Team:Hong Kong HKUST/wetlab

From 2014.igem.org

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<h2>Wetlab</h2>
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<h2>Protocols</h2>
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<p>The wet lab started at the beginning of summer and lasted for three months. Our wet lab work is divided into four parts, namely:
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<p>We have a variety of protocols that we followed for working on our project. The step-by-step procedures of each technique
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(1) (Detecting Module) ComD & ComE, (2) (Detecting Module) P<sub><i>comCDE</i></sub>, (3) (Regulation Module) ComX & ComW, and
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  can be found here!
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(4) (Regulation Module) combox. During the summer, lab work such as primer design, digestion, and ligation was carried out extensively
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and useful data were collected. These can be retrieved from Notebook and Protocols pages for details of experimental flows.
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The BioBrick, researcher, environmental and university safety issues can be found in Safety page. In terms of project process,
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some experiments could not be completed due to time constrains and they are outlined in Future Work page.
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<h4> Protocol</h4>
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<h4> Mutagenesis</h4>
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<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
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<p> Protocol description/picture here. </p>
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visibly touching the cheekbone below the eye. </p>
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<h4> Western Blot </h4>
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<p> Protocol description/picture here. </p>
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<h4> LB agar and LB plates </h4>
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<p> Protocol description/picture here. </p>
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<h4> Notebook </h4>
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<h4> Ligation </h4>
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<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
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<p> Protocol description/picture here. </p>
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visibly touching the cheekbone below the eye. </p>
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<h4> Safety </h4>
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<h4> Ethanol Precipitation</h4>
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<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
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<p> Protocol description/picture here. </p>
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visibly touching the cheekbone below the eye. </p>
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<h4> Pbad protocols </h4>
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<p>Protocol description/picture here. </p>
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<h4> Gibson Assembly </h4>
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<p> Protocol description/picture here. </p>
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<h4> Polymerase Chain Reaction (PCR)</h4>
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<p> Protocol description/picture here. </p>
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Revision as of 15:02, 30 September 2014

Protocols

We have a variety of protocols that we followed for working on our project. The step-by-step procedures of each technique can be found here!

Home

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Main Project

Side Project

Human Practice

Wetlab

Achievement

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