW-FLAG<br> | · Inoculation of pSB1C3-K880005.comW-FLAG.B0015 and pSB1C3-K880005.comW-FLAG<br> | ||
· Ligation of pSB1C3-K880005.comW-FLAG into pSB1C3-B0015 backbone<br> | · Ligation of pSB1C3-K880005.comW-FLAG into pSB1C3-B0015 backbone<br> | ||
| - | · <br> | + | · Restriction check of pSB1C3-Pcombox-BBa_E0240 (180bp and 249bp)<br> |
| - | · <br> | + | · Colony PCR of pSB3K3-comX-100bp Pcombox-BBa_E0240<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 891: | Line 943: | ||
· Digestion check of pSB1C3-K880005.comW-FLAG.B0015 on XbaI and PstI-HF<br> | · Digestion check of pSB1C3-K880005.comW-FLAG.B0015 on XbaI and PstI-HF<br> | ||
· Digestion check of pSB1C3-K880005.comW-FLAG on EcoRI-HF and SpeI-HF<br> | · Digestion check of pSB1C3-K880005.comW-FLAG on EcoRI-HF and SpeI-HF<br> | ||
| - | · <br> | + | · Sent out pSB1C3-160bp Pcombox for commercial sequencing<br> |
| - | · <br> | + | · Digestion of pSB1C3-160bp Pcombox at SpeI and PstI<br> |
| - | · <br> | + | · Colony PCR for pSB3K3-comX-100bp Pcombox-BBa_E0240<br> |
| - | · <br> | + | · Ligation of pSB1C3-160bp Pcombox with BBa_E0240 insert<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 903: | Line 955: | ||
</div> | </div> | ||
</div> | </div> | ||
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</div> | </div> | ||
| Line 960: | Line 982: | ||
· Digestion check of pSB1C3-K880005.comX-myc.B0015.Combox.GFP on XmnI<br> | · Digestion check of pSB1C3-K880005.comX-myc.B0015.Combox.GFP on XmnI<br> | ||
· Inoculation of pSB1C3-K880005.comX-myc.B0015.Combox.GFP<br> | · Inoculation of pSB1C3-K880005.comX-myc.B0015.Combox.GFP<br> | ||
| - | · <br> | + | · Received the sequencing result of pSB1C3-160bp Pcombox<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 972: | Line 994: | ||
· Ligation of pSB1C3-K880005.comX-myc.B0015.Combox.GFP cut on EcoRI-HF and PstI-HF into pSB3K3 cut on EcoRI-HF and PstI-HF<br> | · Ligation of pSB1C3-K880005.comX-myc.B0015.Combox.GFP cut on EcoRI-HF and PstI-HF into pSB3K3 cut on EcoRI-HF and PstI-HF<br> | ||
· Re-digestion of pSB1C3-K880005.comX-myc.B0015.Combox.GFP on EcoRI-HF and PstI-HF, gel extraction and gel purification<br> | · Re-digestion of pSB1C3-K880005.comX-myc.B0015.Combox.GFP on EcoRI-HF and PstI-HF, gel extraction and gel purification<br> | ||
| - | · <br> | + | · Restriction check of pSB1C3-160bp Pcombox-BBa_E0240 at XmnI and NcoI<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 985: | Line 1,007: | ||
· Streak plate for pSB1C3-K880005.comX-myc.B0015.Combox.GFP<br> | · Streak plate for pSB1C3-K880005.comX-myc.B0015.Combox.GFP<br> | ||
· Inoculation of pSB1C3-K880005.comW-FLAG.B0015<br> | · Inoculation of pSB1C3-K880005.comW-FLAG.B0015<br> | ||
| - | · <br> | + | · Restriction check of pSB1C3-160bp Pcombox-BBa_E0240 at NcoI and NotI<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 1,000: | Line 1,022: | ||
· Ligation of Combox + GFP into pSB3K3 backbone cut on EcoRI-HF and PstI-HF<br> | · Ligation of Combox + GFP into pSB3K3 backbone cut on EcoRI-HF and PstI-HF<br> | ||
· Transformation of pSB3K3-Combox.GFP ligation product<br> | · Transformation of pSB3K3-Combox.GFP ligation product<br> | ||
| - | · <br> | + | · Digestion of pSB1C3-160bp Pcombox-BBa_E0240 at XbaI and PstI<br> |
| - | · <br> | + | · Ligation of 160bp Pcombox-BBa_E0240,comX and pSB3K3<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 1,068: | Line 1,090: | ||
<br><b>Sept 11</b> <br> | <br><b>Sept 11</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| - | · <br> | + | · Restriction check of pSB3K3-comX-160bp Pcombox-BBa_E0240 at EcoRI and PstI<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 1,081: | Line 1,103: | ||
<br><b>Sept 12</b> <br> | <br><b>Sept 12</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| - | · <br> | + | · Restriction check of pSB3K3-160bp Pcombox-BBa_E0240 at EcoRI and PstI<br> |
| - | · <br> | + | · Transformation of pSB3K3-160bp Pcombox-BBa_E0240<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 1,130: | Line 1,152: | ||
· Digestion check of pSB1C3-K880005.comX-myc.B0015.Combox on XmnI<br> | · Digestion check of pSB1C3-K880005.comX-myc.B0015.Combox on XmnI<br> | ||
· Digestion check of Combox + GFP on XmnI and PstI<br> | · Digestion check of Combox + GFP on XmnI and PstI<br> | ||
| - | · <br> | + | · Restriction check of pSB3K3-160bp Pcombox-BBa_E0240 at NotI and PstI<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 1,172: | Line 1,194: | ||
· Digestion check of pSB1C3-K880005.comX-myc.B0015 on XbaI and PstI<br> | · Digestion check of pSB1C3-K880005.comX-myc.B0015 on XbaI and PstI<br> | ||
· Inoculation of pSB1C3-K880005.comW-FLAG.B0015<br> | · Inoculation of pSB1C3-K880005.comW-FLAG.B0015<br> | ||
| - | · <br> | + | · Ligation of pSB3K3 backbone with 160bp Pcombox-BBa_E0240<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 1,185: | Line 1,207: | ||
· Digestion of pSB1C3-K880005.comX-myc.B0015 and pSB1C3-K880005.comX on EcoRI-HF and SpeI-HF, gel extraction and gel purification<br> | · Digestion of pSB1C3-K880005.comX-myc.B0015 and pSB1C3-K880005.comX on EcoRI-HF and SpeI-HF, gel extraction and gel purification<br> | ||
· Ligation of pSB1C3-K880005.comX into pSB1C3-B0015 cut on EcoRI-HF and XbaI<br> | · Ligation of pSB1C3-K880005.comX into pSB1C3-B0015 cut on EcoRI-HF and XbaI<br> | ||
| - | · <br> | + | · Transformation of pSB3K3-160bp Pcombox-BBa_E0240 ligated on Sept 22<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 1,196: | Line 1,218: | ||
Wet lab<br> | Wet lab<br> | ||
· Digestion check of pSB1C3-K880005.comX.B0015 on XbaI and PstI, gel extraction and gel purification<br> | · Digestion check of pSB1C3-K880005.comX.B0015 on XbaI and PstI, gel extraction and gel purification<br> | ||
| - | · <br> | + | · Inoculation of bacterial colonies carrying pSB3K3-160bp Pcombox-BBa_E0240<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 1,208: | Line 1,230: | ||
<br><b>Sept 25</b> <br> | <br><b>Sept 25</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| - | · <br> | + | · Restrcition digestion of ligated pSB3K3-160Pcombox-BBa_E0240<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 1,221: | Line 1,243: | ||
<br><b>Sept 26</b> <br> | <br><b>Sept 26</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
| - | · <br> | + | · Transformation of pSB3K3-160Pcombox-BBa_E0240 sample1<br> |
| - | · <br> | + | · Streak plate for pSB3K3-BBa_I20260 and pSB3K3-BBa_E0240<br> |
· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 1,255: | Line 1,277: | ||
Wet lab<br> | Wet lab<br> | ||
· Digestion of RBS+comX-myc+B0015 on XbaI and PstI<br> | · Digestion of RBS+comX-myc+B0015 on XbaI and PstI<br> | ||
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· <br> | · <br> | ||
· <br> | · <br> | ||
| Line 1,375: | Line 1,358: | ||
<!-- JJJJJJJJJJJJ UUUUUUUUUUUU N N EEEEEEEEEEE 2 0 1 3----------------> | <!-- JJJJJJJJJJJJ UUUUUUUUUUUU N N EEEEEEEEEEE 2 0 1 3----------------> | ||
| - | <div id="satu" align="center"> <h2>June | + | <div id="satu" align="center"> <h2>June 2014</h2> |
<div> | <div> | ||
<a href="#" class="head">Week 1</a> | <a href="#" class="head">Week 1</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br><b>June | + | <br><b>June 2</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,386: | Line 1,369: | ||
· <br> | · <br> | ||
· <br><br> | · <br><br> | ||
| - | <br><b>June | + | <br><b>June 3</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,392: | Line 1,375: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>June | + | <br><b>June 4</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,398: | Line 1,381: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>June | + | <br><b>June 5</b> <br> |
| + | Wet lab<br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | <br><b>June 6</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,404: | Line 1,393: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | |||
</div> | </div> | ||
| Line 1,411: | Line 1,399: | ||
<a href="#" class="head">Week 2</a> | <a href="#" class="head">Week 2</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br><b>June | + | <br><b>June 9</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,417: | Line 1,405: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>June | + | <br><b>June 10</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,423: | Line 1,411: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>June | + | <br><b>June 11</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,429: | Line 1,417: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>June | + | <br><b>June 12</b> <br> |
| + | Wet lab<br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | <br><b>June 13</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,440: | Line 1,434: | ||
<a href="#" class="head">Week 3</a> | <a href="#" class="head">Week 3</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br><b>June | + | <br><b>June 16</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,446: | Line 1,440: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>June | + | <br><b>June 17</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,452: | Line 1,446: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>June | + | <br><b>June 18</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,458: | Line 1,452: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>June | + | <br><b>June 19</b> <br> |
| + | Wet lab<br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | <br><b>June 20</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,464: | Line 1,464: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | |||
</div> | </div> | ||
| Line 1,471: | Line 1,470: | ||
<a href="#" class="head">Week 4</a> | <a href="#" class="head">Week 4</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br><b>June | + | <br><b>June 23</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,477: | Line 1,476: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>June | + | <br><b>June 24</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,483: | Line 1,482: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>June | + | <br><b>June 25</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,489: | Line 1,488: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>June | + | <br><b>June 26</b> <br> |
| + | Wet lab<br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | <br><b>June 27</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,495: | Line 1,500: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | |||
</div> | </div> | ||
| Line 1,502: | Line 1,506: | ||
<a href="#" class="head">Week 5</a> | <a href="#" class="head">Week 5</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br><b>June | + | <br><b>June 30</b> <br> |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,526: | Line 1,512: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| + | |||
</div> | </div> | ||
| Line 1,546: | Line 1,533: | ||
| - | <div id="satu" align="center"> <h2><center>July | + | <div id="satu" align="center"> <h2><center>July 2014</center></h2> |
<div> | <div> | ||
<a href="#" class="head">Week 1</a> | <a href="#" class="head">Week 1</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br> <b> July | + | <br> <b> July 1 </b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,558: | Line 1,545: | ||
· <br> | · <br> | ||
| - | <br> <b> July | + | <br> <b> July 2 </b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,565: | Line 1,552: | ||
· <br> | · <br> | ||
| - | <br> <b> July | + | <br> <b> July 3</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,571: | Line 1,558: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br> <b> July | + | <br> <b> July 4</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,585: | Line 1,572: | ||
<a href="#" class="head">Week 2</a> | <a href="#" class="head">Week 2</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br> <b> July | + | <br> <b> July 7 </b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,592: | Line 1,579: | ||
· <br> | · <br> | ||
| - | <br> <b> July | + | <br> <b> July 8 </b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,599: | Line 1,586: | ||
· <br> | · <br> | ||
| - | <br> <b> July | + | <br> <b> July 9</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,605: | Line 1,592: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br> <b> July | + | <br> <b> July 10</b> <br> |
| + | Wet lab<br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | <br> <b> July 11</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,617: | Line 1,610: | ||
<a href="#" class="head">Week 3</a> | <a href="#" class="head">Week 3</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br> <b> July | + | <br> <b> July 14 </b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,624: | Line 1,617: | ||
· <br> | · <br> | ||
| - | <br> <b> July | + | <br> <b> July 15 </b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,631: | Line 1,624: | ||
· <br> | · <br> | ||
| - | <br> <b> July | + | <br> <b> July 16</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,637: | Line 1,630: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br> <b> July | + | <br> <b> July 17</b> <br> |
| + | Wet lab<br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | <br> <b> July 18</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,643: | Line 1,642: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | |||
</div> | </div> | ||
</div> | </div> | ||
| Line 1,650: | Line 1,648: | ||
<a href="#" class="head">Week 4</a> | <a href="#" class="head">Week 4</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br> <b> July | + | <br> <b> July 21 </b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,657: | Line 1,655: | ||
· <br> | · <br> | ||
| - | <br> <b> July | + | <br> <b> July 22 </b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,664: | Line 1,662: | ||
· <br> | · <br> | ||
| - | <br> <b> July | + | <br> <b> July 23</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,670: | Line 1,668: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br> <b> July | + | <br> <b> July 24</b> <br> |
| + | Wet lab<br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | <br> <b> July 25</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,682: | Line 1,686: | ||
<a href="#" class="head">Week 5</a> | <a href="#" class="head">Week 5</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br> <b> July | + | <br> <b> July 28 </b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,689: | Line 1,693: | ||
· <br> | · <br> | ||
| - | <br> <b> July | + | <br> <b> July 29 </b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,696: | Line 1,700: | ||
· <br> | · <br> | ||
| - | <br> <b> July | + | <br> <b> July 30</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,702: | Line 1,706: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br> <b> July | + | <br> <b> July 31</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,725: | Line 1,729: | ||
| - | <div id="satu" align="center"> <h2>August | + | <div id="satu" align="center"> <h2>August 2014</h2> |
<div> | <div> | ||
<a href="#" class="head">Week 1</a> | <a href="#" class="head">Week 1</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br><b>Aug | + | <br><b>Aug 1</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,736: | Line 1,740: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | |||
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</div> | </div> | ||
| Line 1,761: | Line 1,746: | ||
<a href="#" class="head">Week 2</a> | <a href="#" class="head">Week 2</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br><b>Aug | + | <br><b>Aug 4</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,767: | Line 1,752: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>Aug | + | <br><b>Aug 5</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,773: | Line 1,758: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>Aug | + | <br><b>Aug 6</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,779: | Line 1,764: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>Aug | + | <br><b>Aug 7</b> <br> |
| + | Wet lab<br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | <br><b>Aug 8</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,785: | Line 1,776: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | |||
</div> | </div> | ||
| Line 1,792: | Line 1,782: | ||
<a href="#" class="head">Week 3</a> | <a href="#" class="head">Week 3</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br><b>Aug | + | <br><b>Aug 11</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,798: | Line 1,788: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>Aug | + | <br><b>Aug 12</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,804: | Line 1,794: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>Aug | + | <br><b>Aug 13</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,810: | Line 1,800: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>Aug | + | <br><b>Aug 14</b> <br> |
| + | Wet lab<br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | <br><b>Aug 15</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,816: | Line 1,812: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | |||
| Line 1,824: | Line 1,819: | ||
<a href="#" class="head">Week 4</a> | <a href="#" class="head">Week 4</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br><b>Aug | + | <br><b>Aug 18</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,830: | Line 1,825: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>Aug | + | <br><b>Aug 19</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,836: | Line 1,831: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>Aug | + | <br><b>Aug 20</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,842: | Line 1,837: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>Aug | + | <br><b>Aug 21</b> <br> |
| + | Wet lab<br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | <br><b>Aug 22</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,848: | Line 1,849: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | |||
</div> | </div> | ||
| Line 1,855: | Line 1,855: | ||
<a href="#" class="head">Week 5</a> | <a href="#" class="head">Week 5</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br><b>Aug | + | <br><b>Aug 25</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,861: | Line 1,861: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>Aug | + | <br><b>Aug 26</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,867: | Line 1,867: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>Aug | + | <br><b>Aug 27</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,873: | Line 1,873: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | <br><b>Aug | + | <br><b>Aug 28</b> <br> |
| + | Wet lab<br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | · <br> | ||
| + | <br><b>Aug 29</b> <br> | ||
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,879: | Line 1,885: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
| - | |||
| - | |||
</div> | </div> | ||
</div> | </div> | ||
| Line 1,899: | Line 1,903: | ||
<!---- SSSSSSSS EEEEEEEE P T EEEEEEEE M M BBBBBBBB EEEEEEEEE R R 2 0 1 3--------> | <!---- SSSSSSSS EEEEEEEE P T EEEEEEEE M M BBBBBBBB EEEEEEEEE R R 2 0 1 3--------> | ||
| - | <div id="satu" align="center"> <h2>September | + | <div id="satu" align="center"> <h2>September 2014</h2> |
<div> | <div> | ||
<a href="#" class="head">Week 1</a> | <a href="#" class="head">Week 1</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br><b>Sept | + | <br><b>Sept 1</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,911: | Line 1,915: | ||
· <br> | · <br> | ||
| - | <br><b>Sept | + | <br><b>Sept 2</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,918: | Line 1,922: | ||
· <br> | · <br> | ||
| - | <br><b>Sept | + | <br><b>Sept 3</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,925: | Line 1,929: | ||
· <br> | · <br> | ||
| - | <br><b>Sept | + | <br><b>Sept 4</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,932: | Line 1,936: | ||
· <br> | · <br> | ||
| - | <br><b>Sept | + | <br><b>Sept 5</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,945: | Line 1,949: | ||
<a href="#" class="head">Week 2</a> | <a href="#" class="head">Week 2</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br><b>Sept | + | <br><b>Sept 8</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,953: | Line 1,957: | ||
| - | <br><b>Sept | + | <br><b>Sept 9</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,960: | Line 1,964: | ||
· <br> | · <br> | ||
| - | <br><b>Sept | + | <br><b>Sept 10</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,967: | Line 1,971: | ||
· <br> | · <br> | ||
| - | <br><b>Sept | + | <br><b>Sept 11</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,974: | Line 1,978: | ||
· <br> | · <br> | ||
| - | <br><b>Sept | + | <br><b>Sept 12</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,985: | Line 1,989: | ||
<a href="#" class="head">Week 3</a> | <a href="#" class="head">Week 3</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br><b>Sept | + | <br><b>Sept 15</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 1,993: | Line 1,997: | ||
| - | <br><b>Sept | + | <br><b>Sept 16</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 2,000: | Line 2,004: | ||
· <br> | · <br> | ||
| - | <br><b>Sept | + | <br><b>Sept 17</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 2,007: | Line 2,011: | ||
· <br> | · <br> | ||
| - | <br><b>Sept | + | <br><b>Sept 18</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 2,014: | Line 2,018: | ||
· <br> | · <br> | ||
| - | <br><b>Sept | + | <br><b>Sept 19</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 2,027: | Line 2,031: | ||
<a href="#" class="head">Week 4</a> | <a href="#" class="head">Week 4</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br><b>Sept | + | <br><b>Sept 22</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 2,034: | Line 2,038: | ||
· <br> | · <br> | ||
| - | <br><b>Sept | + | <br><b>Sept 23</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 2,041: | Line 2,045: | ||
· <br> | · <br> | ||
| - | <br><b>Sept | + | <br><b>Sept 24</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 2,048: | Line 2,052: | ||
· <br> | · <br> | ||
| - | <br><b>Sept | + | <br><b>Sept 25</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 2,055: | Line 2,059: | ||
· <br> | · <br> | ||
| - | <br><b>Sept | + | <br><b>Sept 26</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 2,068: | Line 2,072: | ||
<a href="#" class="head">Week 5</a> | <a href="#" class="head">Week 5</a> | ||
<div class="content" align="left" style="display: none;"> | <div class="content" align="left" style="display: none;"> | ||
| - | <br><b>Sept | + | <br><b>Sept 29</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 2,076: | Line 2,080: | ||
| - | <br><b>Sept | + | <br><b>Sept 30</b> <br> |
Wet lab<br> | Wet lab<br> | ||
· <br> | · <br> | ||
| Line 2,082: | Line 2,086: | ||
· <br> | · <br> | ||
· <br> | · <br> | ||
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</div> | </div> | ||
Revision as of 12:47, 10 October 2014
Notebook
|
Throughout the course of our project, we have continuously recorded our daily work, such as wetlab activities and research findings. The notebook for Pneuumonsensor and Riboregulator are displayed separately. PneumosensorRiboregulator |
Home |
Pneumosensor |
Riboregulator |
Human Practice |
Team |
WetLab |
Achievement |
