Team:Bielefeld-CeBiTec/Notebook/Organisms

From 2014.igem.org

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<li>frd - Fumarate reductase</li>
<li>frd - Fumarate reductase</li>
<li>Cytochromes</li>
<li>Cytochromes</li>
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<li>adhA - Alcohol dehydrogenase</li>
 
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Revision as of 10:00, 4 October 2014


Organisms

Escherichia coli
Escherichia coli


The strain KRX is used for all approaches. Coding sequences of this organism were used for different purposes:

  • frd - Fumarate reductase
  • Cytochromes
DomainBacteria
PhylumProteobacteria
ClassGammaproteobacteria
OrderEnterobacteriales
FamilyEnterobacteriaceae
GenusEscherichia
SpeciesE. coli
Lactococcus lactis
Lactococcus lactis


Coding sequences of this organism were used for the isobutanol production:

  • kivD - α-ketoisovalerate decarboxylase
  • adhA - Alcoholdehydrogenase
DomainBacteria
PhylumFirmicutes
ClassBacilli
OrderLactobacillales
FamilyStreptococcaceae
GenusLactococcus
SpeciesL. lactis
Halothiobacillus neapolitanus
Halothiobacillus neapolitanus


Coding sequences of this organism were used for the carboxysome:

  • sap - shell associated protein
  • can - carbonic anhydrase
  • csoS1-4 - shell protein
  • csoS1D - pore protein
DomainBacteria
PhylumProteobacteria
Classγ-Proteobacteria
OrderChromatiales
FamilyHalothiobacillaceae
GenusHalothiobacillus
SpeciesH. neapolitanus
Bacillus subtilis
Bacillus subtilis


A coding sequence of this organism was used for the isobutanol production:

  • alsS - α-acetolactate synthase
DomainBacteria
PhylumFirmicutes
ClassBacilli
OrderBacillales
FamilyBacillaceae
GenusBacillus
SpeciesB. subtilis
Synechococcus elongatus
Synechococcus elongatus


A coding sequence of this organism was used for the carbon dioxide fixation:

  • RuBisCO - Ribulose 1,5-bisphosphate carboxylase / oxygenase
  • prkA - Phosphoribulokinase
DomainBacteria
PhylumCyanobacteria
ClassOscillatoriophycideae
OrderChroococcales
GenusSynechococcus
SpeciesS. elongatus
Actinobacillus succinogenes
Actinobacillus succinogenes


A coding sequence of this organism was used for the transport of reduction equivalents:

  • Asuc_1813/1814/1815/1816 - fumarate reductase
DomainBacteria
PhylumProteobacteria
Classγ-Proteobacteria
OrderPasteurellales
FamilyPasteurellaceae
GenusActinobacillus
SpeciesA. succinogenes
Geobacter sulfurreducens
Geobacter sulfurreducens


A coding sequence of this organism was used for the transport of electrons:

  • Cytochromes
DomainBacteria
PhylumProteobacteria
Subphylumδ / ε-Proteobacteria
Classδ-Proteobacteria
OrderDesulfuromonadales
FamilyGeobacteraceae
GenusGeobacter
SpeciesG. sulfurreducens
Corynebacterium glutamicum
Corynebacterium glutamicum


A coding sequence of this organism was used for an enzyme assay:

  • pgk - Phosphoglycerate kinase
DomainBacteria
PhylumActinobacteria
ClassActinobacteridae
OrderActinomycetales
SuborderCorynebacterineae
FamilyCorynebacteriaceae
GenusCorynebacterium
SpeciesC. glutamicum
Bacillus methanolicus
Bacillus methanolicus


A coding sequence of this organism was used for carbon dioxide fixation:

  • glpX - Sedoheptulose 1,7-bisphosphatase
DomainBacteria
PhylumFirmicutes
ClassBacilli
OrderBacillales
FamilyBacillaceae
GenusBacillus
SpeciesB. methanolicus

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