Team:KIT-Kyoto/Test/Notebook/Labnote
From 2014.igem.org
(Difference between revisions)
Line 67: | Line 67: | ||
<td>pGEX6P-2</td> | <td>pGEX6P-2</td> | ||
<td>CuMTSE2</TD> | <td>CuMTSE2</TD> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BL21(DE3)</td> | ||
+ | <td>pGEX6P-2</td> | ||
+ | <td>CuMTS3</td> | ||
+ | <tr> | ||
+ | <td>BL21(DE3)</td> | ||
+ | <td>pGEX6P-2</td> | ||
+ | <td>CuMTS62</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <br> | ||
+ | <strong>Restriction Digest</strong> | ||
+ | <table class="materials"> | ||
+ | <tr> | ||
+ | <td rowspan="2">Host</td> | ||
+ | <td colspan="2">Sample</td> | ||
+ | <td rowspan="2">Enzyme</td> | ||
+ | <td rowspan="2">Buffer</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Vector</td> | ||
+ | <td>Insert</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BL21(DE3)PlysS</td> | ||
+ | <td>pGEX6P-2</td> | ||
+ | <td>CuMTSE1</td> | ||
+ | <td>EcoR1</td> | ||
+ | <td>Buffer H</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BL21(DE3)PlysS</td> | ||
+ | <td>pGEX6P-2</td> | ||
+ | <td>CuMTSE2</td> | ||
+ | <td>EcoR1</td> | ||
+ | <td>Buffer H</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BL21(DE3)PlysS</td> | ||
+ | <td>pGEX6P-2</td> | ||
+ | <td>CuMTS3</td> | ||
+ | <td>EcoR1</td> | ||
+ | <td>Buffer H</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BL21(DE3)PlysS</td> | ||
+ | <td>pGEX6P-2</td> | ||
+ | <td>CuMTSE62</td> | ||
+ | <td>EcoR1</td> | ||
+ | <td>Buffer H</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BL21(DE3)PlysS</td> | ||
+ | <td>pGEX6P-2</td> | ||
+ | <td>CuMTSE1</td> | ||
+ | <td>Sal1, Not1</td> | ||
+ | <td>Buffer H</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BL21(DE3)PlysS</td> | ||
+ | <td>pGEX6P-2</td> | ||
+ | <td>CuMTSE2</td> | ||
+ | <td>Sal1, Not1</td> | ||
+ | <td>Buffer H</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BL21(DE3)PlysS</td> | ||
+ | <td>pGEX6P-2</td> | ||
+ | <td>CuMTS3</td> | ||
+ | <td>Sal1, Not1</td> | ||
+ | <td>Buffer H</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BL21(DE3)PlysS</td> | ||
+ | <td>pGEX6P-2</td> | ||
+ | <td>CuMTSE62</td> | ||
+ | <td>Sal1, Not1</td> | ||
+ | <td>Buffer H</td> | ||
</tr> | </tr> | ||
</table> | </table> |
Revision as of 06:08, 2 October 2014
LabNote
June
June 10, 2014
OKAMOTO Miniprep
Host | Sample | |
Vector | Insert | |
BL21(DE3) | pGEX6P-2 | CuMTSE1 |
BL21(DE3) | pGEX6P-2 | CuMTSE2 |
BL21(DE3) | pGEX6P-2 | CuMTS3 |
BL21(DE3) | pGEX6P-2 | CuMTS62 |
Restriction Digest
Host | Sample | Enzyme | Buffer | |
Vector | Insert | |||
BL21(DE3)PlysS | pGEX6P-2 | CuMTSE1 | EcoR1 | Buffer H |
BL21(DE3)PlysS | pGEX6P-2 | CuMTSE2 | EcoR1 | Buffer H |
BL21(DE3)PlysS | pGEX6P-2 | CuMTS3 | EcoR1 | Buffer H |
BL21(DE3)PlysS | pGEX6P-2 | CuMTSE62 | EcoR1 | Buffer H |
BL21(DE3)PlysS | pGEX6P-2 | CuMTSE1 | Sal1, Not1 | Buffer H |
BL21(DE3)PlysS | pGEX6P-2 | CuMTSE2 | Sal1, Not1 | Buffer H |
BL21(DE3)PlysS | pGEX6P-2 | CuMTS3 | Sal1, Not1 | Buffer H |
BL21(DE3)PlysS | pGEX6P-2 | CuMTSE62 | Sal1, Not1 | Buffer H |
July
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