Team:Bielefeld-CeBiTec/Results/CO2-fixation

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The second module aims to change E.coli in a way that it binds carbon dioxide like an autotroph organism. For this we want to establish the Calvin-cycle. E.coli has all enzymes for the Calvin-cycle except of three.<br>
The second module aims to change E.coli in a way that it binds carbon dioxide like an autotroph organism. For this we want to establish the Calvin-cycle. E.coli has all enzymes for the Calvin-cycle except of three.<br>
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      <a href="https://static.igem.org/mediawiki/2014/5/54/Bielefeld-CeBiTec_2014-10-11_Carboxy_weiss_wiki.png" target="_blank"><img src="https://static.igem.org/mediawiki/2014/5/54/Bielefeld-CeBiTec_2014-10-11_Carboxy_weiss_wiki.png" width="450px"></a><br>
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<font size="1" style="text-align:center;">Missing enzymes in Calvin-cycle</font>
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The different results for all three enzymes are mentioned in the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/Calvin-Cycle" target="_blank">Calvin-cycle</a> section. One important step for the carbon dioxide fixation is the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/RuBisCO" target="_blank">RuBisCO</a> (Ribulose 1,5-bisphosphate carboxylase/oxygenase). For E.coli we decided to transform genes for the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/Carboxysome" target="_blank">carboxysome</a> because of its properties.  
The different results for all three enzymes are mentioned in the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/Calvin-Cycle" target="_blank">Calvin-cycle</a> section. One important step for the carbon dioxide fixation is the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/RuBisCO" target="_blank">RuBisCO</a> (Ribulose 1,5-bisphosphate carboxylase/oxygenase). For E.coli we decided to transform genes for the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/Carboxysome" target="_blank">carboxysome</a> because of its properties.  
      
      

Revision as of 19:31, 11 October 2014


CO2 fixation

The second module aims to change E.coli in a way that it binds carbon dioxide like an autotroph organism. For this we want to establish the Calvin-cycle. E.coli has all enzymes for the Calvin-cycle except of three.


Missing enzymes in Calvin-cycle
The different results for all three enzymes are mentioned in the Calvin-cycle section. One important step for the carbon dioxide fixation is the RuBisCO (Ribulose 1,5-bisphosphate carboxylase/oxygenase). For E.coli we decided to transform genes for the carboxysome because of its properties.

Here you will find information about the execution of our experiments.

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