Team:Bielefeld-CeBiTec/Notebook/Organisms

From 2014.igem.org

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        <div class="tab" id="B.methanolicus">
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                <a href="#B.methanolicus"><i>Bacillus methanolicus</i></a>
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<a href="https://static.igem.org/mediawiki/2014/ .pdf" target="_blank"> <img src="https://static.igem.org/mediawiki/2014/b/bd/Bielefeld-CeBiTec_2014-08-12_pdf_Icon.png" height="30px"> </a>
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                <a  style="font-size:24px" href="#"><h6><i>Bacillus methanolicus</i>  <a href="https://static.igem.org/mediawiki/2014/ .pdf" target="_blank"> <img src="https://static.igem.org/mediawiki/2014/b/bd/Bielefeld-CeBiTec_2014-08-12_pdf_Icon.png" height="30px"> </a></h6></a>
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<p><br>A coding sequence of this organism was used for carbon dioxide fixation:
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<li>glpX - Sedoheptulose 1,7-bisphosphatase</li>
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                      <td><b>Domain</b></td><td>Bacteria</td>
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                      <td><b>Phylum</b></td><td>Firmicutes</td>
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                    </tr>
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                    <tr>
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                      <td><b>Class</b></td><td>Bacilli</td>
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                    </tr>
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                    <tr>
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                      <td><b>Order</b></td><td>Bacillales</td>
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                    </tr>
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                    <tr>
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                      <td><b>Family</b></td><td>Bacillaceae</td>
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                    </tr>
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                    <tr>
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                      <td><b>Genus</b></td><td>Bacillus</td>
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                    </tr>
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                    <tr>
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                      <td><b>Species</b></td><td>B. methanolicus</td>
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Revision as of 11:02, 30 September 2014


Organisms


The strain KRX is used for all approaches. Coding sequences of this organism were used for different purposes:

  • frd - Fumarate reductase
  • Cytochromes
  • adhA - Alcohol dehydrogenase

DomainBacteria
PhylumProteobacteria
ClassGammaproteobacteria
OrderEnterobacteriales
FamilyEnterobacteriaceae
GenusEscherichia
SpeciesE. coli


Coding sequences of this organism were used for the isobutanol production:

  • kivD - α-ketoisovalerate decarboxylase
  • adhA - Alcoholdehydrogenase

DomainBacteria
PhylumFirmicutes
ClassBacilli
OrderLactobacillales
FamilyStreptococcaceae
GenusLactococcus
SpeciesL. lactis


Coding sequences of this organism were used for the carboxysome:

  • sap - shell associated protein
  • can - carbonic anhydrase
  • csoS1-4 - shell protein
  • csoS1D - pore protein

DomainBacteria
PhylumProteobacteria
Classγ-Proteobacteria
OrderChromatiales
FamilyHalothiobacillaceae
GenusHalothiobacillus
SpeciesH. neapolitanus


A coding sequence of this organism was used for the isobutanol production:

  • alsS - α-acetolactate synthase

DomainBacteria
PhylumFirmicutes
ClassBacilli
OrderBacillales
FamilyBacillaceae
GenusBacillus
SpeciesB. subtilis


A coding sequence of this organism was used for the carbon dioxide fixation:

  • RuBisCO - Ribulose 1,5-bisphosphate carboxylase / oxygenase
  • prkA - Phosphoribulokinase

DomainBacteria
PhylumCyanobacteria
ClassOscillatoriophycideae
OrderChroococcales
GenusSynechococcus
SpeciesS. elongatus


A coding sequence of this organism was used for the transport of reduction equivalents:

  • Asuc_1813/1814/1815/1816 - fumarate reductase

DomainBacteria
PhylumProteobacteria
Classγ-Proteobacteria
OrderPasteurellales
FamilyPasteurellaceae
GenusActinobacillus
SpeciesA. succinogenes
DomainBacteria
PhylumProteobacteria
Subphylumδ / ε-Proteobacteria
Classδ-Proteobacteria
OrderDesulfuromonadales
FamilyGeobacteraceae
GenusGeobacter
SpeciesG. sulfurreducens












A coding sequence of this organism was used for an enzyme assay:

  • pgk - Phosphoglycerate kinase

DomainBacteria
PhylumActinobacteria
ClassActinobacteridae
OrderActinomycetales
SuborderCorynebacterineae
FamilyCorynebacteriaceae
GenusCorynebacterium
SpeciesC. glutamicum


A coding sequence of this organism was used for carbon dioxide fixation:

  • glpX - Sedoheptulose 1,7-bisphosphatase

DomainBacteria
PhylumFirmicutes
ClassBacilli
OrderBacillales
FamilyBacillaceae
GenusBacillus
SpeciesB. methanolicus