Team:NRP-UEA-Norwich/Project System
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<li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/Project_System">System</a></li> | <li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/Project_System">System</a></li> | ||
<li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/Project_Parts">Parts</a></li> | <li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/Project_Parts">Parts</a></li> | ||
- | <li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/Project_GG-Cloning"> | + | <li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/Project_GG-Cloning">Golden Gate cloning</a></li> |
<li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/Project_Mo-Flipper">Golden Gate Modular Flipper</a></li> | <li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/Project_Mo-Flipper">Golden Gate Modular Flipper</a></li> | ||
+ | <li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/HP_Collaborations">Collaborations</a></li> | ||
</ul> | </ul> | ||
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<li class="dropdown"> | <li class="dropdown"> | ||
- | <a data-toggle="dropdown" class="dropdown-toggle" href="#"> | + | <a data-toggle="dropdown" class="dropdown-toggle" href="#">Policy and Practices<span class="caret"></span></a> |
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
<li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/HP">Overview</a></li> | <li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/HP">Overview</a></li> | ||
<li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/HP_CUT">The CUT event</a></li> | <li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/HP_CUT">The CUT event</a></li> | ||
- | <li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/HP_School-Events">School | + | <li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/HP_School-Events">The Hewett School</a></li> |
- | + | ||
- | + | ||
<li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/HP_Science-Cafe">Science Cafe</a></li> | <li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/HP_Science-Cafe">Science Cafe</a></li> | ||
- | <li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/HP_Ethics">Ethics | + | <li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/HP_Ethics">Ethics of Public Consultation</a></li> |
- | + | ||
</ul> | </ul> | ||
</li> | </li> |
Revision as of 11:04, 9 October 2014
Our System
Chassis'
Escherichia coli
We have used E. coli as a chassis organism to allow us to clone our gene construct successfully. After using Golden Gate cloning to make our Level 1 and Level 2 constructs, we needed to transform E. coli with the newly made constructs. This is to ensure that we have enough of the correct construct when we get to the next stage of transforming Agrobacterium tumefaciens.
Agrobacterium tumefaciens
A. Tumefaciens (Agro) was a chassis organism in this project to act as a shuttle chassis for the delivery of our Level 1 and Level 2 DNA constructs into Nicotiana benthamiana. Agro infects the plant through its Ti plasmid. This inserts a segment of its DNA, T-DNA, into the host’s genome. This allows the expression of the gene of interest.