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Team:Bielefeld-CeBiTec/Notebook/Journal/CO2-fixation/Oct
From 2014.igem.org
(Difference between revisions)
| Line 52: | Line 52: | ||
<li><b>tkt</b></li> | <li><b>tkt</b></li> | ||
<ul> | <ul> | ||
| - | <li>This week we | + | <li>This week we wanted to do the enzyme assay for the SBPase.</li> |
<ul> | <ul> | ||
<li>The purified enzyme from His-Tag purification werde <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#ProteinBufferExchangeAndDesalting" target="_blank">concentrated and desalted</a> with <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#ProteinPurification" target="_blank">Amicon Ultra-15</a> with 10kD cut-off</li> | <li>The purified enzyme from His-Tag purification werde <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#ProteinBufferExchangeAndDesalting" target="_blank">concentrated and desalted</a> with <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#ProteinPurification" target="_blank">Amicon Ultra-15</a> with 10kD cut-off</li> | ||
| Line 61: | Line 61: | ||
<li><b>fba</b></li> | <li><b>fba</b></li> | ||
<ul> | <ul> | ||
| - | <li>This week we | + | <li>This week we wanted to do the enzyme assay for the SBPase.</li> |
<ul> | <ul> | ||
<li>The purified enzyme from His-Tag purification werde <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#ProteinBufferExchangeAndDesalting" target="_blank">concentrated and desalted</a> with <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#ProteinPurification" target="_blank">Amicon Ultra-15</a> with 10kD cut-off</li> | <li>The purified enzyme from His-Tag purification werde <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#ProteinBufferExchangeAndDesalting" target="_blank">concentrated and desalted</a> with <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#ProteinPurification" target="_blank">Amicon Ultra-15</a> with 10kD cut-off</li> | ||
| Line 70: | Line 70: | ||
<li><b>glpX</b></li> | <li><b>glpX</b></li> | ||
<ul> | <ul> | ||
| - | <li>This week we | + | <li>This week we wanted to do the enzyme assay for the SBPase.</li> |
<ul> | <ul> | ||
<li>The purified enzyme from His-Tag purification werde <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#ProteinBufferExchangeAndDesalting" target="_blank">concentrated and desalted</a> with <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#ProteinPurification" target="_blank">Amicon Ultra-15</a> with 10kD cut-off</li> | <li>The purified enzyme from His-Tag purification werde <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#ProteinBufferExchangeAndDesalting" target="_blank">concentrated and desalted</a> with <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#ProteinPurification" target="_blank">Amicon Ultra-15</a> with 10kD cut-off</li> | ||
Revision as of 22:50, 13 October 2014
 |
October |
- tkt
- This week we wanted to do the enzyme assay for the SBPase.
- The purified enzyme from His-Tag purification werde concentrated and desalted with Amicon Ultra-15 with 10kD cut-off
- fba
- This week we wanted to do the enzyme assay for the SBPase.
- The purified enzyme from His-Tag purification werde concentrated and desalted with Amicon Ultra-15 with 10kD cut-off
- glpX
- This week we wanted to do the enzyme assay for the SBPase.
- The purified enzyme from His-Tag purification werde concentrated and desalted with Amicon Ultra-15 with 10kD cut-off
