Team:Aachen/Project/2D Biosensor

From 2014.igem.org

(Difference between revisions)
(→2D Biosensor)
(→2D Biosensor)
Line 4: Line 4:
= 2D Biosensor =
= 2D Biosensor =
-
''Cellock Holmes'' is devised based upon a SynBio approach comprising of a '''two-dimensional biosensor and a measurement device'''. The two-dimensional biosensor (Figure 1) is designed to recognize quorum sensing molecules secreted by the pathogen cells and generate a distinct fluorescence signal.
+
''Cellock Holmes'' is devised based upon a SynBio approach comprising of a '''two-dimensional biosensor and a measurement device'''. The two-dimensional biosensor is designed to recognize quorum sensing molecules secreted by the pathogen cells and generate a distinct fluorescence signal, while the measurement device is designed to recognize and analyse the produced signal.
-
<html><figure style="float: left; margin: 0px 15px 15px 0px;">
+
For our biosensor, our team genetically modified ''E. coli'' cells to be able to elecit a fluorescent response to quorum sensing molecules produced by the pathogen ''Pseudomonas aeruginosa''.
-
<img src="https://static.igem.org/mediawiki/2014/thumb/e/ec/Aachen_Infografik_sampling_biosensor.png/700px-Aachen_Infografik_sampling_biosensor.png" width="700" />
+
-
<figcaption>
+
-
<i>Figure 1: Sampling of microorganisms (left) for the detection with a 2D biosensor (right)</i></figcaption>
+
-
</figure></html>
+
-
 
+
-
In parallel, the team also aims to develop a more flexible novel molecular detection system for the biosensor based on binding proteins and genetic probes.
+
-
 
+
-
These molecular approaches were selected as a reasonable spectrum from the established to novel and high-potential modules.
+
-
 
+
-
The Aachen team is committed to consider all aspects of the entire project, including biosafety. The living cells inside the measurement device are ensured to be killed by irradiation with strong UV light. In this way neither the sampled pathogens nor the genetically modified sensor cells can escape our biosensor unit.
+
{{Team:Aachen/Footer}}
{{Team:Aachen/Footer}}

Revision as of 14:35, 6 October 2014

2D Biosensor

Cellock Holmes is devised based upon a SynBio approach comprising of a two-dimensional biosensor and a measurement device. The two-dimensional biosensor is designed to recognize quorum sensing molecules secreted by the pathogen cells and generate a distinct fluorescence signal, while the measurement device is designed to recognize and analyse the produced signal.

For our biosensor, our team genetically modified E. coli cells to be able to elecit a fluorescent response to quorum sensing molecules produced by the pathogen Pseudomonas aeruginosa.


Contact Disclaimer

Retrieved from "http://2014.igem.org/Team:Aachen/Project/2D_Biosensor"