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Revision as of 13:48, 5 October 2014

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Organisms

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Organisms

Escherichia coli

Scanning electron micrograph of Escherichia coli, grown in culture and adhered to a cover slip.

The strain KRX is used for all approaches. Coding sequences of this organism were used for different purposes:

frd - Fumarate reductase
Cytochromes

Domain	Bacteria
Phylum	Proteobacteria
Class	Gammaproteobacteria
Order	Enterobacteriales
Family	Enterobacteriaceae
Genus	Escherichia
Species	E. coli

Lactococcus lactis

Scanning Electron Micrograph of Enterococcus faecalis

Coding sequences of this organism were used for the isobutanol production:

kivD - α-ketoisovalerate decarboxylase
adhA - Alcoholdehydrogenase

Domain	Bacteria
Phylum	Firmicutes
Class	Bacilli
Order	Lactobacillales
Family	Streptococcaceae
Genus	Lactococcus
Species	L. lactis

Halothiobacillus neapolitanus

(A) Electron micrograph of Halothiobacillus neapolitanus cells, arrows highlight carboxysomes. (B) Image of intact carboxysomes isolated from H. neapolitanus. Scale bars indicate 100 nm.

Coding sequences of this organism were used for the carboxysome:

sap - shell associated protein
can - carbonic anhydrase
csoS1-4 - shell protein
csoS1D - pore protein

Domain	Bacteria
Phylum	Proteobacteria
Class	γ-Proteobacteria
Order	Chromatiales
Family	Halothiobacillaceae
Genus	Halothiobacillus
Species	H. neapolitanus

Bacillus subtilis

The Bacterium Bacillus subtilis taken with a Tecnai T-12 TEM. Taken by Allon Weiner, The Weizmann Institute of Science, Rehovot, Israel. 2006.

A coding sequence of this organism was used for the isobutanol production:

alsS - α-acetolactate synthase

Domain	Bacteria
Phylum	Firmicutes
Class	Bacilli
Order	Bacillales
Family	Bacillaceae
Genus	Bacillus
Species	B. subtilis

Synechococcus elongatus

Synechococcus PCC 7002 in DIC microscopy. Imaging was performed with the Olympus BX61 microscope and a UPlanSApo 100× NA 1.40 oil immersion objective (Olympus). Pictures were acquired at room temperature in water with a camera (SPOT; Diagnostic Instruments, Inc.) using MetaMorph software (MDS Analytical Technologies).

A coding sequence of this organism was used for the carbon dioxide fixation:

RuBisCO - Ribulose 1,5-bisphosphate carboxylase / oxygenase
prkA - Phosphoribulokinase

Domain	Bacteria
Phylum	Cyanobacteria
Class	Oscillatoriophycideae
Order	Chroococcales
Genus	Synechococcus
Species	S. elongatus

Actinobacillus succinogenes

Actinobacillus lignieresi. Gram stain.

A coding sequence of this organism was used for the transport of reduction equivalents:

Asuc_1813/1814/1815/1816 - fumarate reductase

Domain	Bacteria
Phylum	Proteobacteria
Class	γ-Proteobacteria
Order	Pasteurellales
Family	Pasteurellaceae
Genus	Actinobacillus
Species	A. succinogenes

Geobacter sulfurreducens

A coding sequence of this organism was used for the transport of electrons:

Cytochromes

Domain	Bacteria
Phylum	Proteobacteria
Subphylum	δ / ε-Proteobacteria
Class	δ-Proteobacteria
Order	Desulfuromonadales
Family	Geobacteraceae
Genus	Geobacter
Species	G. sulfurreducens

Corynebacterium glutamicum

Phase contrast of Corynebacterium glutamicum

A coding sequence of this organism was used for an enzyme assay:

pgk - Phosphoglycerate kinase

Domain	Bacteria
Phylum	Actinobacteria
Class	Actinobacteridae
Order	Actinomycetales
Suborder	Corynebacterineae
Family	Corynebacteriaceae
Genus	Corynebacterium
Species	C. glutamicum

Bacillus methanolicus

Microscopic image of Bacillus subtilis (ATCC 6633). Gram staining, magnification:1,000. The oval unstained structures are spores.

A coding sequence of this organism was used for carbon dioxide fixation:

glpX - Sedoheptulose 1,7-bisphosphatase

Domain	Bacteria
Phylum	Firmicutes
Class	Bacilli
Order	Bacillales
Family	Bacillaceae
Genus	Bacillus
Species	B. methanolicus

References

http://en.wikipedia.org/wiki/Escherichia_coli
http://nl.wikipedia.org/wiki/Melkzuurbacteri
http://en.wikipedia.org/wiki/Carboxysome
http://en.wikipedia.org/wiki/Bacillus_subtilis
http://en.wikipedia.org/wiki/Synechococcus
http://de.wikipedia.org/wiki/Actinobacillus
http://de.wikipedia.org/wiki/Corynebacterium_glutamicum
http://en.wikipedia.org/wiki/Bacillus

@@ Line 49: / Line 49: @@
 <div class="element" style="margin:10px; padding:10px; text-align:center; float:left; width:260px">
        <a href="https://static.igem.org/mediawiki/2014/2/25/Bielefeld-CeBiTec_2014-09-29_Ecoli.png" target="_blank"><img src="https://static.igem.org/mediawiki/2014/2/25/Bielefeld-CeBiTec_2014-09-29_Ecoli.png" width="250px"></a>
-       <br><font size="1" style="text-align:center;">Scanning electron micrograph of Escherichia coli, grown in culture and adhered to a cover slip.</font>
+       <br><font size="1" style="text-align:center;">Scanning electron micrograph of <i>Escherichia coli</i>, grown in culture and adhered to a cover slip.</font>
      </div>
 </td>
@@ Line 112: / Line 112: @@
 <div class="element" style="margin:10px 20px 10px 10px; padding:10px; text-align:center; float:right; width:260px">
        <a href="https://static.igem.org/mediawiki/2014/0/02/Bielefeld-CeBiTec_2014-09-29_Llactis.png" target="_blank"><img src="https://static.igem.org/mediawiki/2014/0/02/Bielefeld-CeBiTec_2014-09-29_Llactis.png" width="250px"></a>
-<br><font size="1" style="text-align:center;">Scanning Electron Micrograph of Enterococcus faecalis</font>
+<br><font size="1" style="text-align:center;">Scanning Electron Micrograph of <i>Enterococcus faecalis</i></font>
      </div>
 </td>
@@ Line 173: / Line 173: @@
 <div class="element" style="margin:10px 20px 10px 10px; padding:10px; text-align:center; float:left; width:260px">
        <a href="https://static.igem.org/mediawiki/2014/a/a5/Bielefeld-CeBiTec_2014-09-29_Hneapolitanus.png" target="_blank"><img src="https://static.igem.org/mediawiki/2014/a/a5/Bielefeld-CeBiTec_2014-09-29_Hneapolitanus.png" width="250px"></a>
-       <br><font size="1" style="text-align:center;">(A) Electron micrograph of Halothiobacillus neapolitanus cells, arrows highlight carboxysomes. (B) Image of intact carboxysomes isolated from H. neapolitanus. Scale bars indicate 100 nm.</font>
+       <br><font size="1" style="text-align:center;">(A) Electron micrograph of <i>Halothiobacillus neapolitanus</i> cells, arrows highlight carboxysomes. (B) Image of intact carboxysomes isolated from <i>H. neapolitanus</i>. Scale bars indicate 100 nm.</font>
      </div>
 </td>
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 <div class="element" style="margin:10px; padding:10px; text-align:center; float:left; width:260px">
        <a href="https://static.igem.org/mediawiki/2014/8/88/Bielefeld-CeBiTec_2014-09-29_Bsubtilis.png" target="_blank"><img src="https://static.igem.org/mediawiki/2014/8/88/Bielefeld-CeBiTec_2014-09-29_Bsubtilis.png" width="250px"></a>
-       <br><font size="1" style="text-align:center;">The Bacterium Bacillus subtilis taken with a Tecnai T-12 TEM. Taken by Allon Weiner, The Weizmann Institute of Science, Rehovot, Israel. 2006.</font>
+       <br><font size="1" style="text-align:center;">The Bacterium <i>Bacillus subtilis</i> taken with a Tecnai T-12 TEM. Taken by Allon Weiner, The Weizmann Institute of Science, Rehovot, Israel. 2006.</font>
      </div>
 </td>
@@ Line 296: / Line 296: @@
 <div class="element" style="margin:10px; padding:10px; text-align:center; float:left; width:260px">
        <a href="https://static.igem.org/mediawiki/2014/2/20/Bielefeld-CeBiTec_2014-09-29_Selongatus.png" target="_blank"><img src="https://static.igem.org/mediawiki/2014/2/20/Bielefeld-CeBiTec_2014-09-29_Selongatus.png" width="250px"></a>
-       <br><font size="1" style="text-align:center;">Synechococcus PCC 7002 in DIC microscopy. Imaging was performed with the Olympus BX61 microscope and a UPlanSApo 100× NA 1.40 oil immersion objective (Olympus). Pictures were acquired at room temperature in water with a camera (SPOT; Diagnostic Instruments, Inc.) using MetaMorph software (MDS Analytical Technologies).</font>
+       <br><font size="1" style="text-align:center;"><i>Synechococcus</i> PCC 7002 in DIC microscopy. Imaging was performed with the Olympus BX61 microscope and a UPlanSApo 100× NA 1.40 oil immersion objective (Olympus). Pictures were acquired at room temperature in water with a camera (SPOT; Diagnostic Instruments, Inc.) using MetaMorph software (MDS Analytical Technologies).</font>
      </div>
 </td>
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 <div class="element" style="margin:10px; padding:10px; text-align:center; float:left; width:260px">
        <a href="https://static.igem.org/mediawiki/2014/6/60/Bielefeld-CeBiTec_2014-09-30_Bmethanolicus.png" target="_blank"><img src="https://static.igem.org/mediawiki/2014/6/60/Bielefeld-CeBiTec_2014-09-30_Bmethanolicus.png" width="250px"></a>
-       <br><font size="1" style="text-align:center;">Microscopic image of Bacillus subtilis (ATCC 6633). Gram staining, magnification:1,000. The oval unstained structures are spores.</font>
+       <br><font size="1" style="text-align:center;">Microscopic image of <i>Bacillus subtilis</i> (ATCC 6633). Gram staining, magnification:1,000. The oval unstained structures are spores.</font>
      </div>
 </td>

Team:Bielefeld-CeBiTec/Notebook/Organisms

From 2014.igem.org

Revision as of 13:48, 5 October 2014

Organisms

References